갯봄맞이(Glaux maritima L.)의 캘루스 배양을 통한 식물체 재분화
Plant regeneration via callus culture of sea-milkwort (Glaux maritima L.)
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
A callus-mediated regeneration protocol for sea-milkwort, an endangered coastal plant species in South Korea, is reported here. The explants of in vitro-plantlets generated from a node culture revealed distinguishable responses in callus induction depending on genotype, explant source, light condition, and 2,4-D concentration. Especially, continuous darkness exclusively facilitated callus induction from explants prior to other treatments. The calli initiated on the media with 2,4-D ranging from 0.1 mg/L to 3.0 mg/L in the dark vigorously proliferated when subcultured on the same media in continuous darkness. Given 1.0 mg/L zeatin in addition to darkness to the calli of the ‘Pistachio’ genotype, normal adventitious shoots were only regenerated from nodular structures that formed earlier from the calli at the frequency of 24.4 percent. Regenerated shoots easily grew into plantlets with roots and green color on a phytohormone-free MS medium under lighted condition, that were used for node culture as plant materials. Node culture effectively multiplied plantlets in accordance with protocol by Bae et al. (2016). Acclimatized plantlet clusters developed mature plant clusters under inland environment, followed by flowering the following April. Results were merged with node culture protocol suggested by Bae et al. (2016), which, as an in vitro propagation system for sea-milkwort, may contribute to natural habitat restoration.
Abstract
A callus-mediated regeneration protocol for sea-milkwort, an endangered coastal plant species in South Korea, is reported here. The explants of in vitro-plantlets generated from a node culture revealed distinguishable responses in callus induction depending on genotype, explant source, light condition, and 2,4-D concentration. Especially, continuous darkness exclusively facilitated callus induction from explants prior to other treatments. The calli initiated on the media with 2,4-D ranging from 0.1 mg/L to 3.0 mg/L in the dark vigorously proliferated when subcultured on the same media in continuous darkness. Given 1.0 mg/L zeatin in addition to darkness to the calli of the ‘Pistachio’ genotype, normal adventitious shoots were only regenerated from nodular structures that formed earlier from the calli at the frequency of 24.4 percent. Regenerated shoots easily grew into plantlets with roots and green color on a phytohormone-free MS medium under lighted condition, that were used for node culture as plant materials. Node culture effectively multiplied plantlets in accordance with protocol by Bae et al. (2016). Acclimatized plantlet clusters developed mature plant clusters under inland environment, followed by flowering the following April. Results were merged with node culture protocol suggested by Bae et al. (2016), which, as an in vitro propagation system for sea-milkwort, may contribute to natural habitat restoration.
참고문헌 (23)
- APG III, "An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants:APG III", Bot J Linn Soc, 161, pp.105~121 (2009)
- 배수지, "갯봄맞이(Glaux maritima L.) 실생의 단마디배양을 통한 기내증식", 원예과학기술지, 34(3), pp.461~471 (2016), 한국원예학회[DOI]
- Bunn E, "Biotechnology for saving rare and threatened flora in a biodiversity hotspot", Vitro Cell Dev Biol-Plant, 47, pp.188~200 (2011)[DOI]
- Compton ME, "Dark pretreatment improves adventitious shoot organogenesis from cotyledons of diploid watermelon", Plant Tissue Organ Cult, 58, pp.185~188 (1999)
- Curuk S, "hypocotyls of Cucumis species produces almost exclusively diploid shoots, and does not require light", HortScience, 38, pp.105~109 (2003)
- De Souza TV, "Morpho-histodifferentiation of Billbergia nodular cultures", Protoplasma, 254, pp.435~443 (2017)
- Farhadi N, "Effects of explant type, growth regulators and light intensity on callus induction and plant regeneration in four ecotypes of Persian shallot (Allium hirtifolium)", Sci Hort, 218, pp.80~86 (2017)
- Han, JS, "Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.)", Plant Cell Rep, 23, pp.291~296 (2004)
- Ikeuch M, "Plant callus: Mechanisms of induction and Repression", Plant Cell, 25, pp.3159~3173 (2013)
- "Threatened species in past and present IUCN Red Lists: 2015, The IUCN Red List of Threatened Species TM 2-2015: Summary statistics" (2015)[URL]
- Jerling, L., "Clone dynamics, population dynamics and vegetation pattern of Glaux maritima on a Baltic sea shore meadow", Vegetatio, 74, pp.171~185 (1988)
- Jerling, L, "Population dynamics of Glaux maritima L. along a distributional cline", Vegetatio, 74, pp.161~170 (1988)
- Leshem B, "Cytokinin and white light coact to enhance polypeptide metabolism and shoot regeneration in cultured melon cotyledons", J Plant Physiol, 145, pp.291~295 (1995)
- Murashige T, "A revised method for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.472~497 (1962)
- National Institute of Biological Resources, "Korean Red List of Threatened Species-Vascular plants", Republic of Korea, ~ (2012)
- Pence VC, "Evaluation costs for the in vitro propagation and preservation of endangered plants", Vitro Cell Dev Biol-Plant, 47, pp.176~187 (2011)
- Pence VC, "In vitro methods and the challenge of exceptional species for target 8 of the global strategy for plant conservation", Ann Miss Bot Gard, 99, pp.214~220 (2013)[DOI]
- Raghavan, V, "Role of 2,4-dichlorophenoxyacetic acid (2,4-D)in somatic embryogenesis on cultured zygotic embryos of Arabidopsis: cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2,4-D", American J Bot, 91, pp.1743~1756 (2004)
- Sahai A, "Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae)", Austrian J Bot, 58, pp.198~205 (2010)
- Scalzo J, "Effect of genotype, medium and light on in vitro plant proliferation of Vaccinium spp", New Zealand J Crop Hortic Sci, 44, pp.231~246 (2016)
- 손성원, "Distribution of five rare plants in Korea", 식물분류학회지, 41(3), pp.280~286 (2011), 한국식물분류학회
- Tomes DT, "The effect of parental genotype on initiation of embryogenic callus from elite maize (Zea mays L.)germplasm", Theor Appl Gen, 70, pp.505~509 (1985)
- Yokoyama T, "Adventitious bud formation through nodule induction by thidiazuron in cultured leaf segments of the Japanese persimmon (Diospyros kaki Thunb.)", Plant Biotech, 28, pp.339~344 (2011)