갯방풍(Glehnia littoralis Schmidt et Miquiel)에서 캘러스 유도 및 식물체 재분화에 미치는 생장조절제의 효과
Comparative Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Glehnia littoralis Schmidt et Miquiel
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Abstract
Glehnia littoralisis known as an edible and medicinal plant using green leaves and matureroots of plant. In the present paper, the influence of plant growth regulators on callusinduction and plant regeneration was investigated. Callus induction and regenerationoccurred from leaf and petiole explants in Glehnia littoralis. Optimal condition of plantgrowth regulators for callus induction from leaf and petiole explants was MS basal mediumsupplemented with 2mg/L 2,4-D and 2mg/L BA. The frequency of callus induction washigher in petiole explant than leaf. When the callus was cultured on MS basal mediumsupplemented with 0~1mg/L IAA, 0~1mg/L NAA and 0~2mg/L BA for about 65 days, themost effective plant growth regulators on plant regeneration from callus were 1mg/L NAAand 2mg/L BA. The plantlets acclimatized successfully and grown in vermiculite matrix.
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