벼물바구미 (Lissorhoptrus oryzophilus) 내충성 GM 벼에서 T-DNA와 게놈의 인접부위 분석
Analysis of junction site between T-DNA and plant genome in Lissorhoptrus oryzophilus resistance GM rice
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초록
Four transgenic rice lines harboring insect-resistantgene cry3A showed ideal field performances characterizedby high considerable resistance to rice water weevil (Lissorhoptrusoryzophilus Kuschel). In this study, we estimatedthe insertion number of foreign genes, and analyzed theflanking sequences of T-DNAs in rice genome. As a result,T-DNA of BT12R1 line was inserted in exon region of ricechromosome 10. Two copies of T-DNAs were inserted inline BT12R2. BT12R3 line was analyzed at only left borderflanking sequence. BT12R4 line was confirmed one copy offoreign gene insertion at the position 24,516,607 ~ 24,516,636of rice chromosome 5, accompanied by a deletion of 30 bpknown genomic sequences. This intergenic position wasconfirmed none of expressed gene and any deletion/additionof T-DNA sequence. In conclusion, these molecular data ofrice water weevil resistant Bt rice would be used to conductthe biosafety and environment risk assessment for GM cropcommercialization.
Abstract
Four transgenic rice lines harboring insect-resistantgene cry3A showed ideal field performances characterizedby high considerable resistance to rice water weevil (Lissorhoptrusoryzophilus Kuschel). In this study, we estimatedthe insertion number of foreign genes, and analyzed theflanking sequences of T-DNAs in rice genome. As a result,T-DNA of BT12R1 line was inserted in exon region of ricechromosome 10. Two copies of T-DNAs were inserted inline BT12R2. BT12R3 line was analyzed at only left borderflanking sequence. BT12R4 line was confirmed one copy offoreign gene insertion at the position 24,516,607 ~ 24,516,636of rice chromosome 5, accompanied by a deletion of 30 bpknown genomic sequences. This intergenic position wasconfirmed none of expressed gene and any deletion/additionof T-DNA sequence. In conclusion, these molecular data ofrice water weevil resistant Bt rice would be used to conductthe biosafety and environment risk assessment for GM cropcommercialization.
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