박 자엽조직을 이용한 효율적인 식물체 분화와 Agrobacterium에 의한 형질전환
Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.)
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초록
This study were carried out for selection ofproper transformation variety and development of efficientregeneration and transformation methods. The number ofshoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dishcontaining on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistantpure lines were wide variation on the deviation. The expressionof GFP was high 67% and 100% at the co-cultivation withAgrobacterium. The effective shoot regeneration planthormone were combination BA and 2,4-D. The number andelongation condition of shoot was good after 4 weeks changewith MS medium supplemented with 1 mg/L BA. Effectivecallus production plant hormone were combination of 3mg/L BA and 0.1 mg/L 2.4-D.
Abstract
This study were carried out for selection ofproper transformation variety and development of efficientregeneration and transformation methods. The number ofshoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dishcontaining on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistantpure lines were wide variation on the deviation. The expressionof GFP was high 67% and 100% at the co-cultivation withAgrobacterium. The effective shoot regeneration planthormone were combination BA and 2,4-D. The number andelongation condition of shoot was good after 4 weeks changewith MS medium supplemented with 1 mg/L BA. Effectivecallus production plant hormone were combination of 3mg/L BA and 0.1 mg/L 2.4-D.
참고문헌 (18)
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