Expression of Arabidiopsis CAX4 in tomato fruits increases calcium level with no accumulation of other metallic cations
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초록
We generated transgenic tomato plants with Arabidopsis thaliana H+/cation exchanger gene (CAX4) by Agrobactrium-mediated transformation. We confirmed transgene copy number and transcription by Southern and Northern blot analyses. The intact CAX4-expressing tomato (Lycopersicon esculentum) fruits contained 63-71% more calcium (Ca2+) than wild-type fruits. Moreover, ectopic expression of CAX4 in tomato fruits did not show any significant increase of the four kinds of metallic cations analyzed (Mg2+, Fe2+, Mn2+, and Cu2+). The CAX4-expressing tomato plants including their fruits did not show any morphological alternations during whole growth period. These results suggest the enhanced Ca-substrate specificity of CAX4 exchanger in tomato. Therefore, intact CAX4 exchanger can be a useful tool for Ca2+ nutrient enrichment of tomato fruits with reduced accumulation of undesirable cations.
Abstract
We generated transgenic tomato plants with Arabidopsis thaliana H+/cation exchanger gene (CAX4) by Agrobactrium-mediated transformation. We confirmed transgene copy number and transcription by Southern and Northern blot analyses. The intact CAX4-expressing tomato (Lycopersicon esculentum) fruits contained 63-71% more calcium (Ca2+) than wild-type fruits. Moreover, ectopic expression of CAX4 in tomato fruits did not show any significant increase of the four kinds of metallic cations analyzed (Mg2+, Fe2+, Mn2+, and Cu2+). The CAX4-expressing tomato plants including their fruits did not show any morphological alternations during whole growth period. These results suggest the enhanced Ca-substrate specificity of CAX4 exchanger in tomato. Therefore, intact CAX4 exchanger can be a useful tool for Ca2+ nutrient enrichment of tomato fruits with reduced accumulation of undesirable cations.
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