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DNCB로 유발된 생쥐의 아토피 피부염에 대한 지실 (枳實)의 효능

Effects of Aurantii Immaturus Fructus (AI) on Atopic Dermatitis (AD) Induced by DNCB in Mice

박젬마|채중원
대한한방소아과학회 (The Association of Pediatrics of Korean Medicine)|2015.02|대한한방소아과학회지 (The Journal of Pediatrics of Korean Medicine)|제29권 제1호|pp. 27-43

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참고문헌

키워드

2Atopic dermatitis (AD)4-dinitrochlorobenzene (DNCB)Aurantii Immaturus Fructus (AI)
주제분류한의학

초록

ObjectivesThe purpose of this study was to investigate the effects of AI on AD induced by DNCB in mice. AI hasantiallergic property that is useful in treating allergy-related-diseases, such as asthma, anaphylactic shock, acutebronchitis and skin diseases, skin pruritus from gastrointestinal diseases. However, AI has not been studiedintensively yet regarding anti-inflammatory effect on AD. Therefore, this study was conducted on 2,4-dinitrochlorobezene(DNCB)-induced mice to investigate effects of AI in AD. MethodsIn the experiment, we divided mice into four groups: a normal group (NOR), a control group (CON), an AIspread group (AI spread), and an AI spread and feeding group (AI spread & feeding). Then examined the changesin the body weight, weights of spleen and ear, thickness of dorsum skin and ear skin, clinical aspects on dorsumskin, historical assessments, proliferation of splenocytes in vitro and in vivo, and cytokine (TNF-α, IL-10). ResultsFrom the experiment, the ear weight of AI spread & feeding group was significantly dropped and the earthickness of both AI spread and AI spread & feeding were decreased significantly. Dorsum skin thickness was alsodecreased significantly in both AI spread and AI spread & feeding group. Also, AI treatment improved thesymptoms of AD, such as coloration, erythema and desquamation and had a better effect on AI spread & feedinggroup. In histopathological observation, thickened epidermis, hyperkeratosis, pigmentation, hypergranulosis,parakeratosis were diminished as well in both AI spread and AI spread & feeding group. In vitro, we could observewhen AI was increased as proliferation rate of splenocytes were increased, too. ConclusionsIn conclusion, these data suggest that AI can decrease symptoms of AD and show AI can be useful herbal therapyfor AD.

Abstract

ObjectivesThe purpose of this study was to investigate the effects of AI on AD induced by DNCB in mice. AI hasantiallergic property that is useful in treating allergy-related-diseases, such as asthma, anaphylactic shock, acutebronchitis and skin diseases, skin pruritus from gastrointestinal diseases. However, AI has not been studiedintensively yet regarding anti-inflammatory effect on AD. Therefore, this study was conducted on 2,4-dinitrochlorobezene(DNCB)-induced mice to investigate effects of AI in AD. MethodsIn the experiment, we divided mice into four groups: a normal group (NOR), a control group (CON), an AIspread group (AI spread), and an AI spread and feeding group (AI spread & feeding). Then examined the changesin the body weight, weights of spleen and ear, thickness of dorsum skin and ear skin, clinical aspects on dorsumskin, historical assessments, proliferation of splenocytes in vitro and in vivo, and cytokine (TNF-α, IL-10). ResultsFrom the experiment, the ear weight of AI spread & feeding group was significantly dropped and the earthickness of both AI spread and AI spread & feeding were decreased significantly. Dorsum skin thickness was alsodecreased significantly in both AI spread and AI spread & feeding group. Also, AI treatment improved thesymptoms of AD, such as coloration, erythema and desquamation and had a better effect on AI spread & feedinggroup. In histopathological observation, thickened epidermis, hyperkeratosis, pigmentation, hypergranulosis,parakeratosis were diminished as well in both AI spread and AI spread & feeding group. In vitro, we could observewhen AI was increased as proliferation rate of splenocytes were increased, too. ConclusionsIn conclusion, these data suggest that AI can decrease symptoms of AD and show AI can be useful herbal therapyfor AD.

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