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국화 유전체 연구의 동향

Current status and prospects of chrysanthemum genomics

김정선|강상호|손성한|원소윤
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2016.09|Journal of Plant Biotechnology|제43권 제3호|pp. 272-280

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Next-generation sequencingTranscriptomeMolecular markersCompositaeMolecular breeding
주제분류농학

초록

국화는 관상용, 약용으로 활용되는 주요한 화훼 작물중의하나이다. 국화의 육종 프로그램은 다양한 품종의 개발에기여하였으나, 다른 주요한 식량, 채소작물에서 보여졌듯이 전통적인 표현형 기반의 품종선발에서 분자표지를 활용한 선발로 진일보할 필요가 있다. 이러한 분자육종은 유전학, 분자생물학, 최근에는 유전체 연구로 규명된 형질연관분자표지에 의존한다. 그러나 자가불화합성, 자식약세, 이질육배체, 이형접합성, 거대한 유전체와 같은 국화의 생식적, 유전적, 유전체의 특성으로 인하여 이러한 연구는 심각하게 지연되고 있다. 그럼에도 불구하고 유전연구를 통하여 국화의 유전자지도가 구축되었고 꽃, 잎, 식물구조와 같은 국화의 주요한 형질과 연관된 분자표지가 규명되었다. 염기서열 분석기술이 발달됨에 따라 국화의 전사체가 해독되어 국화의 표준유전자 목록이 구축되고 발달단계에 따라혹은 생물적･비생물적 환경에서 특이적으로 발현되는 유전자도 규명되었다. 또한 2배체인 야생의 국화속 식물의 유전체 해독 프로젝트가 시작되었다. 이러한 대량의 염기서열 정보는 국화의 분자육종을 위한 근원적인 자원으로 활용될 수 있을 것이다. 이 총설에서는 국화의 분자유전학, 유전체 연구의 현황을 요약하고 향후 전망을 논의한다.

Abstract

Chrysanthemum is one of the top floriculture species with ornamental and medicinal value. Although chrysanthemum breeding program has contributed to the development of various cultivars so far, it needs to be advanced from the traditional phenotype-based selection to marker-assisted selection (molecular breeding) as shown in major cereal and vegetable crops. Molecular breeding relies on trait-linked molecular markers identified from genetic, molecular, and genomic studies. However, these studies in chrysanthemum are significantly hampered by the reproductive, genetic, and genomic properties of chrysanthemum such as self-incompatibility, inbreeding depression, allohexaploid, heterozygosity, and gigantic genome size. Nevertheless, several genetic studies have constructed genetic linkage maps and identified molecular markers linked to important traits of flower, leaf, and plant architecture. With progress in sequencing technology, chrysanthemum transcriptome has been sequenced to construct reference gene set and identify genes responsible for developments or induced by biotic or abiotic stresses. Recently, a genome sequencing project has been launched on a diploid wild Chrysanthemum species. The massive sequencing information would serve as fundamental resources for molecular breeding of chrysanthemum. In this review, we summarized the current status of molecular genetics and genomics in chrysanthemum and briefly discussed future prospects.

참고문헌 (47)

