Transcriptome-based identification of water-deficit stress responsive genes in the tea plant, Camellia sinensis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a ‘rain-out shelter’ using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmoduline- like protein (Cam7) and galactinol synthase (Gols4) drought- related genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.
Abstract
A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a ‘rain-out shelter’ using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmoduline- like protein (Cam7) and galactinol synthase (Gols4) drought- related genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.
참고문헌 (43)
- Altschul SF, "Gapped BLAST and PSI-BLAST: A new generation of protein database search program", Nucleic Acids Research, 25, pp.3389~3402 (1997)
- Bartels D, "Drought and salt tolerance in plants", Critical Reviews in Plant Science, 24, pp.23~58 (2005)
- Bezbaruah HP, "Cytological investigation in the family Theacea - I. Chromosome numbers in some Camellia species and allied genera", Carylogia, 24, pp.421~426 (1971)
- Bogeat-Tribo L, "Gradual soil depletion results in reversible changes of gene expression, protein profiles, ecophysiology and growth perfomance in Popolus euphratica, a poplar growing in arid regions", Plant Physiology, 143, pp.876~892 (2007)
- Cabrera C, "Determination of tea components with antioxidant activity", Agricultural and Food Chemistry, 51, pp.4427~4435 (2003)
- Carr MKV, "The climatic requirements of tea. A", Experimental Agriculture, 8, pp.1~14 (1972)
- Chatzidimitriadou K, "Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration", Electronic Journal of Biotechnology, 12, pp.1~9 (2009)
- Chavez MM, "Understanding plant response to drought-from genes to the whole plant", Functional Plant Biology, 30, pp.239~264 (2003)
- Cheruiyot EK, "Polyphenols as potential indicators for drought tolerance in Tea (Camellia sinensis L. O kuntze)", Biosciences Biotechnology Biochemistry, 71, pp.2190~2197 (2007)
- Cheruiyot EK, "Threshold soil water content for growth of tea (Camellia sinensis (L.) O. Kuntze)", Tea, 29, pp.30~38 (2008)
- Conesa Götz S, "Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research", Bioinformatics, 21, pp.3674~3676 (2005)
- Das A, "Identification of differentially expressed gene profiles in young roots of tea (Camellia sinensis (L.) O. Kuntze) subjected to drought stress using suppression subtractive hybridization", Plant Molecular Biology, 30, pp.1088~1101 (2012)
- Frei B, "Antioxidant activity of tea polyphenols in vivo: Evidence from animal studies", Journal of Nutrition, 133, pp.3275S~3284S (2003)
- Gupta S, "Identification of drought tolerant progenies in tea by gene expression analysis", Functional Intergrative Genomics, 12, pp.543~563 (2012)
- Hackett CA, "Construction of a genetic linkage map for Camellia sinensis (tea)", Heredity, 85, pp.346~355 (2000)
- Heath LS, "Studying the functional genomics of the stress responses in Loblolly pine with the expression microarray experiment management system", Comprehensive Functional Genomics, 3, pp.226~243 (2002)
- Kamunya SM, "Quantitative genetic parameters in tea (Camellia sinensis (L.) O. Kuntze): I Combining abilities for yield, drought tolerance and quality traits", African Journal of Science, 3, pp.93~101 (2009)
- Kamunya SM, "Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze)", Tree Genetics and Genomes, 6, pp.915~926 (2010)
- Karori SM, "Antioxidant capacity of different types of tea products", African Journal of Biotechnology, 6, pp.2287~2296 (2007)
- Kondo K, "Chromosome numbers in the genus Camellia", Biotropica, 9, pp.86~94 (1977)
- Lin YS, "Factors affecting the levels of tea polyphenols and caffeine in tea leave", Journal of Agricultural Food Chemistry, 51, pp.1864~1873 (2003)
- Maritim TK, "Physiological and biochemical responses of tea (Camellia sinensis L. O Kuntze)to water-deficit stress", Journal of Horticultural Science &Biotechnology, 90(4), pp.395~400 (2015)
- Mohammad IA, "Molecular analysis of dehydration in plants", Plant Science, 1, pp.21~25 (2010)
- Mphangwe NI, "Screening of tea (Camellia sinensis) for traits associated Molecular markers", Applied Biochemical Biotechnology, 171, pp.437~449 (2013)
- Munnik T, "Osmotic stress activate distinct lipid and MAPK signalling pathway in plants", FEBS Lett, 498 (2001)
- Muoki RC, "A shared response of thaumatin like protein, chitinase and late embryogenesis abundant protein to environmental stresses in tea (Camellia sinensis (L.) O. Kuntze)", Functional Intergrative Genomics, 12(3), pp.565~571 (2012)
- Ngetich WK, "Responses of tea to environment in Kenya. III. Yield and yield distribution", Experimental Agriculture, 37, pp.361~372 (2001)
- Nishizawa A, "Galactinol and raffinose constitute a novel function to protect plants from oxidative damage", Plant Physiology, 147, pp.1251~1263 (2008)
- Rossel JB, "A mutation affecting ascorbate peroxidase 2 gene expression reveals a link between responses to high light and drought tolerance", Plant Cell Environment, 29, pp.269~281 (2006)
- Sambrook J, "Molecular Cloning: A Laboratory Manual" (1989), Cold Spring Harbor Laboratory Press
- Shakeel AA, "Morphological, physiological and biochemical responses of plants to drought stress", African Journal of Agricultural Research, 6, pp.2026~2032 (2011)
- Shalini KV, "Identification of molecular markers associated with mite resistance in Coconut (Cocos nucifera L.)", Genome, 50, pp.35~42 (2007)
- Sharma P, "Differential display-mediated identification of three drought-responsive expressed sequence tags in tea (Camellia sinensis (L.) O. Kuntze)", Journal of Bioscience, 30, pp.231~235 (2005)
- Shinozaki K, "Regulatory network of gene expression in the drought and cold stress responses", Current Opinion in Plant Biology, 6, pp.410~417 (2003)
- Taji T, "Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana", Plant Journal, 29, pp.417~426 (2002)
- Tanaka J, "Estimation of the genome size of tea (Camellia sinensis), Camellia (C. japonica), and their interspecific hybrids by flow cytometry", Journal of the Remote Sensing Society of Japan, 101, pp.1~7 (2006)
- Volk GM, "Galactinol synthase gene expression in melon", American Society of Horticultural Science, 128, pp.8~15 (2003)
- Wachira FN, "Dry matter production and partition in diploid, triploid and tetraploid tea", Journal of Horticultural Science and Biotechnology, 74, pp.507~512 (1999)
- Wachira FN, "Current research on tea in Kenya", Proceedings of 2004 International Conference on O-Cha (Tea) Science, pp.59~65 (2004)
- Wachira FN, "Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers", Genome, 38, pp.201~210 (1995)
- Wisniewki M, "Expressed sequence tag analysis of the response of apple (Malus x domestica Royal Gala) to low temperature and water deficit", Physiology Plant, 133, pp.298~317 (2008)
- Wu H, "De novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea, (Camelia sinensis)", Plant Molecular Biology Report, 31(3), pp.524~538 (2012)
- Xu ZZ, "Effects of soil drought with nocturnal warming on leaf stomatal traits and mesophyll cell ultrastructure of a perennial grass", Crop Science, 49, pp.1843~1851 (2009)