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Cutaneous Photorejuvenation of Light Emitting Diodes via the Melatonin Membrane Receptor Pathway

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초록

Background: Melatonin receptors are present in the human skin and retina. These receptorscan be stimulated by light emitting diodes (LEDs) at specific wavelengths, thereby inducingcutaneous photorejuvenation. However, the underlying mechanism remains unclear. Objective: To evaluate the inf luence of LEDs at specific wavelengths on melatonin mem-brane receptor (MT1) and cutaneous photorejuvenation via the MT1 pathway in vitro. Methods: Normal human dermal fibroblasts (HDFs) were irradiated using LEDs at differentwavelengths (410~940 nm) at a dose of 1 J/cm2. MT1 activity was evaluated after melatoninstimulation and LED irradiation. Thereafter, the expressions of collagen (COL) and matrixmetalloproteinases (MMPs), with and without luzindole (MT1/2 receptor antagonist), wereinvestigated via semi-quantitative reverse transcription polymerase chain reaction (PCR),real-time PCR, western blotting, and enzyme-linked immunosorbent assay. Results: In HDFs, the MT1 mRNA and protein levels increased significantly in response tomelatonin (dose, 50 nM) (p<0.01) and LED irradiation at 595, 630, 850, and 940 nm (p<0.01). LED irradiation up-regulated COL type I and down-regulated MMP-1. Compared to LEDirradiation without luzindole, LED irradiation with luzindole produced no significant in-crease in COL type I mRNA and protein levels (p< 0 . 01). Conclusion: We found that LED irradiation induces collagen synthesis and MMP-1 inhibi-tion in HDFs via MT1 activation. Additionally, multiple LED wavelengths (595, 630, 850,and 940 nm) stimulated MT1 in HDFs, unlike in the eyes, where only blue light inducedplasma melatonin suppression. This suggests the possibility of the melatoninergic pathwayin photorejuvenation.

Abstract

Background: Melatonin receptors are present in the human skin and retina. These receptorscan be stimulated by light emitting diodes (LEDs) at specific wavelengths, thereby inducingcutaneous photorejuvenation. However, the underlying mechanism remains unclear. Objective: To evaluate the inf luence of LEDs at specific wavelengths on melatonin mem-brane receptor (MT1) and cutaneous photorejuvenation via the MT1 pathway in vitro. Methods: Normal human dermal fibroblasts (HDFs) were irradiated using LEDs at differentwavelengths (410~940 nm) at a dose of 1 J/cm2. MT1 activity was evaluated after melatoninstimulation and LED irradiation. Thereafter, the expressions of collagen (COL) and matrixmetalloproteinases (MMPs), with and without luzindole (MT1/2 receptor antagonist), wereinvestigated via semi-quantitative reverse transcription polymerase chain reaction (PCR),real-time PCR, western blotting, and enzyme-linked immunosorbent assay. Results: In HDFs, the MT1 mRNA and protein levels increased significantly in response tomelatonin (dose, 50 nM) (p<0.01) and LED irradiation at 595, 630, 850, and 940 nm (p<0.01). LED irradiation up-regulated COL type I and down-regulated MMP-1. Compared to LEDirradiation without luzindole, LED irradiation with luzindole produced no significant in-crease in COL type I mRNA and protein levels (p< 0 . 01). Conclusion: We found that LED irradiation induces collagen synthesis and MMP-1 inhibi-tion in HDFs via MT1 activation. Additionally, multiple LED wavelengths (595, 630, 850,and 940 nm) stimulated MT1 in HDFs, unlike in the eyes, where only blue light inducedplasma melatonin suppression. This suggests the possibility of the melatoninergic pathwayin photorejuvenation.

참고문헌 (38)

