추출 용매에 따른 개똥쑥 차 추출물의 페놀 성분과 항산화 활성
Phenolic content and antioxidant activity of sweet wormwood tea extracts using different solvents
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초록
The selection of a suitable solvent is very important when preparing an extract. However, the effect of ethanol solvent concentration in the extraction of sweet wormwood tea has not been reported. Thus, extracts were prepared from sweet wormwood tea using water and various ethanol concentrations, and the phenolic compounds, anti-oxidants and anti-enzyme activities of the extracts were analyzed. The phenolic acid and flavonoid components differed according to extraction solvent, which also resulted in different antioxidant and antienzyme activities. In particular, flavonoid rhamnetin was not extracted using 80% and 99.5% ethanol and was highest when 60% ethanol was used for extraction. In the case of chlorogenic acid, the highest extraction efficiency was obtained with 80% ethanol. These results suggest the need for research to increase specific extraction efficiency by targeting major compounds that affect physiological activity.
Abstract
The selection of a suitable solvent is very important when preparing an extract. However, the effect of ethanol solvent concentration in the extraction of sweet wormwood tea has not been reported. Thus, extracts were prepared from sweet wormwood tea using water and various ethanol concentrations, and the phenolic compounds, anti-oxidants and anti-enzyme activities of the extracts were analyzed. The phenolic acid and flavonoid components differed according to extraction solvent, which also resulted in different antioxidant and antienzyme activities. In particular, flavonoid rhamnetin was not extracted using 80% and 99.5% ethanol and was highest when 60% ethanol was used for extraction. In the case of chlorogenic acid, the highest extraction efficiency was obtained with 80% ethanol. These results suggest the need for research to increase specific extraction efficiency by targeting major compounds that affect physiological activity.
참고문헌 (34)
- Ames BN, "Oxidants, antioxidants, and the degenerative diseases of aging", PNAS, 90(17), pp.7915~7922 (1993)
- Aranda A, "Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells", Toxicol in Vitro, 27(2), pp.954~963 (2013)
- Aurand LW, "Superoxide and singlet oxygen in milk lipid peroxidation", J Dairy Sci, 60(3), pp.363~369 (1977)
- Bhakuni RS, "Secondary metabolites of Artemisia annua and their biological activity", Curr Sci, 80(1), pp.35~48 (2001)
- Blois MS, "Antioxidant determinations by the use of a stable free radical", Nature, 181(4617), pp.1199~ (1958)
- Charles DJ, "Germplasm variation in artemisinin content of Artemism annua using an alternative method of artemisinin analysis from crude plant extracts", J Nat Prod, 53(1), pp.157~160 (1990)
- Gramza A, "Antioxidant activity of tea extracts in lipids and correlation with polyphenol content", Eur J Lipid Sci Tech, 108(4), pp.35~ (2006)
- Huie RE, "The reaction of NO with superoxide", Free Radical Res, 18(4), pp.195~199 (1993)
- 김선아, "에탄올 농도에 따른 머루(Wild Grape, Vitis coignetiae) 추출물의 폴리페놀 함량 및 항산화 활성", 한국식품영양과학회지, 36(12), pp.1491~1496 (2007), 한국식품영양과학회
- 강경진, "HaCaT 각질형성세포에서 개똥쑥(Artemisia annua L) 유래 성분인 Artemisinic acid의 Macrophage-derived Chemokine 억제 효과", 생약학회지, 43(3), pp.217~223 (2012), 한국생약학회
- 김보민, "HepG2 간암세포에서 미토콘드리아 경로를 통한 개똥쑥 추출물의 Apoptosis 유도 효과", 한국식품영양과학회지, 45(12), pp.1708~1716 (2016), 한국식품영양과학회
- 김경철, "에탄올 농도에 따른 돌외 잎 차 추출물의 생리활성", Journal of Plant Biotechnology, 46(1), pp.37~44 (2019), 한국식물생명공학회[DOI]
- 고현민, "발효 산삼배양근 초임계 유체 추출물의 생리활성 검정", 공학기술논문지, 11(2), pp.115~121 (2018), 공학기술연구원[DOI]
- Konczak I, "Antioxidant capacity and phenolic compounds in commercially grown native Australian herbs and spices", Food Chem, 122(1), pp.260~266 (2010)
- Lee JC, "Extract from Rhus verniciflua Stokes is capable of inhibiting the growth of human lymphoma cells", Food Chem Toxicol, 42(9), pp.1383~1388 (2004)
- 이상수, "개똥쑥 추출물을 첨가한 김치의 품질특성", Food Science and Preservation, 22(5), pp.666~673 (2015), 한국식품저장유통학회
- Li J, "Scavenging effects of phenylpropanoid glycosides on superoxide and its antioxidation effect", Zhongguo Yao Li Xue Bao, 13(5), pp.427~430 (1992)
- Li Y, "Medicinal chemistry of bioactive natural products", pp.183~256 (2006), Wiley-Interscience
- Milella L, "α -Glucosidase and α-amylase inhibitors from Arcytophyllum thymifolium", J Nat Prod, 79(8), pp.2104~2112 (2016)
- 문은우, "개똥쑥 분말을 첨가한 전병의 특성", 한국식생활문화학회지, 30(5), pp.644~649 (2015), 한국식생활문화학회[DOI]
- 문은우, "개똥쑥 분말을 첨가한 절편의 품질특성", Food Science and Preservation, 22(6), pp.811~816 (2015), 한국식품저장유통학회
- Patel R, "In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods", JAPER, 1, pp.52~68 (2011)
- Pereira DF, "Effects of flavonoids on α-glucosidase activity: potential targets for glucose homeostasis", Nutrition, 27(11-12), pp.1161~1167 (2011)
- Prior RL, "Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC (FL))) of plasma and other biological and food samples", J Agric Food Chem, 51(11), pp.3273~3279 (2003)
- Proteggente AR, "The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition", Free Radic Res, 36(2), pp.217~233 (2002)
- Robak J, "Flavonoids are scavengers of superoxide anions", Biocheem Pharmacol, 37(5), pp.837~841 (1988)
- 류지현, "개똥쑥의 항산화 및 항암활성과 기능성 물질의 탐색", 한국식품영양과학회지, 40(4), pp.509~516 (2011), 한국식품영양과학회
- Tan RX, "Biologically active substances from the genus Artemisia", Planta medica, 64(04), pp.295~302 (1998)
- Tsao R, "Chemistry and biochemistry of dietary polyphenols", Nutrients, 2(12), pp.1231~1246 (2010)
- Tylor BS, "Nitric oxide down regulates hepatocyte-inducible nitric oxide synthase gene expression", Arch Sur, 132(11), pp.1177~1183 (1997)
- van der Kooy F, "The content of artemisinin in the Artemisia annua tea infusion", Planta medica, 77(15), pp.1754~1756 (2011)
- Weathers PJ, "The flavonoids casticin and artemetin are poorly extracted and are unstable in an Artemisia annua tea infusion", Planta medica, 78(10), pp.1024~1026 (2012)
- Willcox M, "Traditional medicinal plants and malaria", pp.43~59 (2004), CRC press
- Xing N, "Quercetin inhibits the expression and function of the androgen receptor in LNCaP prostate cancer cells", Carcinogenesis, 22(3), pp.409~414 (2001)