홍경천 (Rhodiola sachalinensis A. Bor) 잎절편으로부터 식물체 재분화에 미치는 Cytokinin과 Putresine의 영향
Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor
0
조회
0
다운로드
3
인용
0
참고문헌
초록
홍경천 (Rhodilola sachalinensis A. Bor)의 잎절편로부터 부정아를 유도하고자 BA와 kinetin을 처리한 결과, BA의 처리가 kinetin의 처리보다 부정아 유도와 신초의 생장에 양호한 것으로 나타났으며, 특히 BA 1 mg/L처리에서 부정 아 유도빈도와 신초길이는 각각 71%와 3.0 mm로 가장 높게 나타났다. 또한 고빈도의 부정아를 유도하고자 다양한 농도의 putrescine를 처리한 결과, putrescine 100 mM 처리에서 부정아 유도빈도가 BA 1 mg/L 단용처리보다 약 20%가 증가되었고 신초길이 역시 약 10 mm가 증가된 것으로나타났다. 이상의 결과에서 유도된 부정아는 생장조절물질이 포함되지 않은 1/2MS배지에서 발근과 신장이 정상적으로 이루어졌으며 약 7 cm 정도 생장한 유식물체는 peatmoss: and가 1:1로 혼합된 상토에서 약 95%이상 정상적으로 순화되었다.
Abstract
The effects of cytokinin and putrescine on adventitious shoot induction from leaf explant ofRhodiola sachalinensis A. Bor were investigated. Among cytokinin used in this experiment, BA was more efective on adventitious shoot induction and shoot elongation than kinetin. Especially, 1 mg/L BA was the best to increase adventitious shoot induction (71%) and shoot elongation (3.0 mm). In addition, 100 mM putrescine in MS medium with 1 mg/L BA was higher in adventitious shoot induction (93%) and shoot elongation (3.8 mm) than single treatment of 1 mg/L BA. Adventitious shoots induced in this experiment rooted on 1/2 MS medium and acclimated over 95% on comp osed soil (peatmoss:sand=1:1).
참고문헌 (22)
- Ahn JH, "Effects of growth regulators on calus induction and plant regeneration from leaf explants of Sedium sarmentation", 31, pp.25~29 (2004)
- Crisosto CH, "Putrescine extends effective polination period in Comice pear irrespective of post-anthesis ethylene levels", pp.211~221 (1992)
- Crisosto CH, "and fruit set in Comeice pear", pp.1 708~712
- Han X, "Separation of salidroside from Rhodiola crenukata by high speed counter current chromatography", 971, pp.237~241 (2002)
- Jiang M, "Studies on producing effective medicinal ingredients of Rhodiola sachalinensis by tissue culture", pp.355~359, Chin J Shen Univ 25
- Kim SJ, "Production of salidroside from callus culture of Rhodiola sachalinensis A", 31, pp.89~94 (2004)
- Lee MW, "Antioxidant phenolic compounds from the roots of Rhodiola sachalinensis A", 23, pp.455~458 (2000)
- Li HB, "Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiolasachalinensis by high-speed counter-current chromatography", 932, pp.91~95 (2001)
- Kim YH, "Quantitative determination of salidroside and tyrosol fromthe chromatography", 23, pp.349~352 (2000), Linch PT
- Skoog F, "A revised medium for rapidgrowth and bioassays with tobacco tissue cultures", pp.473~479 (1962)
- Mosharoff A, "Effects of alcohol aqueousextract from Rhodiola rose L root on learning andmemory", pp.3~16 (1986)
- Pradhan C, "through in vitro shoot proliferationfrom cotyledonary nodes", 18, pp.122~126 (1998)
- Dave A, "Micropropagation of Sterculaurens Roxb", 15, pp.704~706 (1996)
- Singhvi A, "Micropropagation of Achrasapota through enhanced axillary ranching", 76, pp.219~229 (1998)
- Soumendra NK,, "Highfrequency axillary shoot proliferation and plant regenerationfrom cotyledonary nodes of pomegranate", 85, pp.261~270 (2000)
- Valero D, "Polyamine roles on the post-harvest of fruits", pp.39~55 (1999)
- Wu SX, "High yield production ofsalidroside in suspension culture of Rhodiola sachalinensis", 106J Biotech, pp.33~43 (2003)
- Xu JF, "Activity of tyrosol glucosyltransferaseand improved salidroside production throughbiotransformation of tyrosol in Rhodiola sachalinensiscell culture", 61, pp.69~73 (1998)
- Xu JF, "Suspension culture ofcompact callus aggregate of Rhodiola sachalinensis forimproved salidroside production", 23, pp.20~27 (1998)
- Yan XF, "Soilnutrient factors related to salidroside production ofRhodiola sachalinensis distributed in Chang Bai Mountain", 53, pp.267~276 (2004)
- Yoon ES, "Effect of plant growth regulators on plantregeneration from leaf and stem explant culture ofSedium erythrostichum Miq", 24, pp.285~289 (1997)
- Zhong Y, "Phenolic constituents of Rhodiola coccinea atibetan folk medicine" (1991)