무측지성 국화 형질전환 계통 영양번식 제2세대의 형태적 및 분자생물학적 특성
Phenotypic and molecular characteristics of second clone (T0V2) plants of the LeLs-antisense gene-transgenic chrysanthemum line exhibiting non-branching
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This study examined the phenotypic and molecular characteristics of the 2nd clone (T0V2) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the nptⅡ gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.
Abstract
This study examined the phenotypic and molecular characteristics of the 2nd clone (T0V2) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the nptⅡ gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.
참고문헌 (15)
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