SSR 마커를 이용한 배 유전자원의 유연관계
Genetic relationships of pear germplasms using simple sequence repeat marker
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본 연구는 15개의 SSR 마커를 이용하여 배 유전자원 115 점에 대한 유전적 다양성을 분석하였다. 분석된 마커당대립유전자수는 3 ~ 41개로 평균 16개였으며, 마커의 이용도는 평균 0.966이었다. Hobs는 0.140 ~ 0.929로 평균 0.603 이었으며 Hexp는 0.463~0.904로 평균 0.718로 분석되었다. PIC 값은 0.403 ~ 0.897의 범위에 속하였으며 평균값은 0.692 이었다. 배 유전자원의 유전적 거리에 따라 계통간 유연관계를분석한 결과 지리적 분포와 유전적 특성에 의해 크게 2 개의 그룹으로 구분되었다. 첫 번째 그룹은 유럽종 P. communis 에 속하는 품종으로 구성되었으며 두 번째 그룹은 동양배그룹으로 P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana와 교잡종 및 종의 분류가 명확하지 않은 유전자원이 포함되었다. 본 연구는 배 유전자원의 유전자형 분석을 통하여 유전적 다양성을 가지는 품종들을 분류하였으며, 육종을 위한 기초자료로 활용 가능할 것으로 사료된다.
Abstract
This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity (Hobs) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity (Hexp) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.
참고문헌 (25)
- Barkley NA, "Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs)", Theor Appl Genet, 112, pp.1519~1531 (2006)
- Bell RL, "Genetic resources of temperate fruit and nut Crops I", pp.655~697 (1990), International Society for Horticultural Science
- Bell RL, "Fruit breeding, Vol I : Tree and tropical fruits", pp.441~514 (1996), John Willey & Sons
- Cao Y, "Genetic diversity of cultivated and wild Ussurian Pear (Pyrus ussuriensis Maxim.) in China evaluated with M13-tailed SSR markers", Genet Resour Crop Evol, 59, pp.9~17 (2012)
- and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.), "Theor Appl Genet", 2002, 127~138
- Emanuelli F, "Genetic diversity and population structure assessed by SSR and SNP markers in a large germplasm collection of grape", BMC Plant Biol, 13, pp.39~ (2013)
- Hokanson, S., "Microsatellite (SSR) variation in a collection of Malus (apple) species and hybrids", Euphytica, 118, pp.281~294 (2001)
- Kim DI, "Taxonomy of oriental pear (Pyrus sp.) based on multivariate and RAPD analysis", Ph.D., pp.1998
- 김문섭, "한국에서 수집된 돌배나무의 유전적 다양성", 한국육종학회지, 47(1), pp.45~53 (2015), 한국육종학회[DOI]
- Kimura T, "Identification of Asian pear varieties by SSR analysis", Breed Sci, 52, pp.115~121 (2002)
- Liebhard R, "Development and characterisation of 140 new microsatellites in apple (Malus × domestica Borkh.)", Mol Breed, 10, pp.217~241 (2002)
- Lui K, "PowerMarker: integrated analysis environment for genetic marker data", Bioinformatics, 21, pp.2128~2129 (2005)
- Nishitani C, "Development of novel EST-SSR markers derived from Japanese pear(Pyrus pyrufolia)", Breed Sci, 59, pp.391~400 (2009)
- Phillips RL, "DNA-based markers in plants. Vol. 6" (2001), Springer Science & Business Media
- Powell W, "The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis", Mol Breed, 2, pp.225~238 (1996)
- Sawamura Y, "Identification of Parent-offspring Relationships in 55 Japanese Pear Cultivars Using S-RNase Allele and SSR markers", J Jpn Soc Hortic Sci, 77, pp.364~373 (2008)
- Sehic J, "Genetic diversity in a collection of European pear (Pyrus communis) cultivars determined with SSR markers chosen by ECPGR", Sci Hortic, 145, pp.39~45 (2012)
- Silfverberg-Dilworth E, "Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome", Genomes, 2, pp.202~224 (2006)
- Sneath PHA, "Numerical taxonomy : The principles and practice of numerical classification" (1973), W. H. Freeman
- Teng Y, "Genetic relationships of Pyrus species and cultivars native to East Asia revealed by randomly amplified polymorphic DNA markers", J Am Soc Hortic Sci, 127, pp.262~270 (2002)
- Wünsch A, "Characterization of variability and genetic similarity of European pear using microsatellite loci developed in apple", Sci Hortic, 113, pp.37~43 (2007)
- Weber JL, "Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction", Am J Hum Genet, 44, pp.388~396 (1989)
- Yamamoto T, "SSRs isolated from apple can identify polymorphism and genetic diversity in pear", Thero Appl Genet, 102, pp.865~870 (2001)
- Yamamoto T, "Simple sequence repeats for genetic analysis in pear", Euphytica, 124, pp.129~137 (2002)
- Yamamoto T, "Development of microsatellite markers in the Japanese pear (Pyrus pyrifolia Nakai)", Mol Ecol Not, 2, pp.14~16 (2002)