조직 재생을 위한 Poly (D, L-lactide-co-glycolide) 표면에 (1→3)(1→6)-ßglucan 고정에 대한 세포 점착 및 성장 효과
Surface Immobilization of ß-(1→3)(1→6)-glucan onto Biodegradable Polymer for Tissue Regeneration
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Abstract
We examined the effects of -glucan-reinforced PLGA film and scaffold on HDFs (human dermal fibroblast) attachment and proliferation. The PLGA films were prepared by simple solvent-casting method. The prepared films were grafted with (1→3)(1→6)--glucan in various ratios after plasma treatment on surface. The surface of the film was characterized by contact angle measurement, scanning electron microscope (SEM), and Fourier-transform infrared spectrophotometer (FT-IR). The amount of (1→3)(1→6)--glucan in the prepared film was indirectly determined by phenol-sulfuric acid method. The HDFs (Human dermal fibroblasts) were used to evaluate the cell attachment and proliferation on PLGA specimens before and after plasma/-glucan treatment. The result showed that the plasma treated groups exhibited more amount of -glucan might be grafted than the non plasma treated groups. Cell attachment was significantly enhanced in the plasma/-glucan grafted group after 4 hours incubation (p<0.05) due to the improved hydrophilicity and cytoactivity effect of the -glucan. The cell proliferation of plasma/-glucan (2mg/ml) grafted group was the highest rate among the groups (p<0.05).
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