한국에서 수집된 돌배나무의 유전적 다양성
Genetic Diversity of Wild Pear Accessions Collected in Korea
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초록
이 연구의 목적은 형태적 특성과 microsatellite 마커를 이용하여 국내에서 자생하고 있는 돌배나무 유전자원의 유전적 다양성 평가를 위하여 수행하였다. 돌배나무 14개의 형태적 특성을 조사한 바 수집종간에 높은 변이성을 나타냈으나 환경의 영향을 많이 받는 양적형질이기 때문에 정확한 특성평가가 어려웠다. 50개의 microsatellite 마커를 이용하여 62개 돌배나무 수집개체에 다형성 정도가 높은 16개를 선정하였다. 이들 마커와 돌배나무 유전자원 62점을 검정하였을 때 총284개의 대립유전자가 나타났으며, 마커에 따라 10-27개까지 다양한 분포 양상을 나타냈다. 16개 마커의 평균 PIC 값과 관찰된 이형접합성은 각각 0.836와 0.776로 나타났다. 돌배 62 개 수집개체를 microsatellite 마커에 의해 나타난 대립유전자를 근거로 Jaccard 방법에 따라 산출된 유전적 유사도는 0.09 ~1.00까지 넓은 범위에 속하였고, UPGMA 방법에 따라 군집분석을 실시하였을 때, 2개의 그룹으로 크게 나누어졌으며 55개 돌배나무 수집개체가 구분되는 것으로 나타났다. 본 연구 결과는 돌배나무 수집개체의 유전적 다양성과 유연관계 평가를 통해 유용한 유전자원에 대한 정보를 제공하는데 유용하게 활용될 수 있을 것이다.
Abstract
The objective of this study was carried out to assess genetic diversity of Korean local accessions of wild pear usingmorphological traits and microsatellite markers. Assessment of 14 phenotypic traits showed high variation among wild pearaccessions. These parameters were not applicable genetic diversity analysis of wild pear collection due to quantitatively inheritand their expression is affected by environmental factors. Microsatellite markers were used to evaluate the genetic similarity of62 accessions. Among 50 tested SSR markers, 16 primer pairs were selected to profile genetic diversity on the basis of highlevel polymorphism. These microsatellite markers showed 1 or 2 discrete amplified fragment for all of the accessions. The sixteenmicrosatellite loci amplified 278 alleles, with 10 to 27 alleles (average 17.385) per locus. The mean of observed heterozygosityand polymorphism information content were 0.776 and 0.836, respectively. The Jaccard’s similarity coefficient ranged from 0.09to 1.00. Two major groups were produced from all the accessions by UPGMA cluster analysis. Fifty five accessions could bediscriminated except for 2 pairs. These results would be provided useful information about valuable genetic resources thoughassessment of genetic diversity and relationship in local pear accessions.
참고문헌 (17)
- Bao L, "Genetic diversity and similarity of pear (Pyrus L.)cultivars native to East Asia revealed by SSR (simple sequence repeat) markers", Genet. Resour. Crop Evol, 54, pp.959~971 (2007)
- Bell RL, "Genetic resources of temperate fruit and nut cropsⅠ", pp.665~697 (1990), International Society for Horticultural Science
- 조강희, "AFLP 표지를 이용한 배 유전자원의 유연관계 분석", 한국육종학회지, 41(4), pp.444~450 (2009), 한국육종학회
- Kimura T, "Identification of Asian pear varieties by SSR analysis", Breed. Sci, 52, pp.115~121 (2002)
- Miranda C, "Genetic diversity and structure in a collection of ancient Spanish pear cultivars assessed by microsatellite markers", J. Amer. Soc. Hort. Sci, 135, pp.428~437 (2010)
- Nishitani C, "Development of novel EST-SSR markers derived from Japanese pear (Pyrus pyrifolia)", Breed. Sci, 59, pp.391~400 (2009)
- Liu K, "PowerMarker: an integrated analysis environment for genetic marker analysis", Bioinformatics, 21, pp.2128~2129 (2005)
- Rohlf FJ, "NTSYSpc: Numerical taxonomy and multivariate analysis system, ver. 2.10b" (2000), Applied Biostatistics Inc
- Rugienius R, "Genetic polymorphism of wild pear accessions collected in Lithuania", Baltic Forestry, 19, pp.12~21 (2013)
- Sehic J, "Genetic diversity in a collection of European pear (Pyrus communis) cultivars determined with SSR markers chosen by ECPGR", Sci. Hortic, 145, pp.39~45 (2012)
- Shimura, I, "Heibonsha’s world encyclopedia Vol. 36", pp.354~372 (1988), Heibonsha
- Sneath PHA, "Numerical taxonomy: The principles and practice of numerical classification" (1973), Freeman WH
- Wolko L, "Genetic diversity of European pear cultivars (Pyrus communis L.) and wild pear (Pyrus pyraster (L.) Burgsd.)inferred from microsatellite markers analysis", Genet. Resour. Crop Evol, 57, pp.801~806 (2010)
- Yamamoto Y, "DNA profiling of fresh and processed fruits in pear", Breed. Sci, 56, pp.165~171 (2006)
- Yamamoto T, "Simple sequence repeats for genetic analysis in pear", Euphytica, 124, pp.129~137 (2002)
- Yamamoto T, "Genetic linkage maps constructed by using an interspecific cross between Japanese and European pears", Theor. Appl. Genet, 106, pp.9~18 (2002)
- Liwu Zhu, "Study on Pear (Pyrus spp.) Germplasm Resources Collected at the Protection District of ‘Dangshansuli’ by ISSR Markers", Horticulture, Environment, and Biotechnology, 50(4), pp.334~340 (2009), 한국원예학회