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신경근전기자극이 체중 부하를 제거한 신생 흰쥐 골격근 조직의 MEF2C 및 VEGF 발현에 미치는 영향

Influence of Neuromuscular Electrical Stimulation on MEF2C and VEGF Expression of Neonatal Rat Skeletal Muscle During Suspension Unloading

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초록

The aim of this study was to identify the effect of suspension unloading (SU) and electrical stimulation upon the development of neonatal muscular system. For this study, the neonatal rats were randomly divided into three groups: a control group, an experimental group Ⅰ, and an experimental group Ⅱ. The SU for experimental group Ⅰ and Ⅱ was applied from postnatal day (PD) 5 to PD 30. The electrical stimulation for soleus muscle of experimental group Ⅱwas applied from PD 16 to PD 30 using neuromuscular electrical stimulation (NMES), which gave isometric contraction with 10 pps for 30 minutes twice a day. In order to observe the effect of SU and ES, this study observed myocyte enhancer factor 2C (MEF2C) and vascular endothelial growth factor (VEGF) immunoreactivity in the soleus muscles at PD 15 and PD 30. In addition, the motor behavior test was performed through footprint analysis at PD 30. The following is the result. At PD 15, the soleus muscles of experimental group Ⅰand Ⅱ had significantly lower MEF2C, VEGF immunoreactivity than the control group. It proved that microgravity conditions restricted the development of the skeletal muscle cells at PD 15. At PD 30, soleus muscles of the control group and experimental group Ⅱ had significantly higher MEF2C, VEGF, immunoreactivity than experimental group Ⅰ. It proved that the NMES facilitated the development of the skeletal muscle cells. At PD 30, it showed that SU caused the decrease in stride length of parameter of gait analysis and an increase in toe-out angle, and that the NMES decreased these variations. These results suggest that weight bearing during neonatal developmental period is essential for muscular development. They also reveal that NMES can encourage the development of muscular systems by fully supplementing the effect of weight bearing, which is an essential factor in the neonatal developmental process.

Abstract

The aim of this study was to identify the effect of suspension unloading (SU) and electrical stimulation upon the development of neonatal muscular system. For this study, the neonatal rats were randomly divided into three groups: a control group, an experimental group Ⅰ, and an experimental group Ⅱ. The SU for experimental group Ⅰ and Ⅱ was applied from postnatal day (PD) 5 to PD 30. The electrical stimulation for soleus muscle of experimental group Ⅱwas applied from PD 16 to PD 30 using neuromuscular electrical stimulation (NMES), which gave isometric contraction with 10 pps for 30 minutes twice a day. In order to observe the effect of SU and ES, this study observed myocyte enhancer factor 2C (MEF2C) and vascular endothelial growth factor (VEGF) immunoreactivity in the soleus muscles at PD 15 and PD 30. In addition, the motor behavior test was performed through footprint analysis at PD 30. The following is the result. At PD 15, the soleus muscles of experimental group Ⅰand Ⅱ had significantly lower MEF2C, VEGF immunoreactivity than the control group. It proved that microgravity conditions restricted the development of the skeletal muscle cells at PD 15. At PD 30, soleus muscles of the control group and experimental group Ⅱ had significantly higher MEF2C, VEGF, immunoreactivity than experimental group Ⅰ. It proved that the NMES facilitated the development of the skeletal muscle cells. At PD 30, it showed that SU caused the decrease in stride length of parameter of gait analysis and an increase in toe-out angle, and that the NMES decreased these variations. These results suggest that weight bearing during neonatal developmental period is essential for muscular development. They also reveal that NMES can encourage the development of muscular systems by fully supplementing the effect of weight bearing, which is an essential factor in the neonatal developmental process.

참고문헌 (42)

