저온저항성 유전자가 도입된 국화 형질전환체 특성
The characterization of transgenic Chrysanthemum under low temperature condition
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and Ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at 5℃ were much similar to of wild-type plant cultured on 25℃. It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.
Abstract
Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and Ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at 5℃ were much similar to of wild-type plant cultured on 25℃. It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.
참고문헌 (21)
- Anchordoguy T. J., "Modes of interaction of cryoprotectants with membrane phospholipids during freezing", Cryobiology, 24, pp.324~331 (1987)
- Choi K. H., "Expression of cold-regulated gene in transgenic Solanum tuberosum L", Korean J Plant Tissue Culture, 23, pp.311~315 (1996)
- Fu P., "Sucrose content and freezing tolerance of Brassica napus canola (rapeseed) seedlings over expressing an Escherichia coli inorganic pyrophosphatase", 10th international rapeseed congress (1999)
- Han S. G., "Genetic transformation of chrysanthemum with cold regulated gene (BN115)", Korean J Plant Biotechnol, 33, pp.19~25 (2006)
- Holmström K. O., "Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine", Journal of Experimental Botany, 51, pp.177~185 (2000)
- Hwang C. H., "Cold-induced genes of plants with emphasis on their function", Molecular Biology News, 6, pp.57~59 (1994)
- Jeong J. H., "Transformation of Lettuce using cold regulated gene (BN115)", Korean J Plant Tissue Culture, 27, pp.7~12 (2000)
- Lee B. J., "in SPAD chlorophyll value of chrysanthemum (Dendranthema grandiflora Tzvelev) by photoperiod and light intensity", Kor J Hort Sci ＆ Technol, 19, pp.555~559 (2001)
- Levitt, J., "Responses of Plants to Environmental Stresses" (1980), Academic Press
- Livingston D. P. III, "Apoplastic sugars, fructans, fructan exohydrolase, and invertase in winter oat: responses to second-phase cold hardening", Plant Physiol, 116, pp.403~408 (1998)
- Olien C. R., "Ice adhesions in relation to freeze stress", Plant Physiol, 60, pp.499~503 (1977)
- Robinson K. E. P., "Transformation of floriculture crops", Sci. Hort, 55, pp.83~99 (1993)
- Rudolph A. S., "Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline", Cryobiology, 22, pp.367~377 (1985)
- Steponkus P. L., "Role of the plasma membrane in freezing injury and cold acclimation", Annu Rev Plant Physiol, 35, pp.543~584 (1984)
- Steponkus P. L., "A contrast of the cryostability of the plasma membrane of winter rye and spring oat-two species that widely differ in their freezing tolerance and plasma membrane lipid composition", Advances in Low-Temperature Biolog, 2, pp.211~312 (1993)
- Steponkus P. L., "Membrane destabilization during freeze-induced dehydration", Curr Top Plant Physiol, 10, pp.37~47 (1993)
- Strauss G., "Stabilization of lipid bilayer vesicles by sucrose during freezing", Proc Natl Acad Sci USA, 83, pp.2422~2426 (1986)
- Weretilnyk E., "Characterization of three related low-temperature-regulated cDNAs from winter Brassica napus", Plant Physiol, 101, pp.171~177 (1993)
- White T. C., "Regulation of BN115, a low-temperature-responsive gene from winter Brassica napus", Plant physiol, 106, pp.917~928 (1994), 917-928
- Yoon Y. H., "Changes in SPAD value and phothosynthetic rate during grain filling of Oryza glaberrima strains and Oryza sativa cultivars", Korean Journal of Crop Science, 42, pp.759~765 (1997)
- Yun, D. J., "Molecular mechanism of plant adaption to high salinity", Korean J Plant Biotechnol, 32, pp.1~14 (2005)