  1. Arendt J, "Melatonin and human rhythms", Chronobiol Int, 23, pp.21~37 (2006)
  2. Tan DX, "Chemical and physical properties and potential mechanisms : melatonin as a broad spectrum antioxidant and free radical scavenger", Curr Top Med Chem, 2, pp.181~197 (2002)
  3. Fischer TW, "Oxidants and antioxidants in cutaneous biology: current problems in dermatology", pp.165~174 (2001), Karger
  4. Guerrero JM, "Melatonin-immune system relationships", Curr Top Med Chem, 2, pp.167~179 (2002)
  5. Mediavilla MD, "Basic mechanisms involved in the anti-cancer effects of melatonin", Curr Med Chem, 17, pp.4462~4481 (2010)
  6. Claustrat B, "Melatonin and jet lag : confirmatory result using a simplified protocol", Biol Psychiatry, 32, pp.705~711 (1992)
  7. Tajes M, "Anti-aging properties of melatonin in an in vitro murine senescence model : involvement of the sirtuin 1 pathway", J Pineal Res, 47, pp.228~237 (2009)
  8. Kyu-SukLee, "Melatonin reduces ultraviolet-B induced cell damages and polyamine levels in human skin fibroblasts in culture", Experimental and Molecular Medicine, 35(4), pp.263~268 (2003), 생화학분자생물학회
  9. Pugazhenthi K, "Melatonin accelerates the process of wound repair in full-thickness incisional wounds", J Pineal Res, 44, pp.387~396 (2008)
  10. Kvetnoy I, "Extrapineal melatonin in pathology: new perspectives for diagnosis, prognosis and treatment of illness", Neuro Endocrinol Lett, 23(1), pp.92~96 (2002)
  11. Pandi-Perumal SR, "Physiological effects of melatonin : role of melatonin receptors and signal transduction pathways", Prog Neurobiol, 85, pp.335~353 (2008)
  12. Reiter RJ, "Medical implications of melatonin : receptor-mediated and receptor-independent actions", Adv Med Sci, 52, pp.11~28 (2007)
  13. Sugden D, "Melatonin, melatonin receptors and melanophores : a moving story", Pigment Cell Res, 17, pp.454~460 (2004)
  14. Slominski A, "Functional activity of serotoninergic and melatoninergic systems expressed in the skin", J Cell Physiol, 196, pp.144~153 (2003)
  15. Brainard GC, "Light and biological rhythms in man", pp.29~53 (1993), Pergamon
  16. Thapan K, "An action spectrum for melatonin suppression : evidence for a novel non-rod, non-cone photoreceptor system in humans", J Physiol, 535(Pt 1), pp.261~267 (2001)
  17. Brainard GC, "Effect of light wavelength on the suppression of nocturnal plasma melatonin in normal volunteers", Ann N Y Acad Sci, 453, pp.376~378 (1985)
  18. Brainard GC, "Action spectrum for melatonin regulation in humans : evidence for a novel circadian photoreceptor", J Neurosci, 21, pp.6405~6412 (2001)
  19. Shin HS, "Effects of LED light spectra on oxidative stress and the protective role of melatonin in relation to the daily rhythm of the yellowtail clownfish, Amphiprion clarkii", Comp Biochem Physiol A Mol Integr Physiol, 160, pp.221~228 (2011)
  20. Bolton P, "The direct effect of 860 nm light on cell proliferation and on succinic dehydrogenase activity of human fibroblasts in vitro", Laser Ther, 7, pp.55~60 (1995)
  21. Lee SY, "A prospective, randomized, placebo-controlled, double-blinded, and split-face clinical study on LED phototherapy for skin rejuvenation : clinical, profilometric, histologic, ultrastructural, and biochemical evaluations and comparison of three different treatment settings", J Photochem Photobiol B, 88, pp.51~67 (2007)
  22. Zhang Y, "cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light", J Invest Dermatol, 120, pp.849~857 (2003)
  23. de Freitas LF, "Proposed mechanisms of photobiomodulation or low-level light therapy", IEEE J Sel Top Quantum Electron, 22, pp.7000417~ (2016)
  24. Fischer TW, "Melatonin as a major skin protectant : from free radical scavenging to DNA damage repair", Exp Dermatol, 17, pp.713~730 (2008)
  25. Eşrefoğlu M, "Potent therapeutic effect of melatonin on aging skin in pinealectomized rats", J Pineal Res, 39, pp.231~237 (2005)
  26. Choi JY, "Propionibacterium acnes stimulates pro-matrix metalloproteinase-2 expression through tumor necrosis factor-alpha in human dermal fibroblasts", J Invest Dermatol, 128, pp.846~854 (2008)
  27. Fisher GJ, "Looking older : fibroblast collapse and therapeutic implications", Arch Dermatol, 144, pp.666~672 (2008)
  28. Weiss RA, "Clinical trial of a novel non-thermal LED array for reversal of photoaging : clinical, histologic, and surface profilometric results", Lasers Surg Med, 36, pp.85~91 (2005)
  29. Baez F, "The use of light-emitting diode therapy in the treatment of photoaged skin", J Cosmet Dermatol, 6, pp.189~194 (2007)
  30. Illescas-Montes R, "Cultured human fibroblast biostimulation using a 940 nm diode laser", Materials(Basel), 10, pp.793~ (2017)
  31. Farivar S, "Biological effects of low level laser therapy", J Lasers Med Sci, 5, pp.58~62 (2014)
  32. Liu H, "Redox-dependent transcriptional regulation", Circ Res, 97, pp.967~974 (2005)
  33. Hamblin MR, "Mechanisms of low level light therapy" (2006), Proceedings SPIE
  34. Joensen J, "An experimental study of low-level laser therapy in rat Achilles tendon injury", Lasers Med Sci, 27, pp.103~111 (2012)
  35. Hosseinzadeh A, "Apoptosis signaling pathways in osteoarthritis and possible protective role of melatonin", J Pineal Res, 61, pp.411~425 (2016)
  36. Griefahn B, "Effects of electromagnetic radiation(bright light, extremely lowfrequency magnetic fields, infrared radiation)on the circadian rhythm of melatonin synthesis, rectal temperature, and heart rate", Ind Health, 40, pp.320~327 (2002)
  37. Gooley JJ, "Melanopsin in cells of origin of the retinohypothalamic tract", Nat Neurosci, 4, pp.1165~ (2001)
  38. Reppert SM, "Molecular characterization of a second melatonin receptor expressed in human retina and brain : the Mel1b melatonin receptor", Proc Natl Acad Sci U S A, 92, pp.8734~8738 (1995)

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