쉽싸리의 기내 마디배양 시 탄소급원의 종류와 농도가 식물체 재분화 및 증식에 미치는 영향
Effect of carbon source and concentration on in vitro regeneration and propagation in Lycopus lucidus by node culture
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초록
We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.
Abstract
We examined the effect of carbon sources on the regeneration and ex vitro acclimatization of Lycopus lucidus Turcz. ex Benth. Plantlets were regenerated on the 1/2MS medium supplemented with different concentrations (3 ~ 10%) of sucrose and glucose. The sucrose concentrations of 3% and 5% that were supplied enhanced shoot multiplication and rooting but hampered high concentration growth (including the length of the shoot and root). During ex vitro acclimatization, the tuberization of the root, the root length, the shoot length and the survival rate of Lycopus lucidus plantlets grown using 3% and 5% sucrose were found to be better than the other carbon sources and concentrations. Thus a sucrose concentration of 3% and 5% in the 1/2MS medium appeared to be better for both in vitro growth and ex vitro acclimatization of Lycopus lucidus.
참고문헌 (31)
- Ahmad T, "Comparison of sucrose and sorbitol as main carbon energy source in morphogenesis of peach rootstock GF-677", Pak J Bot, 39, pp.1264~1275 (2007)
- Baier M, "Characterization of mutants in Arabidopsis showing increased sugar-specific gene expression, growth, and developmental responses", Plant Physiol, 134, pp.81~91 (2004)[DOI]
- Butcher DN, "Excised root culture", Bot Rev, 30, pp.513~586 (1964)[DOI]
- Chen XJ, "Polysaccharide extract from Russula and its role of lowering blood glucose and lipid", Food Science, 31, pp.255~258 (2010)
- 최호진, "Ethanol Extract of Lycopus lucidus Elicits Positive Inotropic Effect Via Activation of Ca21 Entry and Ca21 Release in Beating Rabbit Atria", Journal of Medicinal Food, 16(7), pp.633~640 (2013), 한국식품영양과학회[DOI]
- Donnelly DJ, "Leaf anatomy of red raspberry transferred from culture to soil", J Amer Soc Hort Sci, 109, pp.177~181 (1984)
- Hazarika B N, "Acclimatization of tissue-cultured plants", Curr Sci, 85, pp.1704~1712 (2003)
- Hussain MS, "Current approaches toward production of secondary plant metabolites", J Pharm Bioallied Sci, 4, pp.10~20 (2012)
- Izabela GK, "The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpine", Plant Cell Tissue Organ Cult, 122, pp.699~708 (2015)[DOI]
- Jaime A, "The effect of carbon source on in vitro organogenesis of chrysanthemum thin cell layers", Bragantia, 63(2), pp.165~177 (2004)[DOI]
- Kebrom TH, "Transcriptome profiling of tiller buds provides new insights into PhyB regulation of tillering and indeterminate growth in sorghum", Plant Physiol, 170, pp.2232~2250 (2016)[DOI]
- 김지아, "탄소급원처리에 의한 기내 더덕 식물체의 비대근 형성", Journal of Plant Biotechnology, 40(3), pp.147~155 (2013), 한국식물생명공학회
- Koch K, "Carbohydrate-modulated gene expression in plants", Annu Rev Plant Biol, 47, pp.509~540 (1996)[DOI]
- Lee YJ, "Lycopus lucidus inhibits high glucose-induced vascular inflammation in human umbilical vein endothelial cells", Vascul Pharmacol, 48, pp.38~46 (2008)[DOI]
- Lin CR, "Antioxidation effects of Lycopus lucidus polyscharides on aged mice induced by D-Galactose", Chinese Pharmacolol Bull, 29, pp.1440~1443 (2012)
- Lu YH, "Variation in nutritional compositions, antioxidant activity and microstructure of Lycopus lucidus Turcz. root at different harvest times", Food Chem, 183, pp.91~100 (2015)[DOI]
- MacGregor DR, "Root system architecture in Arabidopsis grown in culture is regulated by sucrose uptake in the aerial tissues", Plant Cell, 20, pp.2643~2660 (2008)[DOI]
- Mason MG, "Sugar demand, not auxin, is the initial regulator of apical dominance", PNAS, 111, pp.6092~6097 (2014)[DOI]
- Muller B, "Spatial distributions of tissue expansion and cell division rates are related to irradiance and to sugar content in the growing zone of maize roots", Plant Cell Environ, 21, pp.149~158 (1998)[DOI]
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Plant, 15, pp.473~497 (1962)[DOI]
- Shin TY, "Anti-allergic effects of Lycopus lucidus on mast cell-mediated allergy model", Toxicol Appl Pharmacol, 209, pp.255~262 (2005)[DOI]
- Thomas BR, "Metabolite signals regulate gene expression and source", sink relations in cereal seedlings, 1994, pp.106, 1235~1239
- Thorpe C, "Chapter 4. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems;Plant propagation by tissue culture 3rd ed.", pp.115~174 (2007), Springer
- Xiong W, "Hypoglycemic and hypolipidemic effect of polysaccharide from wild plant Lycopus lucidus Turcz. on alloxan-induced diabetic mice", Chin J Bioprocess Eng, 9, pp.45~48 (2011)
- Xiong W, "Effects of polysaccharide from Lycopus lucidus Turcz. on proliferation of tumor cells in vitro and in vivo", Chin J Bioprocess Eng, 4, pp.64~69 (2012)
- Yang X, "Isolation, Characterization, and Immunological Effects of <TEX>$\alpha$<", TEX>-Galacto-oligosaccharides from a New Source, the Herb Lycopus lucidus Turcz, 2010, pp.58, 8253~8258
- Yao Y, "The aqueous extract of Lycopus lucidus Turcz ameliorates streptozotocin-induced diabetic renal damage via inhibiting TGF-signaling pathway", Phytomedicine, 20, pp.1160~1167 (2013)[DOI]
- Yaseen M, "Review: role of carbon sources for in vitro plant growth and development", Mol Biol Rep, 40, pp.2837~2849 (2013)[DOI]
- Yildiz M, "Sucrose effects on phenolic concentration and plant regeneration from sugarbeet leaf and petiole explants", J Sugar Beet Res, 44, pp.1~15 (2007)[DOI]
- Yu JQ, "Anticancer, antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz", Food Chem, 126, pp.1593~1598 (2011)[DOI]
- Zhou JG, "Tissue culture study of Lycopus lucidus", Journal of Henan Agricultural University, 37, pp.266~269 (2003)
