In vitro propagation of Phaleonopsis hybrid ‘Little gem’ by culturing apical part and axillary bud of flower stalk
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The in vitro propagation of the commercially important Phalaeonopsis hybrid ‘Little gem’ was achieved by culturing the apical part and axillary buds excised from flower stalks. The explants were cultured on 5 different basal media: 3.0 g･L-1 Hyponex and 4.0 g･L-1 peptone (H3P4) and Murashige & Skoog (MS) media were shown to be suitable for shoot regeneration. The MS medium supplemented with 5.0 mg･L-1 6-benzylaminopurine (BA) was found to be more efficient for shoot regeneration. However, the number of shoots induced by axillary buds was higher than that induced by the apical part. Incubation of the apical part under darkness for one week, as well as of the explants in the same medium with activated charcoal (AC) 0.5 g･L-1 promoted shoot regeneration and shoot growth; similar growth was not observed with axillary buds.
Abstract
The in vitro propagation of the commercially important Phalaeonopsis hybrid ‘Little gem’ was achieved by culturing the apical part and axillary buds excised from flower stalks. The explants were cultured on 5 different basal media: 3.0 g･L-1 Hyponex and 4.0 g･L-1 peptone (H3P4) and Murashige & Skoog (MS) media were shown to be suitable for shoot regeneration. The MS medium supplemented with 5.0 mg･L-1 6-benzylaminopurine (BA) was found to be more efficient for shoot regeneration. However, the number of shoots induced by axillary buds was higher than that induced by the apical part. Incubation of the apical part under darkness for one week, as well as of the explants in the same medium with activated charcoal (AC) 0.5 g･L-1 promoted shoot regeneration and shoot growth; similar growth was not observed with axillary buds.
참고문헌 (15)
- Arditii J, "Factors affecting the germination of orchid seeds", Bot. Rev, 33, pp.1~97 (1967)
- Chen Y, "Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis", Plant Growth Regulation, 16, pp.99~101 (1995)
- Chen YC, "A reliable protocol for plant regeneration from callus culture of Phalaenopsis", In Vitro Cell Dev Biol-Plant, 36, pp.420~423 (2000)
- Chen JT, "Induction of repetitive embryogenesis from seed-derived protocorms of Phalaenopsis amabilis var. formosa Shimadzu", In vitro Cell Dev Biol-Plant, 40, pp.290~293 (2004)
- Chung JD, "Asymbiotic germination of Cymbidium ensifolium. II. Effects of several supplements to the medium, pH values and light and", or dark culture periods on growth of rhizome and organogenesis for rhizome, 1985, pp.26, 86~92
- Fridborg G, "Effects of activated charcoal on growth and morphogenesis in cell cultures", Physiol. Plant, 34, pp.306~308 (1975)
- Ichihashi S, "Orchid Biology: Reviews and Perspectives, VII", pp.285~316 (1997), Kluwer Academic Publishers
- Kosir P, "Direct shoot regeneration from nodes of Phalaenopsis orchid", Acta Agric Slov, 83, pp.233~242 (2004)
- Aung Htay Naing, "In Vitro Micropropagation and Conservation of Rhynchostylis retusa BL.", Horticulture, Environment, and Biotechnology, 51(5), pp.440~444 (2010), 한국원예학회
- Naing AH, "Efficient plant regeneration of the endangered medicinal orchid, Coelogyne cristata using protocorm-like bodies", Acta Physiol Plant, 33, pp.659~666 (2011)
- Paek KY, "Plant Embryo Culture: Methods and Protocols, In Plant Embryo Culture: Methods and Protocols", Methods Mol Biol, 710, pp.293~306 (2011)
- Park SY, "Rapid propagation of Phalaenopsis from floral stalk-derived leaves", In Vitro Cell Dev Biol-Plant, 3, pp.168~172 (2002)
- Park YS, "Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium", Plant Cell Tiss Organ Cult, 45, pp.79~85 (1996)
- Takahashi K, "Seed germination of Habenaria (pecteilis) radiata (Orchidaceae: Orchideae) in vitro", Lindleyana, 15, pp.59~63 (2000)
- Tokuhara K, "Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds", Plant Cell Rep, 13, pp.7~11 (1993)