지황 부정근을 이용한 식물체 재분화 및 생물반응기 배양
Plant Regeneration from Adventitious Roots of Rehmannia glutinosa Liboschitz and Bioreactor Culture
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지황의 부정근을 이용한 기내묘 생산 체계를 확립하였다. 먼저 지황의 잎절편을 2 mg/L IBA 혹은 2 mg/L NAA를 첨가 한 MS 고체배지에 치상하여 부정근을 유도하였다. 고체배지 에서의 효율적인 증식조건을 확인하기 위해 다양한 옥신 농 도에 따른 배지에 부정근을 치상하여 4주간 배양한 후 증식 된 부정근의 수와 길이를 측정한 결과 2 mg/L IBA 조건에서 최적의 생장을 보였다. 상기실험에서 확립된 부정근 증식조건 을 이용하여 액체배양을 실시하였으며, 이를 액체 현탁배양하 여 액체조건에서의 증식조건을 확립하였다. 또한, 2 mg/L IBA 가 첨가된 1/3 MS배지 조건에서 생물반응기에 10 g의 부정근 을 접종하여 6주간 암배양하여 255 g의 부정근을 생산할 수 있었으며, 이렇게 생산된 부정근은 2 mg/L BA가 첨가된 MS 고체배지에서 신초를 유도하였다. 신초들은 모두 정상적인 생 육을 보이며, 식물체로 생장하였으며, 이들 식물체들은 토양으 로 옮겨 순화되었다. 따라서 이러한 증식 체계를 이용하여 지 황식물체의 대량증식체계를 확립하였다.
Abstract
This experiment was carried out to develop rapid mass propagation via shoot organogenesis systemfrom adventitious roots of Rehmannia glutinosa . The induction of adventitious roots from leaf explants was mostfavorable to MS solid medium supplemented with 2 mg/L IBA. However, the growth of adventitious roots washighest when they were cultured on 1/3 strength MS liquid medium supplemented with 2 mg/L IBA. When theadventitious roots were grown in 10 L bioreactor, 10 g roots as initial inoculum was increased to 225 g after 6 weeksof culture. The harvested roots were cultured onto solid medium to induce plant regeneration. The optimaladventitious shoot formation was observed on MS medium supplemented with 2 mg/L BA. Rooting of individualshoots was induced after transfer to half strength MS medium without growth regulators. Plantlets afteracclimatization were successfully transplanted in the field and no phenotypic variation was observed among them.
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