국내에서 수집된 자두의 품종식별을 위한 SSR Profile 데이터베이스 구축
Construction of SSR Profile Database for Variety Identification of Plum Collected in Korea
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국내에서 수집된 자두 38품종의 식별을 위하여 SSR 마커를 이용하여 품종별 SSR 프로파일 데이터베이스를 구축하였다. 자두 품종의 식별에 적합한 마커를 선정하기 위하여 8개품종을 대상으로 총 61개의 마커를 분석하였다. 8개 품종간에 높은 다형성과 재현성을 나타내고, 밴드패턴이 선명한 21 개의 마커를 선발하여 공시된 38품종을 분석하였을 때 총210개의 대립유전자가 분석되었다. 대립유전자의 수의 분포는 3~17개를 나타내었고, 마커당 평균 대립유전자의 수는10개로 분석되었다. PIC 값은 0.549∼0.870의 범위에 속하였으며 평균값은 0.758로 나타났다. 210개의 대립유전자를Jaccard 방법에 의해 유사도를 산출하고 비가중 산술방식에의해 집괴 분석한 결과 공시품종의 유전적 거리는 0.06~1.00의 범위를 나타내었고 21개 SSR 마커에 의해 38품종 중32품종이 식별되었다. 본 결과는 자두 출원품종의 신품종 보호 심사를 위한 유전자 분석 자료, 종자분쟁 발생 시 해결도구로 유용하게 활용될 것으로 사료된다.
Abstract
This study was conducted to construct a DNA marker database for 38 plum varieties collected in Korea using simple sequence repeat (SSR) markers. A set of 61 SSR primer pairs was tested to select polymorphic SSR markers between 8 varieties. Among the 61 primer pairs, 21 showed polymorphism, reproducibility and easy scoring. The genetic relationship between the 21 SSR markers and 38 varieties was analyzed. A total of 210 polymorphic amplified fragments were obtained with the 21 SSR markers. Three to seventeen SSR alleles were detected for each locus, with an average of 10.0 alleles per locus. Average polymorphism information content (PIC) was 0.758, with a range from 0.549 to 0.870. A total of 210 SSR marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis by an unweighted pair-group method with arithmetical average (UPGMA). The genetic distance ranged from 0.06 to 1.00 in 38 varieties. Out of 38 plum varieties, 32 were identified using the 21 SSR markers. Therefore, these SSR markers may be employed to complement distinctness, uniformity, and stability (DUS) tests or as potential tools to solve seed disputes regarding plums.
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