Shoot regeneration from internode sections of Ardisia pusilla DC.
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초록
This study was conducted to regenerate shoots from internode sections (about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body (PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 mg･L-1 thidiazuron (TDZ) and 0.5 mg･L-1 indole-3-butyric acid (IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.
Abstract
This study was conducted to regenerate shoots from internode sections (about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body (PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 mg･L-1 thidiazuron (TDZ) and 0.5 mg･L-1 indole-3-butyric acid (IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.
참고문헌 (16)
- Achkor H., "Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis", Plant Physiol, 132, pp.2248~2255 (2003)
- Mold. (Verbenaceae), "In Vitro Cell Dev Biol_Plant", 2006, 235~239
- Cuenca B., "In vitro adventitious bud regeneration from internode segments of beech", Plant Cell Tissue and Culture, 62, pp.213~220 (2000)
- Goo D. H., "Micropropagation of Ardisia pusilla and Ardisia Japonica in vitro", Acta Hort, 766, pp.237~241 (2008)
- Kang G. H., "In vitro mass propagation of Ardisia pusilla DC", J Plant Biotechnol, 32, pp.281~285 (2005)
- Kelkar S. M., "Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link", Plant Cell Reports, 17, pp.721~725 (1998)
- Kim, K. J., "Air filtration using Korean native plant", The tenth anniversary symposium of foundation of Korean wild plant research, pp.15~31 (2007)
- Kulkarni, A. A., "Direct shoot regeneration from node, internode, hypocotyls and embryo explants of Withania somnifera", Plant Cell Tissue and Culture, 62, pp.203~209 (2000)
- Lee C. H., "The use of 6-benzylaminopurine for lateral branching of Ardisia pusilla", J Kor Soc Hort Sci, 46, pp.396~401 (2006)
- Lin C. C., "In-vitro culture of flower stalk internodes of phalaenopsis and doritaenopsis", Lindleyana, 1, pp.158~163 (1986)
- Lu C. Y., "The use of thidiazuron in tissue culture", In vitro Cell Dev Biol, 29, pp.92~96 (1993)
- Luft J. H., "Compounding of Luft’s epon embedding medium for use in electron microscopy with reference to anhydride", Epoxide ratio adjustment Mikroskopie, 29, pp.337~342 (1973)
- Matt A., "In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.)", Plant Cell Rep, 24, pp.468~476 (2005)
- Ministy of Agr. & For, , Flower production in Korea, ~ (2006)
- Poovaiah C. R., "In vitro adventitious shoot regeneration of native spearmint using intermodal explants", Hortscience, 41, pp.414~417 (2006)
- Sanikhani M., "TDZ induces shoot regeneration in various Kalanchoë blossfeldiana Poelln. cultivars in the absence of auxin", Plant Cell Tissue and Organ Culture, 85, pp.75~82 (2006)