뚜껑덩굴 부정근 배양에 암조건 및 광조건 처리가 대사물 변화와 항산화 활성에 미치는 효과
Impact of Dark and Light Treatment on Metabolic Changes and Antioxidant Activities in Adventitious Root Culture of Actinostemma lobatum
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Background: Tissue culture is an important strategy for metabolite accumulation in plants. More- over, adventitious roots can produce high levels of phytochemicals. Actinostemma lobatum is con- sumed as a medicinal plant in South Korea, China, India, and Thailand. In this study, we aimed to identify the effects of darkness and light on metabolic changes and antioxidant activity in the adventitious roots of A. lobatum. Methods and Results: To confirm metabolic changes and antioxidant activity, adventitious roots of A. lobatum were grown under appropriate conditions and separated into dark and light treatment groups. Light conditions were found to more significantly influence the accumulation of natural pigments, such as chlorophyll a [0.136 ± 0.001 ㎎·g-1 dry weight (DW)] and total carotenoids (0.047 ± 0.001 ㎎·g-1 DW), than dark conditions (0.072 ± 0.001 ㎎·g-1 DW and 0.026 ± 0.003 ㎎·g-1 DW, respectively). Moreover, the light treatment group had substantially high contents of total phe- nolic, flavonoid, and rutin compounds (11.273 ± 0.291 gallic acid equivalent (GAE)·㎎·g-1 DW, 9.943 ± 0.28 quercetin equivalent (QE)·㎎·g-1 DW, and 2.136 ± 0.491 ㎎·g-1 DW, respectively). These results revealed that light stimulates the accumulation of secondary metabolites, such as phe- nolics and flavonoids, and enhances antioxidant activity in the adventitious roots of A. lobatum. Conclusions: Light conditions can considerably influence the production of health-beneficial metabolites in the adventitious roots of A. lobatum. This provides an understanding of metabolism in adventitious roots for further experimental studies on environmentally sustainable plant second- ary metabolite production. In this study, we show that suitable root cultures have the potential to be used as supplements in the pharmaceutical and nutraceutical industries.
Abstract
Background: Tissue culture is an important strategy for metabolite accumulation in plants. More- over, adventitious roots can produce high levels of phytochemicals. Actinostemma lobatum is con- sumed as a medicinal plant in South Korea, China, India, and Thailand. In this study, we aimed to identify the effects of darkness and light on metabolic changes and antioxidant activity in the adventitious roots of A. lobatum. Methods and Results: To confirm metabolic changes and antioxidant activity, adventitious roots of A. lobatum were grown under appropriate conditions and separated into dark and light treatment groups. Light conditions were found to more significantly influence the accumulation of natural pigments, such as chlorophyll a [0.136 ± 0.001 ㎎·g-1 dry weight (DW)] and total carotenoids (0.047 ± 0.001 ㎎·g-1 DW), than dark conditions (0.072 ± 0.001 ㎎·g-1 DW and 0.026 ± 0.003 ㎎·g-1 DW, respectively). Moreover, the light treatment group had substantially high contents of total phe- nolic, flavonoid, and rutin compounds (11.273 ± 0.291 gallic acid equivalent (GAE)·㎎·g-1 DW, 9.943 ± 0.28 quercetin equivalent (QE)·㎎·g-1 DW, and 2.136 ± 0.491 ㎎·g-1 DW, respectively). These results revealed that light stimulates the accumulation of secondary metabolites, such as phe- nolics and flavonoids, and enhances antioxidant activity in the adventitious roots of A. lobatum. Conclusions: Light conditions can considerably influence the production of health-beneficial metabolites in the adventitious roots of A. lobatum. This provides an understanding of metabolism in adventitious roots for further experimental studies on environmentally sustainable plant second- ary metabolite production. In this study, we show that suitable root cultures have the potential to be used as supplements in the pharmaceutical and nutraceutical industries.