In vitro multiple shoot proliferation and plant regeneration in rose (Rosa hybrida L.)
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This study was conducted to investigate an optimal condition for shoot proliferation and regenerate shoots from in vitro leaflet and embryogenic calli from in vitro roots in rose. The effect of BAP on shoot proliferation was somewhat different depending upon genotypes or gelling agents. Leaflets with petiole cut from donor shoots which had been cultured in MS medium supplemented with 0.1 mg·L-1 NAA for six weeks was effective for regeneration of adventitious buds (ABs) as well as shoot elongation of Rosa hybrida cv. Sweet Pink. Culturing seven leaflet explants per petri plate (100 mm × 15 mm) was effective for regeneration of ABs. Embryogenesis was shown in the calli induced from roots of Rosa hybrida cv. Sweet Pink cultured in the SH medium supplemented with 11 mg·L-1 2, 4-D for four weeks. Color of calli induced from roots was yellow although their color was a little different as type of basal medium.
Abstract
This study was conducted to investigate an optimal condition for shoot proliferation and regenerate shoots from in vitro leaflet and embryogenic calli from in vitro roots in rose. The effect of BAP on shoot proliferation was somewhat different depending upon genotypes or gelling agents. Leaflets with petiole cut from donor shoots which had been cultured in MS medium supplemented with 0.1 mg·L-1 NAA for six weeks was effective for regeneration of adventitious buds (ABs) as well as shoot elongation of Rosa hybrida cv. Sweet Pink. Culturing seven leaflet explants per petri plate (100 mm × 15 mm) was effective for regeneration of ABs. Embryogenesis was shown in the calli induced from roots of Rosa hybrida cv. Sweet Pink cultured in the SH medium supplemented with 11 mg·L-1 2, 4-D for four weeks. Color of calli induced from roots was yellow although their color was a little different as type of basal medium.
참고문헌 (16)
- Dohm A., "Somatic embryogenesis in roses", Acta Hort, 547, pp.341~347 (2001)
- Dubois L. A. M., "Preliminary report on the direct regeneration of adventitious buds on leaf explants of in vivo grown glasshouse rose cultivars", Garrenbauwissenschaft, 60, pp.249~253 (1995)
- Famiani F., "Effect of leaf exision time and age, BA concentration and dark treatments on in vitro shoot regeneration of M.26 apple rootstock", J Hort Sci, 69, pp.679~685 (1994)
- Hsia C., "Organogenesis and somatic embryogenesis in callus of Rosa hybrida and Rosa chinensis minima", Plant Cell Tissue and Organ, 44, pp.1~6 (1996)
- Ibrahim R., "Factors controlling high efficiency adventitious bud formation and plant regeneration in vitro leaf explants of roses (Rosa hybrida L.)", Sci Hort, 88, pp.41~57 (2001)
- Kim C. K., "Somatic embryogenesis from in vitro grown leaf explants of Rosa hybrida L", J. Plant Biotechnol, 5, pp.169~172 (2003)
- Kim C. K., "In vitro micropropagation of Rosa hybrida L", J Plant Biotechnol, 5, pp.115~119 (2003)
- Lee Y. K., "Plant regeneration from leaf segment cultures of chrysanthemum (Dendranthema grandiflora Tzvelev)", Kor J Plant Tissue Culture, 26, pp.59~63 (1999)
- Direct regeneration of adventitious buds from in vitro leaf explant of Rosa hybrida ‘Sweet Pink’, "Lee S. Y.", Nat. Hor. Res. Ins. Annual Research Report 2003, 72~73 (2004)
- Lloyd D., "The induction in vitro of adventitious shoots in Rosa", Euphytica, 37, pp.31~36 (1988)
- Marchant R., "Somatic embryogenesis and plant regeneration in floribunda rose (Roas hybrida L.) cvs. Trumpeter and Glad Tidings", Plant Sci, 120, pp.95~105 (1996)
- Murashige T., "A revised medium for rapid growth and bioassay with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Pati P. K., "In vitro propagation of rose-A review", Biotechnol Advances, 24, pp.94~114 (2006)
- Pati P. K., "Direct shoot regeneration from leaf explants of Rosa damascena Mill", In Vitro Cell Dev Biol-Plant, 40, pp.192~195 (2004)
- Schenk R. U., "Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures", Can J Bot, 50, pp.199~204 (1972)
- Van der Salm T. P. M., "Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida L. ‘Moneyway’", Plant Cell Reports, 15, pp.522~526 (1996)