In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata
0
조회
0
다운로드
3
인용
0
참고문헌
초록
Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.
Abstract
Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.
참고문헌 (45)
- Barbara T, "In vitro propagation of isoflavone-producting Pueraria lobata (Willd.) Ohwi", Plant Sci, 165, pp.1123~1128 (2003)
- Bonhomme V, "Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only", J Biotechnol, 8, pp.151~158 (2000)
- Boonsnongcheep P, "Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures", Plant Cell Tiss Cult, 101, pp.119~126 (2010)
- Bousquet J, "DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction", Can J For Res, 20, pp.254~257 (1990)
- Bulgakov VP, "Inhibitory effect of the Agrobacterium rhizogenes rolC gene on rabdosiin and rosmarinic acid production in Eritrichium sericeum and Lithospermum erythrorhizon transformed cell cultures", Plant, 221, pp.471~478 (2005)
- Cardarelli M, "Agrobacterium rhizogenes T-DNA gene capable of inducting hairy root phenotype", Mol Gen Genet, 209, pp.475~480 (1987)
- Chansakaow S, "Isoflavonoids from Pueraria mirifica and their estrogenic activity", Planta Med, 66, pp.572~574 (2000)
- Chen G, "Nutrient consumption and production of isoflavones in bioreactor cultures of Pueraria lobata (Willd)", J Environ Biol, 28, pp.321~326 (2007)
- Cherdshewasart W, "Metabolic activation promotes estrogenic activity of the phytoestrogen-rich plant", Maturitas, 59, pp.128~136 (2008)
- Cherdshewasart W, "Major isoflavonoid contents of the phytoestrogen rich-herb Pueraria mirifica in comparison with Pueraria lobata", J Pharm Biomed Anal, 43, pp.428~434 (2007)
- Crane C, "Transgenic Medicago truncatula plants obtained from Agrobacterium tumefaciens-transformed roots and Agrobacterium rhizogenestransformed hairy roots", Planta, 223, pp.1344~1354 (2006)
- Doyle JJ, "Isolation of plant DNA from fresh tissue", Focus, 12, pp.13~15 (1990)
- Giri A, "Transgenic hairy roots: recent trends and applications", Biotechnol Adv, 18, pp.1~22 (2000)
- Goyal S, "Effect of chemical factors on production of isoflavones in Pueraria tuberose (Roxb.ex. Willd.) DC suspension culture", Indian J Exp Biol, 45, pp.1063~1067 (2007)
- Goyal S, "Increased isoflavonids accumulation in cell suspension cultures of Pueraria tuberose by elicitors", Indian J Biotechnol, 7, pp.378~382 (2008)
- 황성진, "Baicalin Production in Transformed Hairy Root Clones of Scutellaria baicalensis", Biotechnology and Bioprocess Engineering, 11(2), pp.105~109 (2006), 한국생물공학회
- ChungsookKim, "Study of Substance Changes in Flowers of Pueraria thunbergiana Benth. During Storage", Archives of Pharmacal Research, 26(3), pp.210~213 (2003), 대한약학회
- 김동갑, "RAPD분자마커를 이용한 칡(콩과) 및 근연분류군의 유전적 변이 및 유연관계", 한국자원식물학회지, 22(5), pp.446~453 (2009), 한국자원식물학회
- 김종세, "Agrobacterium rhizogenes strains이 배초향 모상근 유도와 Rosmarinic acid 생산에 미치는 영향", 한국자원식물학회지, 23(1), pp.14~18 (2010), 한국자원식물학회
- Kinjo J-E, "Studies on the constituents of Pueraria lobata. III: Isoflavonoids and related compounds in the roots and the voluble stems", Chem Pharm Bull, 35, pp.4846~4850 (1987)
- Kinjo J, "Hepatoprotective and hepatotoxic activities of Soporadiol analogs on rat primary liver cell cultures", Biol Chem Bull, 23, pp.1118~1121 (2000)
- 유장렬, "기능획득 돌연변이 인삼 모상근의 대량생산", Journal of Plant Biotechnology, 34(4), pp.285~291 (2007), 한국식물생명공학회
- Korsangruang S, "Effects of abiotic and biotic elicitors on growth and isoflvonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures", Plant Cell Tiss Organ Cult, 103, pp.333~342 (2010)
