감초 추출물 투여가 Ovalbumin으로 유발된마우스 알레르기성 천식에 미치는 영향
Effects of Glycyrrhiza uralensis Fisch (GUF) Extract on the Ovalbumin-Induced Allergid Asthma in Mice
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Objective : Glycyrrhiza uralensis Fisch (GUF) has been used as remedy of allergic diseases for a long time inKorea. In the present study, we investigated the anti-allergic effects of GUF on experimental allergic asthma mousemodel using ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 ㎎ of aluminum potassiumsulfate of 0.2 ㎖ phosphate-buffered saline (PBS) intraperitoneally on day 1 and 15. Mice were challenged on 3consecutive days with 5% OVA and AHR was assessed 24 hrs after the last challenge. We examined totalinflammatory cell number in bronchoaveloar lavage fluid (BALF), Th2-associated cytokine productions and lunghistology. Results : GUF potently inhibited the development of airway hypersensitivity and also reduced the number ofBAL cells during OVA-induced allergic asthma. GUF also inhibited cytokine productions such as IL-4, IL-13 in lungtissue. Furthermore, GUF treatment inhibited allergic airway inflammation. Conclusion : These results suggest that GUF may inhibit the production of IL-4, IL-13 and infiltration ofinflammatory cell and be beneficial oriental medicine for allergic asthma.
Abstract
Objective : Glycyrrhiza uralensis Fisch (GUF) has been used as remedy of allergic diseases for a long time inKorea. In the present study, we investigated the anti-allergic effects of GUF on experimental allergic asthma mousemodel using ovalbumin (OVA). Methods : BALB/c mice were sensitized and challenged with 100 ug of OVA and 1 ㎎ of aluminum potassiumsulfate of 0.2 ㎖ phosphate-buffered saline (PBS) intraperitoneally on day 1 and 15. Mice were challenged on 3consecutive days with 5% OVA and AHR was assessed 24 hrs after the last challenge. We examined totalinflammatory cell number in bronchoaveloar lavage fluid (BALF), Th2-associated cytokine productions and lunghistology. Results : GUF potently inhibited the development of airway hypersensitivity and also reduced the number ofBAL cells during OVA-induced allergic asthma. GUF also inhibited cytokine productions such as IL-4, IL-13 in lungtissue. Furthermore, GUF treatment inhibited allergic airway inflammation. Conclusion : These results suggest that GUF may inhibit the production of IL-4, IL-13 and infiltration ofinflammatory cell and be beneficial oriental medicine for allergic asthma.
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