Various Wavelengths of Light-Emitting Diode Light Regulate the Proliferation of Human Dermal Papilla Cells and Hair Follicles via Wnt/β- Catenin and the Extracellular Signal-Regulated Kinase Pathways
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Background: The human dermal papilla cells (hDPCs) play an important role in regulation of hair cycling and growth. Objective: The aim of this study was to investigate the effect of different wavelengths of light-emitting diode (LED) irradiation on the proliferation of cultured hDPCs and on the growth of human hair follicles (HFs) in vitro. Methods: We examined the effect of LED irradiation on Wnt/β-catenin signaling and mitogen-activated protein kinase (MAPK) pathways in hDPCs. Anagen HFs were cultured with LED irradiation and elongation of each hair shaft was measured. Results: The most potent wavelength in promoting the hDPC proliferation is 660 nm and 830 nm promoted hDPC proliferation to a lesser extent than 660 nm. Various wavelengths significantly increased β-catenin, Axin2, Wnt3a, Wnt5a and Wnt10b mRNA expression. LED irradiation significantly increased β-catenin and cyclin D expression, and the phosphorylation of MAPK and extracellular signal-regulated kinase (ERK). HFs irradiated with 415 nm and 660 nm grew longer than control. Conclusion: Our result suggests that LED has a potential to stimulate hDPC proliferation via the activation of Wnt/β-catenin signaling and ERK pathway. To our best knowledge, this is the first report which investigated that the effect of various wavelengths of LED on hDPC proliferation and the underlying mechanisms.
Abstract
Background: The human dermal papilla cells (hDPCs) play an important role in regulation of hair cycling and growth. Objective: The aim of this study was to investigate the effect of different wavelengths of light-emitting diode (LED) irradiation on the proliferation of cultured hDPCs and on the growth of human hair follicles (HFs) in vitro. Methods: We examined the effect of LED irradiation on Wnt/β-catenin signaling and mitogen-activated protein kinase (MAPK) pathways in hDPCs. Anagen HFs were cultured with LED irradiation and elongation of each hair shaft was measured. Results: The most potent wavelength in promoting the hDPC proliferation is 660 nm and 830 nm promoted hDPC proliferation to a lesser extent than 660 nm. Various wavelengths significantly increased β-catenin, Axin2, Wnt3a, Wnt5a and Wnt10b mRNA expression. LED irradiation significantly increased β-catenin and cyclin D expression, and the phosphorylation of MAPK and extracellular signal-regulated kinase (ERK). HFs irradiated with 415 nm and 660 nm grew longer than control. Conclusion: Our result suggests that LED has a potential to stimulate hDPC proliferation via the activation of Wnt/β-catenin signaling and ERK pathway. To our best knowledge, this is the first report which investigated that the effect of various wavelengths of LED on hDPC proliferation and the underlying mechanisms.
참고문헌 (17)
- Mester E, "The effect of laser beams on the growth of hair in mice", Radiobiol Radiother (Berl), 9, pp.621~626 (1968)
- Avci P, "Low-level laser (light) therapy (LLLT) for treatment of hair loss", Lasers Surg Med, 46, pp.144~151 (2014)
- 이원주, "Efficacy of Red or Infrared Light-Emitting Diodes in a Mouse Model of Propionibacterium acnes-Induced Inflammation", Annals of Dermatology, 28(2), pp.186~191 (2016), 대한피부과학회
- Wikramanayake TC, "Effects of the Lexington LaserComb on hair regrowth in the C3H", HeJ mouse model of alopecia areata, 2012, pp.27, 431~436
- King LE Jr, "Lack of response to laser comb in spontaneous and graft-induced alopecia areata in C3H", HeJ mice, 2014, pp.134, 264~266
- Messenger AG, "The in vitro properties of dermal papilla cell lines established from human hair follicles", Br J Dermatol, 114, pp.425~430 (1986)
- Twentyman PR, "A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity", Br J Cancer, 56, pp.279~285 (1987)
- Philpott MP, "Human hair growth in vitro", J Cell Sci, 97, pp.463~471 (1990)
- Kishimoto J, "Wnt signaling maintains the hair-inducing activity of the dermal papilla", Genes Dev, 14, pp.1181~1185 (2000)
- Sheen YS, "Visible red light enhances physiological anagen entry in vivo and has direct and indirect stimulative effects in vitro", Lasers Surg Med, 47, pp.50~59 (2015)
- Simpson CR, "Near-infrared optical properties of ex vivo human skin and subcutaneous tissues measured using the Monte Carlo inversion technique", Phys Med Biol, 43, pp.2465~2478 (1998)
- Weiss RA, "Clinical trial of a novel non-thermal LED array for reversal of photoaging: clinical, histologic, and surface profilometric results", Lasers Surg Med, 36, pp.85~91 (2005)
- Shimizu H, "Wnt signaling through the betacatenin pathway is sufficient to maintain, but not restore, anagen-phase characteristics of dermal papilla cells", J Invest Dermatol, 122, pp.239~245 (2004)
- Ouji Y, "Canonical Wnts, specifically Wnt-10b, show ability to maintain dermal papilla cells", Biochem Biophys Res Commun, 438, pp.493~499 (2013)
- Shefer G, "Skeletal muscle cell activation by low-energy laser irradiation: a role for the MAPK", ERK pathway, 2001, pp.187, 73~80
- Dent P, "MAPK pathways in radiation responses", Oncogene, 22, pp.5885~5896 (2003)
- Botchkarev VA, "Molecular control of epithelial-mesenchymal interactions during hair follicle cycling", J Investig Dermatol Symp Proc, 8, pp.46~55 (2003)