- Limpens E, "RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula", J Exp Bot, 55, pp.983~992 (2004)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Plant, 15, pp.473~497 (1962)
- Ono NN, "Establishment of pomegranate (Punica granatum) hairy root cultures for genetic interrogation of the hydrolysable tannin biosynthetic pathway", Planta, 236, pp.931~941 (2012)
- Pandey N, "Antioxidant properties of different fractions of tubers from Pueraria tuberose Linn", Food Chem, 105, pp.219~222 (2007)
- Pappert RA, "Genetic variation in Pueraria lobata (Fabaceae), an introduced, clonal, invasive plant of the southeastern United States", Am J Bot, 87, pp.1240~1245 (2000)
- Park T-H, "A new resistance gene to powdery mildew identified in Solanum neorossii has been localized on the short arm of potato chromosome 6", Euphytica, 166, pp.331~339 (2009)
- Prasain JK, "Identification of isoflavones glycosides in Puerarialobata cultures by tandem mass spectrometry", PhytochemAnal, 18, pp.50~59 (2007)
- Rathore MS, "Micropropagation of Pueraria tuberose (Roxb. Ex Willd.) and determination of puerarin content in different tissues", Plant Cell Tiss Organ Cult, 99, pp.327~334 (2009)
- Reppert A, "Isolation of radiolabeled isoflavones from Kudzu (Pueraria lobata) root cultures", J Agric. Food Chem, 56, pp.7860~7865 (2008)
- Santos-Gomes PC, "Phenolic antioxidant compounds produced by in vitro shoots of sage (Salvia officinalis L.)", Plant Sci, 162, pp.981~987 (2002)
- Sato T, "Mechanism of antioxidant action of pueraria glycoside (PG)-1 (an isoflavonoid) and mangiferin (a xanthonoid)", Chem Pharm Bull, 40, pp.721~724 (1992)
- Sharma V, "Scale up production of isoflavonoids in cell suspension cultures of Pueraria tuberose grown in shake flasks and bioreactor", Eng Life Sci, 9, pp.267~271 (2009)
- Sharma V, "Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberose grown in growtek bioreactor with aeration", Physiol Mol Biol Plants, 17, pp.87~92 (2011)
- Shi H.-P, "Genetic transformation of Pueraria phaseoloides with Agrobacterium rhizogenes and puerarin production in hairy roots", Plant Cell Rep, 21, pp.1103~1107 (2003)
- Swain SS, "Hairy root cultures of butterfly pea (Clitoria ternatea L.): Agrobacterium × plant factors influencing transformation", World J Microbiol Biotechnol, 28, pp.729~739 (2012)
- Thanonkeo S, "Production of isoflavones, daidzein and genistein in callus cultures of Pueraria candollei Wall. Ex Benth. Var. mirifica", Songklanakarin J Sci Technol, 28, pp.45~53 (2006)
- Thiem B, "In vitro propagation of isolavone-producing Pueraria lobata (Willd.) Ohwi", Plant Sci, 165, pp.1123~1128 (2003)
- 박상언, "Herbicidal activity of phenolic compounds from hairy root cultures of Fagopyrum tataricum", WEED RESEARCH, 52(1), pp.25~33, WILEY-BLACKWELL
- Udomsuk L, "Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation", Biotechnol Lett, 33, pp.369~374 (2011)
- Vaishnav K, "Isoflavonoids production in callus culture of Pueraria tuberose, the Indian kudzu", Indian J Exp Biol, 44, pp.1012~1017 (2006)
- Verma P, "In vitro-studies in Plumbago zeylanica: rapid micropropagation and establishment of higher plumbagin yielding hairy root cultures", J Plant Physiol, 159, pp.547~552 (2002)
- Villalobos-Amador E, "Organogenesis and Agrobacterium rhizogenesinduced rooting in Pinus maximarinezii Rzedowsky and P. pinceana Gordon", Plant Cell Rep, 20, pp.779~785 (2002)