Effect of PVA Concentration on Strength and Cell Growth Behavior of PVA/gelatin Hydrogels for Wound Dressing
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Polyvinyl alcohol (PVA)/gelatin hydrogels were prepared by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the strength and the cell growth behavior of the PVA/gelatin hydrogels. The swelling rate of the PVA/gelatin hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel. No appreciable degradation of the hydrogels was detected. As the PVA content increased from 6 wt% to 12 wt%, the strength of the PVA/gelatin hydrogels increased drastically from 6.4±0.9 kPa to 46.6±9.0 kPa. The PVA/gelatin hydrogels did not show any evidence of causing cell lysis or toxicity, implying that the hydrogels are clinically safe and effective. Although the strength increased with increasing the PVA content, the PVA/gelatin hydrogels containing 8 wt% exhibited the fastest cell growth, which is highly suitable for wound dressing requiring fast healing regeneration.
Abstract
Polyvinyl alcohol (PVA)/gelatin hydrogels were prepared by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the strength and the cell growth behavior of the PVA/gelatin hydrogels. The swelling rate of the PVA/gelatin hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel. No appreciable degradation of the hydrogels was detected. As the PVA content increased from 6 wt% to 12 wt%, the strength of the PVA/gelatin hydrogels increased drastically from 6.4±0.9 kPa to 46.6±9.0 kPa. The PVA/gelatin hydrogels did not show any evidence of causing cell lysis or toxicity, implying that the hydrogels are clinically safe and effective. Although the strength increased with increasing the PVA content, the PVA/gelatin hydrogels containing 8 wt% exhibited the fastest cell growth, which is highly suitable for wound dressing requiring fast healing regeneration.
참고문헌 (18)
- 한승규, "우리나라의 최신 창상피복법", 대한의사협회지, 54(6), pp.594~603 (2011), 대한의사협회
- 김진, "알로인이 함유된 창상치료제용 PVA", CMC 하이드로젤의 개발, 2013, pp.37(6), 802~808[DOI]
- Hassan CM, "Structure and morphology of freeze", thawed PVA hydrogels, 2000, pp.33(7), 2472~2479
- 조선영, "동결", 융해와 방사선 가교법에 의한 PVA, 폴리머, pp.2009, 33(6)[DOI]
- 신지연, "Synthesis and Biocompatibility of PVA", NaCMC Hydrogels Crosslinked by Cyclic Freezing, 의공학회지, pp.2018, 39(4)[DOI]
- Hwang S, "Characterization and preparation of the hydrogel has excellent release effect of the active ingredients using a radiation crosslinking technology", J. Radiat. Industry, 9(2), pp.199~207 (2015)
- Lim KS, "Covalent incorporation of non-chemically modified gelatin into degradable PVA-tyramine hydrogels", Biomater, 34(29), pp.7097~7105 (2013)
- Wang F, "Structure and properties of bone-like-nanohydroxyapatite", gelatin, Adv. Biosci. Biotechnol, pp.2010, 1(3)[DOI]
- Mahnama H, "Preparation of biodegradable gelatin", PVA porous scaffolds for skin regeneration, 2017, pp.45(5), 928~935
- 김진태, "Tissue Response to Implants of Hyaluronic Acid Hydrogel Prepared by Microbeads", 조직공학과 재생의학, 11(1), pp.32~38 (2014), 한국조직공학과 재생의학회[DOI]
- 이득용, "Influence of Molecular Weight on Swelling and Elastic Modulus of Hyaluronic Acid Dermal Fillers", 폴리머, 39(6), pp.976~980 (2015), 한국고분자학회[DOI]
- 전철병, "Effect of molecular weight of hyaluronic acid (HA) on viscoelasticity and particle texturing feel of HA dermal biphasic fillers", 생체재료학회지, 20(4), pp.275~281 (2016), 한국생체재료학회[DOI]
- 설보경, "전기방사법으로 제조한 PU", PEG 복합 지지체의 특성, 2017, pp.38(5), 248~255[DOI]
- 오가연, "Synthesis and Characterization of Electrospun PU", PCL Hybrid Scaffolds, 2018, pp.26(1), 48~53[DOI]
- 김예나, "Effect of PEG Addition on Pore Morphology and Biocompatibility of PLLA Scaffolds Prepared by Freeze Drying", Biomedical Engineering Letters (BMEL), 6(4), pp.287~295 (2016), 대한의용생체공학회[DOI]
- Torelli GF, "A good manufacturing practice method to ex vivo expand natural killer cells for clinical use", Blood Tansfus, 13(3), pp.464~471 (2015)
- 조덕, "Expansion and Activation of Natural Killer Cells for Cancer Immunotherapy", Annals of Laboratory Medicine, 29(2), pp.89~96 (2009), 대한진단검사의학회
- Santos SC, "Photopolymerizable platelet lysate hydrogels for customizable 3D cell culture platforms", Adv. Healthcare Mater, pp.1800849~ (2018)