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湯火止痛散의 NF-κB 및 MAPK 억제를 통한 항염증 효과

Anti-inflammatory Effects of Tanghwajitong-san through Inhibition of NF-κB and MAPK

지선영|황보민|고민정
대한한방안이비인후피부과학회 (The Korean Medicine Opthalmology&Otolaryngology&Dermatology Society)|2023.02|한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology&Otorhinolaryngology&Dermatology)|제36권 제1호|pp. 1-20

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NF-κBMAPKInflammationTanghwajitong-sanAngelicae Gigntis RadixRhei Rhizoma
주제분류한의학

초록

Objectives : The purpose of this study was to evaluate the anti-inflammatory effect of Tanghwajitongsan(THJTS) through inhibition of NF-κB and MAPK. Methods : We evaluated cell survival rate by MTT assay, NO production by nitrite content in the culture mediu m. We quantified TNF-α, IL-1β, IL-6 and PGE 2 of the cultured supernatant by ELISA. And we evaluated the effect of THJTS on protein expression of NF-κB, MAPK, iNOS and COX-2 by Western blot analysis. THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-infl ammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activated alterations in protein expres sion of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein expression of COX-2. Results :　THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-inflammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activat ed alterations in protein expression of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein ex pression of COX-2. Conclusions : This study can provide scientific evidence for the anti-inflammatory effects and underlying mech anisms of THJTS.

Abstract

Objectives : The purpose of this study was to evaluate the anti-inflammatory effect of Tanghwajitongsan(THJTS) through inhibition of NF-κB and MAPK. Methods : We evaluated cell survival rate by MTT assay, NO production by nitrite content in the culture mediu m. We quantified TNF-α, IL-1β, IL-6 and PGE 2 of the cultured supernatant by ELISA. And we evaluated the effect of THJTS on protein expression of NF-κB, MAPK, iNOS and COX-2 by Western blot analysis. THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-infl ammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activated alterations in protein expres sion of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein expression of COX-2. Results :　THJTS ameliorates LPS-activated alterations in protein expression of NF-κB, p-38, iNOS and COX-2 and production of NO, pro-inflammatory cytokines and PGE 2 . Also, THJTS ameliorates LOX, PGN and FLA-activat ed alterations in protein expression of NO, iNOS. THJTS ameliorates only PGN-activated alterations in protein ex pression of COX-2. Conclusions : This study can provide scientific evidence for the anti-inflammatory effects and underlying mech anisms of THJTS.

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