  1. Abdurakhmonov IY, "Application of association mapping to understanding the genetic diversity of plant germplasm resources", International Journal of Plant Genomics, 2008, pp.574927~ (2008)
  2. Anderson NO, "Flower breeding and genetics: issues, challenges and opportunities for the 21st century", pp.389~437 (2006), Springer Science & Business Media
  3. Barker MS, "Multiple paleopolyploidizations during the evolution of the Compositae reveal parallel patterns of duplicate gene retention after millions of years", Molecular Biology and Evolution, 25, pp.2445~2455 (2008)
  4. cultivars revealed by ISSR analysis, "Acta Horticulturae Sinica", 2007, 1243~1248
  5. Chen S, "Analysis of expressed sequence tags (ESTs) collected from the inflorescence of chrysanthemum", Plant Molecular Biology Reporter, 27, pp.503~510 (2009)
  6. Choi H, "Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X", Plant Molecular Biology, 88, pp.233~248 (2015)
  7. Grattapaglia D, "Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudotestcross:mapping strategy and RAPD markers", Genetics, 137, pp.1121~1137 (1994)
  8. Hodgins KA, "Genomics of Compositae crops: reference transcriptome assemblies and evidence of hybridization with wild relatives", Molecular Ecology Resources, 14, pp.166~177 (2014)
  9. Hong Y, "Transcriptomic analyses reveal species-specific light-induced anthocyanin biosynthesis in chrysanthemum", BMC Genomics, 16, pp.202~ (2015)
  10. Huang H, "cDNA-AFLP analysis of salt-inducible genes expression in Chrysanthemum lavandulifolium under salt treatment", Journal of Plant Physiology, 169, pp.410~420 (2012)
  11. Huang X, "Natural variations and genome-wide association studies in crop plants", Annual Review of Plant Biology, 65, pp.531~551 (2014)
  12. Jo Y, "Transcriptomic landscape of chrysanthemums infected by Chrysanthemum stunt viroid", Plant Omics Journal, 7, pp.1~ (2014)
  13. Kim JS, "Karyotypes of metaphase chromosomes in diploid populations of Dendranthema zawadskii and related species (Asteraceae) from Korea: diversity and evolutionary implications", Journal of Plant Research, 116, pp.47~54 (2003)
  14. 김정성, "국화속 구절초무리와 근연종인 산국 및 감국 에서 보이는 잎의 해부학적 특징", 식물분류학회지, 39(1), pp.29~34 (2009), 한국식물분류학회
  15. Kim SJ, "Phylogenetic analysis of Korean native Chrysanthemum species based on morphological characteristics", Scientia Horticulturae, 175, pp.278~289 (2014)
  16. Kirst M, "Genetic Engineering: Principles and Methods, Volume 26", pp.105~142 (2012), Springer
  17. Klie M, "Strigolactone pathway genes and plant architecture: association analysis and QTL detection for horticultural traits in chrysanthemum", Molecular Genetics and Genomics, 291, pp.957~969 (2016)
  18. Kumar A, "Secondary metabolites of Chrysanthemum genus and their biological activities", Current Science, 89, pp.1489~1501 (2005)
  19. 황윤정, "Karyomorphological Analysis of Wild Chrysanthemum boreale Collected from Four Natural Habitats in Korea", 화훼연구, 21(4), pp.182~189 (2013), 한국화훼학회[DOI]
  20. Lee YN, "A cytotaxonomic study on Chrysanthemum zawadskii complex in Korea (2) polyploidy", Journal of Plant Biology, 12, pp.35~48 (1969)
  21. Lema‐Rumiñska J, "Radiomutants of chrysanthemum (Dendranthema grandiflora Tzvelev) of the Lady group: RAPD analysis of the genetic diversity", Plant Breeding, 123, pp.290~293 (2004)
  22. Li H, "RNA-Seq derived identification of differential transcription in the chrysanthemum leaf following inoculation with Alternaria tenuissima", BMC Genomics, 15, pp.9~ (2014)
  23. Li P, "Genetic diversity, population structure and association analysis in cut chrysanthemum (Chrysanthemum morifolium Ramat.)", Molecular Genetics and Genomics, 291, pp.1117~1125 (2016)
  24. Li R-W, "The association analysis of phenotypic traits with SRAP markers in Chrysanthemum", Scientia Agricultura Sinica, 45, pp.1355~1364 (2012)
