Comparison of Three Commercially Available Assays for Measurement of Vitamin D
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Background: We evaluated three commercially available vitamin D assays to evaluate and compare the correlation and accuracy among them. Methods: Vitamin D was measured in 71 patient samples using the Architect 25-OH vitamin D assay (Abbott), the ADVIA Centaur vitamin D total assay (Siemens), and the LIAISON 25 OH vitamin D total assay (Diasorin). The evaluation made use of both patient samples and standard reference material, SRM 972. To analyze correlations and differences, Pearson’s correlation coefficients and paired sample t-tests were performed. Results: Correlations among the three evaluated assays showed strong positive linear relationships (correlations among Siemens and DiaSorin, DiaSorin and Abbott, Abbott and Siemens: r=0.935, r=0.927, r=0.909, respectively). Mean (SD) vitamin D values on Siemens, Abbott, and DiaSorin assays in the 71 patient samples were 23.09 (10.41), 16.75 (11.26), and 16.76 (9.32), respectively. Results for the Siemens assay were significantly different from the other two methods (P<0.001). Target values for SRM 972 level 1, 2, 3, and 4 were 23.9, 14.0, 44.9, and 35.4, respectively. The Abbott, Siemens, and Diasorin assay values were closest to the target values in level 1, levels 2 and 3, and level 4, respectively. Conclusions: Correlations among vitamin D assays were good; however, the mean values of the Siemens assay were significantly higher than those of DiaSorin or Abbott. We found significant differences in vitamin D levels and discrepancies between patient samples and SRM 972 samples, which should be considered during use in a clinical setting.
Abstract
Background: We evaluated three commercially available vitamin D assays to evaluate and compare the correlation and accuracy among them. Methods: Vitamin D was measured in 71 patient samples using the Architect 25-OH vitamin D assay (Abbott), the ADVIA Centaur vitamin D total assay (Siemens), and the LIAISON 25 OH vitamin D total assay (Diasorin). The evaluation made use of both patient samples and standard reference material, SRM 972. To analyze correlations and differences, Pearson’s correlation coefficients and paired sample t-tests were performed. Results: Correlations among the three evaluated assays showed strong positive linear relationships (correlations among Siemens and DiaSorin, DiaSorin and Abbott, Abbott and Siemens: r=0.935, r=0.927, r=0.909, respectively). Mean (SD) vitamin D values on Siemens, Abbott, and DiaSorin assays in the 71 patient samples were 23.09 (10.41), 16.75 (11.26), and 16.76 (9.32), respectively. Results for the Siemens assay were significantly different from the other two methods (P<0.001). Target values for SRM 972 level 1, 2, 3, and 4 were 23.9, 14.0, 44.9, and 35.4, respectively. The Abbott, Siemens, and Diasorin assay values were closest to the target values in level 1, levels 2 and 3, and level 4, respectively. Conclusions: Correlations among vitamin D assays were good; however, the mean values of the Siemens assay were significantly higher than those of DiaSorin or Abbott. We found significant differences in vitamin D levels and discrepancies between patient samples and SRM 972 samples, which should be considered during use in a clinical setting.
참고문헌 (37)
- Audran M, "The physiology and pathophysiology of vitamin D", Mayo Clin Proc, 60, pp.851~866 (1985)
- Chlebowski RT, "Vitamin D and breast cancer : interpreting current evidence", Breast Cancer Res, 13, pp.217~ (2011)
- Chiang KC, "Hepatocellular carcinoma and vitamin D : a review", J Gastroenterol Hepatol, 26, pp.1597~1603 (2011)
- Woloszynska-Read A, "Vitamin D and cancer : clinical aspects", Best Pract Res Clin Endocrinol Metab, 25, pp.605~615 (2011)
- Choi HS, "Low serum vitamin D is associated with high risk of diabetes in Korean adults", J Nutr, 141, pp.1524~1528 (2011)
- Holick MF, "Vitamin D de ciency", N Engl J Med, 357, pp.266~281 (2007)
- Nair R, "Vitamin D : The"sunshine"vitamin", J Pharmacol Pharmacother, 3, pp.118~126 (2012)
- DeLuca HF, "Overview of general physiologic features and functions of vitamin D", Am J Clin Nutr, 80, pp.1689S~1696S (2004)
