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좌골신경손상 쥐 모델을 이용한 미세전류 자극의 근위축 억제 효과 확인 및 자극 세기 별 비교

The Effect of Microcurrent Electrical Stimulation on Muscle Atrophy Suppression in a Sciatic Nerve Injured Rat Model; Comparative Study by Current Intensity

김서현|김택중|김세빈|최수임|곽호영|김한성|이한아|장승준|황동현
대한의용생체공학회 (The Korea Society Of Medical & Biological Engineering)|2017.08|의공학회지 (Journal of biomedical Engineering Research)|제38권 제4호|pp. 175-182

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키워드

Micro-CTSciatic nerve injuryMuscle atrophyMicro-current therapy

초록

Microcurrent electrical stimulation(MES) has been used to accelerate recovery of atrophied skeletal muscle. However, convincing stimulation parameters for suppressing muscle atrophy due to injured sciatic nerve remains unclear. The objective of this study was to investigate the effective intensity of MES on restraining muscle atrophy with rat model underwent sciatic nerve injury(SNI). Twenty-5-week-old Sprague Dawley male rats were equally assigned to five groups : Control group(Control, CON, n = 4), Denervation group(Denervation, D, n = 4), Denervation with MES of 22 μA group(Denervation + 22 μA, D+22, n = 4), Denervation with MES of 100 μA group (Denervation + 100 μA, D+100 n = 4), Denervation with MES of 400 μA group(Denervation + 400 μA, D+400, n = 4). To induce muscle atrophy, all rats in the D, D+22, D+100, and D+400 groups, were subjected to sciatic nerve injury on their right hindlimb and allowed to have 1 week of resting period. Following this period, rats underwent daily MES(60 min/ a day, 5times/1week) for 4 weeks. After that, we investigate morphological changes in muscle volume by using in vivo micro-computed tomography at week 0, 1, 3 and 5. After 5 weeks, the muscle volume had the highest value in D+400 group, and also noticeably increased in D+100 group compared to it in D group. The results of this study imply that MES with current intensities between 100-400 μA can suppress muscle atrophy effectively.

Abstract

Microcurrent electrical stimulation(MES) has been used to accelerate recovery of atrophied skeletal muscle. However, convincing stimulation parameters for suppressing muscle atrophy due to injured sciatic nerve remains unclear. The objective of this study was to investigate the effective intensity of MES on restraining muscle atrophy with rat model underwent sciatic nerve injury(SNI). Twenty-5-week-old Sprague Dawley male rats were equally assigned to five groups : Control group(Control, CON, n = 4), Denervation group(Denervation, D, n = 4), Denervation with MES of 22 μA group(Denervation + 22 μA, D+22, n = 4), Denervation with MES of 100 μA group (Denervation + 100 μA, D+100 n = 4), Denervation with MES of 400 μA group(Denervation + 400 μA, D+400, n = 4). To induce muscle atrophy, all rats in the D, D+22, D+100, and D+400 groups, were subjected to sciatic nerve injury on their right hindlimb and allowed to have 1 week of resting period. Following this period, rats underwent daily MES(60 min/ a day, 5times/1week) for 4 weeks. After that, we investigate morphological changes in muscle volume by using in vivo micro-computed tomography at week 0, 1, 3 and 5. After 5 weeks, the muscle volume had the highest value in D+400 group, and also noticeably increased in D+100 group compared to it in D group. The results of this study imply that MES with current intensities between 100-400 μA can suppress muscle atrophy effectively.

참고문헌 (36)

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