  25. Liu H, "Whole-transcriptome analysis of differentially expressed genes in the vegetative buds, floral buds and buds of Chrysanthemum morifolium", Plos One, 10, pp.e0128009~ (2015)
  26. Peng H, "Identification of quantitative trait loci for branching traits of spray cut chrysanthemum", Euphytica, 202, pp.385~392 (2015)
  27. Peng Y, "De novo genome assembly of the economically important weed horseweed using integrated data from multiple sequencing platforms", Plant Physiology, 166, pp.1241~1254 (2014)
  28. Ren L, "A transcriptomic analysis of Chrysanthemum nankingense provides insights into the basis of low temperature tolerance", BMC Genomics, 15, pp.1~ (2014)
  29. Ritter E, "Estimation of recombination frequencies and construction of RFLP linkage maps in plants from crosses between heterozygous parents", Genetics, 125, pp.645~654 (1990)
  30. Rogers K, "Biogenesis, turnover, and mode of action of plant microRNAs", Plant Cell, 25, pp.2383~2399 (2013)
  31. Wang HB, "Next-generation sequencing of the Chrysanthemum nankingense (Asteraceae)transcriptome permits large-scale unigene assembly and SSR marker discovery", Plos One, 8, pp.e62293~ (2013)
  32. Wang L, "Comparative transcriptome analysis of Chrysanthemum nankingense in response to nitrogen deficiency", Scientia Horticulturae, 195, pp.101~107 (2015)
  33. Wang Y, "In vitro conservation of chrysanthemum ‘Jinba’ and genetic stability of regenerated plantlets", Acta Botanica Boreali-Occidentalia Sinica, 27, pp.1341~1348 (2007)
  34. Wang Y, "Construction and de novo characterization of a transcriptome of Chrysanthemum lavandulifolium: analysis of gene expression patterns in floral bud emergence", Plant Cell, Tissue and Organ Culture, 116, pp.297~309 (2014)
  35. Xia X, "MicroRNA expression profile during aphid feeding in chrysanthemum (Chrysanthemum morifolium)", Plos One, 10, pp.e0143720~ (2015)
  36. Xia X, "Gene expression profiles responses to aphid feeding in chrysanthemum (Chrysanthemum morifolium)", BMC Genomics, 15, pp.1050~ (2014)
  37. Xu YJ, "Transcriptome sequencing and whole genome expression profiling of chrysanthemum under dehydration stress", BMC Genomics, 14, pp.15~ (2013)
  38. Yang WH, "Molecular evidence for multiple polyploidization and lineage recombination in the Chrysanthemum indicum polyploid complex (Asteraceae)", New Phytologist, 171, pp.875~886 (2006)
  39. Zhang F, "Detection of quantitative trait loci for leaf traits in chrysanthemum", Journal of Horticultural Science and Biotechnology, 87, pp.613~618 (2012)
  40. Zhang F, "A preliminary genetic linkage map of chrysanthemum (Chrysanthemum morifolium) cultivars using RAPD, ISSR and AFLP markers", Scientia Horticulturae, 125, pp.422~428 (2010)
  41. Zhang F, "SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium)", Molecular Breeding, 27, pp.11~23 (2011)
  42. Zhang F, "Genetic mapping of quantitative trait loci underlying flowering time in Chrysanthemum (Chrysanthemum morifolium)", Plos One, 8, pp.e83023~ (2013)
  43. Zhang F, "Mapping single-locus and epistatic quantitative trait loci for plant architectural traits in chrysanthemum", Molecular Breeding, 30, pp.1027~1036 (2012)
  44. Zhang F, "Identification of microRNAs and their targets associated with embryo abortion during chrysanthemum cross breeding via high-throughput sequencing", Plos One, 10, pp.e0124371~ (2015)
  45. Zhang F, "Transcriptomic and proteomic analysis reveals mechanisms of embryo abortion during chrysanthemum cross breeding", Scientific Reports, 4, pp.6536~ (2014)
  46. Zhang YS, "Application of genomic SSR Locus polymorphisms on the identification and classification of chrysanthemum cultivars in China", Plos One, 9, pp.e104856~ (2014)
  47. Zhao HE, "Chrysanthemum genetic resources and related genera of Chrysanthemum collected in China", Genetic Resources and Crop Evolution, 56, pp.937~946 (2009)

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