  1. Bacic M, "Differential expression of vascular endothelial growth factor (vascular permeability factor) forms in rat tissues", 12(1), pp.11~15 (1995)
  2. Bastide B, "Properties of ryanodine receptor in rat muscles submitted to unloaded conditions", 270(2), pp.442~447 (2000)
  3. Belanger M, "Electrical stimulation: Can it increase muscle strength and reverse osteopenia in spinal cord injured individuals", 81(8), pp.1090~1098 (2000)
  4. Bi W, "The transcription factor MEF2C-null mouse exhibits complex vascular malformations and reduced cardiac expression of angiopoietin 1 and VEGF", 211, pp.255~267 (1999)
  5. Breen EC, "Angiogenic growth factor mRNA responses in muscle to a single bout of exercise", 81(1), pp.355~361 (1996)
  6. Breier G, "The role of vascular endothelial growth factor in blood vessel formation", 6(12), pp.454~456 (1996)
  7. Caiozzo VJ, "Microgravity-induced transformations of myosin isoforms and contractile properties of skeletal muscle", 81(1), pp.123~132 (1996)
  8. Caldwell JH, "The effects of deafferentation and spinal cord transection on synapse elimination in developing rat muscles", pp.145~159 (1983)
  9. De Angelis L, "Skeletal myogenic progenitors originating from embryonic dorsal aorta coexpress endothelial and myogenic markers and contribute to postnatal muscle growth and regeneration", 147(4), pp.869~878 (1999)
  10. Dedkov EI, "Resistance vessel remodeling and reparative angiogenesis in the microcirculatory bed of long-term denervated skeletal muscles", 63, pp.96~114 (2002)
  11. Glaser JA, "Electrical muscle stimulation as an adjunct to exercise therapy in the treatment of nonacute low back pain: A randomized trial", 2(5), pp.295~299 (2001)
  12. Greensmith DL, "Mechanical activity is necessary for the elimination of polyneuronal innervation of developing rat soleus muscles", 110(1), pp.131~134 (1998)
  13. Gustafsson T, "Exercise-induced expression of angiogenesis-related transcription and growth factors in human skeletal muscle", 276, pp.H679~H685 (1999)
  14. Hang J, "VEGF gene expression is upregulated in electrically stimulated rat skeletal muscle", 269, pp.H1827~H1831 (1995)
  15. Herbison GJ, "Muscle atrophy in rats following denervation", ditun(jf), pp.401~404 (1979)
  16. Huckstorf BL, "Effects of hindlimb unloading on neuromuscular development of neonatal rats", 119, pp.169~178 (2000)
  17. Inobe M, "Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscle", 92(5), pp.1936~1942 (2002)
  18. Ishii H, Oota I, "Differential gene expression of vascular endothelial growth factor isoforms and their receptors in the development of the rat masseter muscle", 27(7), pp.505~510 (2002)
  19. Kablar B, "Information provided by the skeletal muscle and associated neurons is necessary for proper brain development", 20(7), pp.573~584 (2002)
  20. Kaplan RE, "Electrical foot stimulation and implications for the prevention of venous thromboembolic disease", 88(2), pp.200~204 (2002)
  21. Keil L, "Pituitary oxytocin and vasopressin content of rats flown on COSMOS 2044", pp.2~ (1992)
  22. Kerai B, "Effect of transient neonatal muscle paralysis on the growth of soleus motoneurons in the rat", 85(1), pp.89~95 (1995)
  23. Lee RC, "A review of the biophysical basis for the clinical application of electric fields in soft-tissue repair", pp.319~335 (1993)
  24. Leivo I, "Abnormal mitochondria and sarcoplasmic changes in rabbit skeletal muscle induced by immobilization", 106(12), pp.1113~1123 (1998)
  25. Mercier C, "Effects of hindlimb suspension on contractile properties of young and old rat muscles and the impact of electrical stimulation on the recovery process", 106(3), pp.305~320 (1999)
  26. Mercier C, "Influence of electrical stimulation on enzymatic activity of old rat muscles during hindlimb suspension", pp.1 117~3 124 (1993)
  27. Metz GA, "The effects of acute and chronic stress on motor and sensory performance in male Lewis rats", 72(1-2), pp.29~35 (2001)
  28. Mozdziak PE, "Unloading of juvenile muscle results in a reduced muscle size 9 wk after reloading", 88(1), pp.158~164 (2000)
  29. Ogunshola OO, "Neuronal VEGF expression correlates with angiogenesis in postnatal developing rat brain", 119(1), pp.139~153 (2000)
  30. Pette D, "The adaptive potential of skeletal muscle fibers", 27(4), pp.423~448 (2002)
  31. Qin L, "Electrical stimulation prevents immobilization atrophy in skeletal muscle of rabbits", 78(5), pp.512~517 (1997)
  32. Rehfeldt C, "Myogenesis and postnatal skeletal muscle cell growth as influenced by selection", 66, pp.177~188 (2000)
  33. Schiaffino S, "Calcineurin signaling and neural control of skeletal muscle fiber type and size", 23(12), pp.569~575 (2002)
  34. Schmalbruch H, "Skeletal Muscle"
  35. Sinha RK, "The effect of a 5-day space flight on the immature rat spine", 2(4), pp.239~243 (2002)
  36. Taillandier D, "Regulation of proteolysis during reloading of the unweighted soleus muscle", 35(5), pp.665~675 (2003)
  37. Vanderthommen M, "Blood flow variation in human muscle during electrically stimulated exercise bouts", 83(7), pp.936~941 (2002)
  38. Varejao AS, "Toe out angle: A functional index for the evaluation of sciatic nerve recovery in the rat model", 183, pp.695~699 (2003)
  39. Walton K, "Postnatal development under conditions of stimulated weightlessness and space flight", 28(1-2), pp.25~34 (1998)
  40. Wang JS, "Neuromuscular electrical stimulation enhances endothelial vascular control and hemodynamic function in paretic upper extremities of patients with stroke", 85(7), pp.1112~1116 (2004)
  41. Yamakuchi M, "Type Ⅰ muscle atrophy caused by microgravity-induced decrease of myocyte enhancer factor 2C (MEF2C) protein expression", 477(1-2), pp.135~140 (2000)
  42. Zerath E, "Bone effects of 13 days of weightlessness on rat and monkey some results of biocosmos 1887 and ground simulations", (gues c), pp.1~ (1990)

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