Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.
Abstract
Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.
참고문헌 (35)
- Ayabe M, "Establishment of a novel tissue culture method, stem-disc culture and its practical application to micropropagation of garlic (Allium sativum L.)", Plant Cell Rep, 17, pp.773~779 (1998)
- Ayabe M, "Regeneration of whole plants from protoplasts isolated from tissue cultured shoot primordia of garlic (Allium sativum L.)", Plant Cell Rep, 15, pp.17~21 (1995)
- Barandiaran X, "An efficient method for callus culture and shoot regeneration of garlic (Allium sativum L)", Hort Science, 34, pp.348~349 (1999)
- Baruch ER, "Virus-free plants of Iris “Wedgewood” obtained by meristem culture", Neth. J. Plant Pathol, 72, pp.270~273 (1966)
- Boltenkov EV, "Cultivation of Iris ensata Thunb. callus tissue, Prikl", Biokhim Mikrobiol, 40, pp.244~251 (2004)
- Gozu Y, "In vitro propagation of Iris pallida Plant Cell Rep", Nakamura M(1993)In vitro propagation of Iris pallida Plant Cell Rep, 13, pp.12~16 (1993)
- Guo DP, "Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai)", Plant Cell Tissue Organ Cult, 83, pp.123~127 (2005)
- Hussey G, "Totipotency in tissue explants and callus of some members of the Liliaceae, Iridaceae and Amaryllidaceae", J Exp Bot, 26, pp.253~262 (1975)
- Ichihashi S, "Clonal propagation of Iris kaempferi by means of flower organ culture", Bull Aichi Univ Edu, 35, pp.135~143 (1986)
- Jehan H, "Plant regeneration of Iris pallida Lam. and Iris germanica L. via somatic embryogenesis from leaves, apecs and young flowers", Plant Cell Rep, 13, pp.671~675 (1994)
- Kawase K, "Propagation of Iris by tissue culture. 2. Culture of flower organs", J Jpn Soc Hort Sci, 60, pp.436~437 (1991)
- Kawase K, "Shoot formation on floral organs of Japanese Irisin vitro", J Jpn Soc Hort Sci, 64, pp.143~148 (1995)
- Lee YN, "New taxa on Korean flora (1)", Korean Jour Botany, 17(1), pp.33~35 (1974)
- 이진실, "한반도 야생란의 분포 및 보호 대상 식물", 식물분류학회지, 36(4), pp.335~360 (2006), 한국식물분류학회
- Report on study of a new species of Iridaceae plants Seoul Agricultural College, "Lee WJ", 1~4 (1964)
- Laublin G, "In vitro ovary culture of some apogon garden Irises (Iris pseudacorus L, I. setosa Pall, I. versicolor L.)", Bot Acta, 105, pp.319~322 (1992)
- Lloyd E, "Commercially feasible micropropagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture", Comb Proc Int Plant Prop Soc, 30, pp.421~427 (1980)
- Luciani GF, "Effects of explants and growth regulators in garlic callus formation and plant regeneration", Plant Cell Tissue Organ Cult, 87, pp.139~143 (2006)
- Mielke KA, "In vitro bulblet formation in Dutch Iris", Hortscience, 24, pp.1028~1031 (1989)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Myers JM, "Regeneration of garlic callus as affected by clonal variation, plant growth regulators and culture conditions over time", Plant Cell Rep, 19, pp.32~36 (1999)
- Radojevic L, "Plant regeneration of Iris setosa Pall. through somatic Embryogenesis and organogenesis", J Plant Physiol, 139, pp.690~696 (1992)
- Radojevic L, "Somatic embryogenesis in tissue culture of Iris (Iris pumila L.)", Acta Hort, 212, pp.719~723 (1987)
- SAS, "SAS", STAT User’s guide (8.02), 2001
- Schulze W, "Wild-Iris für den Garten Fortschritt Erfurt Jena", ~ (1988)
- Shibata K, "A cyclopedia of useful plants and plant products", pp.514~519 (1998), Hokuryukan Tokyo
- Shibli RA, "Somatic embryogenesis in the endemic black Iris", Plant Cell Tiss Org Cult, 61, pp.15~21 (2000)
- Shimizu K, "Plant regeneration from suspension culture of Iris germanica", Plant Cell Tissue and Organ Culture, 50, pp.27~31 (1997)
- Shimizu K, "Plant regeneration from protoplasts of Iris germanica L", Euphytica, 89, pp.223~227 (1996)
- Wang WC, "A novel approach for efficient plant regeneration from long-term suspension culture of Wheat", Plant Cell Rep, 8, pp.639~642 (1990)
- Wang Y, "Efficient plant regeneration from suspension-cultured cells of tall bearded Iris", Hortscience, 34, pp.730~735 (1999)
- Wang Y, "Improved plant regeneration from suspension-cultured cells of Iris germanica L. ‘Skating Party’", Hortscience, 34, pp.1271~1276 (1999)
- Xu Z, "Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation", Acta Physiol Plant, 40, pp.521~528 (2008)
- Yabuya T, "In vitro propagation of Japanese garden Iris, Iris ensata Thunb", Euphytica, 57, pp.77~81 (1991)
- Zheng S, "Factors influencing induction, propagation and regeneration of mature zygotic embryo-derived callus from Allium cepa", Plant Cell Tissue Organ Cult, 53, pp.99~105 (1998)