- Dawson-Hughes B, "Estimates of optimal vitamin D status", Osteoporos Int, 16, pp.713~716 (2005)
- Holick MF, "Vitamin D status: measurement, interpretation, and clinical application", Ann Epidemiol, 19, pp.73~78 (2009)
- Hsu JJ, "Vitamin D and osteogenic differentiation in the artery wall", Clin J Am Soc Nephrol, 3, pp.1542~1547 (2008)
- Medici D, "FGF-23-Klotho signaling stimulates proliferation and prevents vitamin D-induced apoptosis", J Cell Biol, 182, pp.459~465 (2008)
- Bischoff-Ferrari HA, "Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes", Am J Clin Nutr, 84, pp.18~28 (2006)
- Carter GD, "How accurate are assays for 25-hydroxyvitamin D? Data from the international vitamin D external quality assessment scheme", Clin Chem, 50, pp.2195~2197 (2004)
- Farrell C, "25-Hydroxyvitamin D testing : challenging the performance of current automated immunoassays", Clin Chem Lab Med, 50, pp.1953~1963 (2012)
- Heaney RP, "Vitamin D(3) is more potent than vitamin D(2) in humans", J Clin Endocrinol Metab, 96, pp.E447~E452 (2011)
- Holick MF, "Vitamin D2 is as effective as vitamin D3 in maintaining circulating concentrations of 25-hydroxyvitamin D", J Clin Endocrinol Metab, 93, pp.677~681 (2008)
- Yu S, "25OHD analogues and vacuum blood collection tubes dramatically affect the accuracy of automated immunoassays", Sci Rep, 5, pp.14636~ (2015)
- Heijboer AC, "Accuracy of 6 routine 25-hydroxyvitamin D assays : in uence of vitamin D binding protein concentration", Clin Chem, 58, pp.543~548 (2012)
- 이준형, "Discrepancy between Vitamin D Total Immunoassays due to Various Cross-reactivities", 대한골대사학회지, 22(3), pp.107~112 (2015), 대한골대사학회[DOI]
- Enko D, "25-hydroxy-Vitamin D status : limitations in comparison and clinical interpretation of serum-levels across different assay methods", Clin Lab, 60, pp.1541~1550 (2014)
- Wallace AM, "Measurement of 25-hydroxyvitamin D in the clinical laboratory: current procedures, performance characteristics and limitations", Steroids, 75, pp.477~488 (2010)
- (2010), Abbott Labratories
- (2013), Siemens Healthcare Diagnostics Inc.
- Freeman J, "In uence of vitamin D binding protein on accuracy of 25-hydroxyvitamin D measurement using the ADVIA Centaur Vitamin D Total Assay", Int J Endocrinol, 2014, pp.691679~ (2014)
- Sinotte M, "Genetic polymorphisms of the vitamin D binding protein and plasma concentrations of 25-hydroxyvitamin D in premenopausal women", Am J Clin Nutr, 89, pp.634~640 (2009)
- Kamboh MI, "Ethnic variation in vitamin D-binding protein(GC) : a review of isoelectric focusing studies in human populations", Hum Genet, 72, pp.281~293 (1986)
- Santos BR, "Variations in the vitamin D-binding protein(DBP)gene are related to lower 25-hydroxyvitamin D levels in healthy girls : a cross-sectional study", Horm Res Paediatr, 79, pp.162~168 (2013)
- Park Y, "Relationship between vitamin D-binding protein polymorphisms and blood vitamin D level in Korean patients with COPD", Int J Chron Obstruct Pulmon Dis, 11, pp.731~738 (2016)
- Moon HW, "Comparison of four current 25-hydroxyvitamin D assays", Clin Biochem, 45, pp.326~330 (2012)
- Wyness SP, "Performance characteristics of six automated 25-hydroxyvitamin D assays : Mind your 3s and 2s", Clin Biochem, 48, pp.1089~1096 (2015)
- Li L, "Performance evaluation of two immunoassays for 25-hydroxyvitamin D", J Clin Biochem Nutr, 58, pp.186~192 (2016)
- Hsu SA, "Evaluation of two automated immunoassays for 25-OH vitamin D : comparison against LC-MS", MS, 2013, pp.136, 139~145
- Chen Y, "Performance evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D assays", Clin Biochem, 45, pp.1485~1490 (2012)
- Saenger AK, "Quantification of serum 25-hydroxyvitamin D(2)and D(3)using HPLC-tandem mass spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency", Am J Clin Pathol, 125, pp.914~920 (2006)
- Bikle DD, "Free 1,25-dihydroxyvitamin D levels in serum from normal subjects, pregnant subjects, and subjects with liver disease", J Clin Invest, 74, pp.1966~1971 (1984)
- Harris SS, "Seasonal changes in plasma 25-hydroxyvitamin D concentrations of young Ame women", Am J Clin Nutr, 67, pp.1232~1236 (1998)