식물생장조절물질, 무기물 농도 및 질소원 비율이 Gymmema sylvestre 세포 배양에 미치는 영향
Effects of Plant Growth Regulators, Medium Salt Strength and Nitrogen Ratio on Cell Culture of Gymmema sylvestre
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
This study was carried out to investigate the effects of plant growth regulators, medium salt strength and nitrogen ratio on cell culture of Gymnema sylvestre. Cell growth was inhibited by 2,4-D concentrations higher than 1.0 mg L-1, but not by kinetin concentrations lower than 0.5 mg L-1. Maximal cell growth was obtained at 1.0 mg L-1 2,4-D and 0.1 mg L-1 kinetin. Cell growth was greatest at 1x MS medium but high strength MS medium inhibited cell growth due to low water potential in the medium. In NH4+:NO3- ratio of 0:60 (ie. 0.0 mM NH4+ and 60 mM NO3-), cells growth was highest but cells were smaller and whiter compared with those in other NH4+:NO3- ratio. Reduced cell growth was observed with continuous culture. These results suggested that optimal culture of Gymnema sylvestre could be achieved with 1x MS medium with 20:40 ratio of NH4+:NO3- supplemented with 1.0 mg L-1 2,4-D and 0.1 mg L-1 kinetin.