절간배양 방법을 이용한 감초의 기내 유식물체 재분화 및 순화
Acclimatization and Plants Regeneration of Mongolian Licorice (Glycyrrhiza uralensis Fisch.) using Nodal In vitro Culture
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Background: Licorice (Glycyrrhiza uralensis Fisch.) multiplies vegetatively through cuttings, but for farming this process is considered too slow. Therefore, it is necessary to develop more efficient and sustainable methods for producing licorice and its medicinal constituents. Methods and Results: Nodal segments (1.0 ㎝ - 1 .5 ㎝) were removed from healthy licorice plants to provide explants for micropropagation. The most successful shoot height and multiplication occurred using 2.0 ㎎/ℓ BAP + 0.1 ㎎/ℓ IAA in MS media. This media also provided the highest regeneration frequency (77.3%) and shoot formation (3.5 shoots/explants) after 16 weeks of culture. When in vitro plants were supplemented with activated charcoal (1.0 g/ℓ) to reduce hyperhydricity, the survival rate improved to 90.0%. A treatment of 2.0 ㎎/ℓ NAA in half strength MS medium proved to be superior for improving rooting capacity, with a maximum of 100% rooting. After acclimatizing to room temperature, the micropropagated plantlets were transferred to a greenhouse mist room, and grown in perlite / vermiculite (1 : 1, v/v). There was a 93.3% survival rate after eight weeks. Conclusions: Our experiments confirm that in vitro growth and multiplication of plantlets with micropropagation can be optimized to mass produce healthy and vigorous Licorice plant.
Abstract
Background: Licorice (Glycyrrhiza uralensis Fisch.) multiplies vegetatively through cuttings, but for farming this process is considered too slow. Therefore, it is necessary to develop more efficient and sustainable methods for producing licorice and its medicinal constituents. Methods and Results: Nodal segments (1.0 ㎝ - 1 .5 ㎝) were removed from healthy licorice plants to provide explants for micropropagation. The most successful shoot height and multiplication occurred using 2.0 ㎎/ℓ BAP + 0.1 ㎎/ℓ IAA in MS media. This media also provided the highest regeneration frequency (77.3%) and shoot formation (3.5 shoots/explants) after 16 weeks of culture. When in vitro plants were supplemented with activated charcoal (1.0 g/ℓ) to reduce hyperhydricity, the survival rate improved to 90.0%. A treatment of 2.0 ㎎/ℓ NAA in half strength MS medium proved to be superior for improving rooting capacity, with a maximum of 100% rooting. After acclimatizing to room temperature, the micropropagated plantlets were transferred to a greenhouse mist room, and grown in perlite / vermiculite (1 : 1, v/v). There was a 93.3% survival rate after eight weeks. Conclusions: Our experiments confirm that in vitro growth and multiplication of plantlets with micropropagation can be optimized to mass produce healthy and vigorous Licorice plant.
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