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마황 추출물 투여가 Ovalbumin으로 유발된 마우스 알레르기성 천식에 미치는 영향

Effects of Ephedra sinica (ES) Extract on the Ovalbumin-Induced Allergid Asthma in Mice

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Objective : Ephedra sinica (ES) has has been used as remedy of allergic diseases for a long time in Korea. Inthe present study, we investigated the anti-allergic effects of ES on experimental allergic asthma mouse modelusing ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 ㎎ of aluminum potassiumsulfate of 0.2 ㎖ phosphate-buffered saline(PBS) intraperitoneally on day 1 and 15. mice were challenged on 3consecutive days with 5% OVA and AHR was assessed 24 h after the last challenge. we examined the lunghistology, airway hyper sensitivity, total inflammatory cell count in bronchoaveloar lavage fluid(BALF),Th2-associated cytokines production and IgE production. Results : ES potently inhibited the lung damage and the development of Penh. ES also reduced the number ofBAL cells during OVA-induced allergic asthma. Furthermore, ES inhibited cytokines production such as IL-4, IL-13productions, and IgE level of serum. Conclusion : These results suggest that ES may inhibit the production of IL-4, IL-13, IgE and infiltration ofinflammatory cell and be beneficial oriental medicine for allergic asthma.

Abstract

Objective : Ephedra sinica (ES) has has been used as remedy of allergic diseases for a long time in Korea. Inthe present study, we investigated the anti-allergic effects of ES on experimental allergic asthma mouse modelusing ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 ㎎ of aluminum potassiumsulfate of 0.2 ㎖ phosphate-buffered saline(PBS) intraperitoneally on day 1 and 15. mice were challenged on 3consecutive days with 5% OVA and AHR was assessed 24 h after the last challenge. we examined the lunghistology, airway hyper sensitivity, total inflammatory cell count in bronchoaveloar lavage fluid(BALF),Th2-associated cytokines production and IgE production. Results : ES potently inhibited the lung damage and the development of Penh. ES also reduced the number ofBAL cells during OVA-induced allergic asthma. Furthermore, ES inhibited cytokines production such as IL-4, IL-13productions, and IgE level of serum. Conclusion : These results suggest that ES may inhibit the production of IL-4, IL-13, IgE and infiltration ofinflammatory cell and be beneficial oriental medicine for allergic asthma.

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