수술현미경용 다중형광 관측 시스템 연구
Development of Indocyanine Green and 5-Aminolevulinic Acid Detection System for Surgical Microscope
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Indocyanine green(ICG) and 5-aminolevulinic acid(5-ALA) have been widely used to mark blood vesselsor tumors. However, fluorescent dye detection systems were designed to use one type of dyes only. In this study,we proposed a detection system capable of detecting Indocyanine green and 5-aminolevulinic acid. Multiple filtersand light sources are integrated into a single system. In this study, we performed analysis of fluorescent dyes andconfigured a detection system. During the analysis, it was found that Indocyanine green and 5-aminolevulinic acidhave the maximum intensity at 40 μM. We designed light source for fluorescent dyes and conducted compatibilitytest using a commercial surgical microscope. The fluorescent dye detection system was configured based on theexperimental results. The developed system successfully detects Indocyanine green and 5-aminolevulinic acid. Therefore, more efficient surgical operations can be achieved using both fluorescent dyes at the same time. Weexpect that the developed system can increase the survival rate of patients.
Abstract
Indocyanine green(ICG) and 5-aminolevulinic acid(5-ALA) have been widely used to mark blood vesselsor tumors. However, fluorescent dye detection systems were designed to use one type of dyes only. In this study,we proposed a detection system capable of detecting Indocyanine green and 5-aminolevulinic acid. Multiple filtersand light sources are integrated into a single system. In this study, we performed analysis of fluorescent dyes andconfigured a detection system. During the analysis, it was found that Indocyanine green and 5-aminolevulinic acidhave the maximum intensity at 40 μM. We designed light source for fluorescent dyes and conducted compatibilitytest using a commercial surgical microscope. The fluorescent dye detection system was configured based on theexperimental results. The developed system successfully detects Indocyanine green and 5-aminolevulinic acid. Therefore, more efficient surgical operations can be achieved using both fluorescent dyes at the same time. Weexpect that the developed system can increase the survival rate of patients.
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