가시없는 블랙베리(Rubus fruticosus L. cv. BB21)의 근맹아를 이용한 다경유도와 절간배양을 통한 식물체 증식조건의 확립
Establishment of optimal conditions for micropropagation by node culture and multiple shoots formation from sucker explants of thornless Blackberry (Rubus fruticosus L. cv. BB21)
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
This study was conducted to develop a simple, rapid, and reliable method for in vitro propagation of disease-free and true-to-type clones from sucker explants of thornless blackberry (Rubus fruticosus L. × R. parvifolius L.). To induce multiple shoots, the sucker explants were sterilized in 1% NaOCl solution, and then were aseptically cultured on the full and 1/2 MS solid medium supplemented with BAP (0.1, 0.5, 1.0, 2.0 mg/L). After six weeks of culture, the highest frequency (85.4%) of shoot formation from sucker explants was obtained on the full-strength MS medium with 1.0 mg/L BAP. Node explants obtained from multiple shoots were cultured on the various media of full- or half-strength of AD, B5, MS, SH, QL, WPM media, respectively. After 30 days of culture, plant growth was good on the half-AD, half-QL medium. After 90 days of culture, plant growth was good on the full MS and full SH medium. The survival rate of the plantlets after transfer to plastic pots containing soil mixture (sand: soil: vermiculite was 1:1:1, vol.) in the greenhouse was 98%. The results indicate that a multiple-shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. × R. parvifolius L.
Abstract
This study was conducted to develop a simple, rapid, and reliable method for in vitro propagation of disease-free and true-to-type clones from sucker explants of thornless blackberry (Rubus fruticosus L. × R. parvifolius L.). To induce multiple shoots, the sucker explants were sterilized in 1% NaOCl solution, and then were aseptically cultured on the full and 1/2 MS solid medium supplemented with BAP (0.1, 0.5, 1.0, 2.0 mg/L). After six weeks of culture, the highest frequency (85.4%) of shoot formation from sucker explants was obtained on the full-strength MS medium with 1.0 mg/L BAP. Node explants obtained from multiple shoots were cultured on the various media of full- or half-strength of AD, B5, MS, SH, QL, WPM media, respectively. After 30 days of culture, plant growth was good on the half-AD, half-QL medium. After 90 days of culture, plant growth was good on the full MS and full SH medium. The survival rate of the plantlets after transfer to plastic pots containing soil mixture (sand: soil: vermiculite was 1:1:1, vol.) in the greenhouse was 98%. The results indicate that a multiple-shoot procedure can be applied for an efficient mass propagation of Rubus fruticosus L. × R. parvifolius L.
참고문헌 (30)
- Anderson, "Tissue culture propagation of red and black raspberries (Rubus idaeus and R. occidentalis)", Acta Hort., 112, pp.13~20 (1980)
- Bobrowski VL, "Micropropagation of blackberries (Rubus sp.) cultivars", Rev. Bras. de Agrociencia, 2, pp.17~20 (1996)
- Broome OC, "In vitro propagation of blackberry", HortSci, 13, pp.151~153 (1978)
- Casto BC, "Chemoprevention of oral cancer by black rasberries", Anticancer Res., 22, pp.4005~4015 (2002)
- Cho MJ, "Flavonoid glycosides and antioxidant capacity of various blackberry, blueberry and red grape genotypes determined by high-performance liquid chromatography", mass spectrometry, 2004, pp.84(13), 771~1782
- Cuenca S, "Micropropagation from inflorescence stems of the Spanish endemic plant Centaurea paui Loscos ex Willk. (Compositae)", Plant Cell Rep, 18, pp.674~679 (1999)[DOI]
- Debnath SC, "Clonal propagation of dwarf raspberry (Rubus pubescens Raf.) through in vitro axillary shoot proliferation", Plant growth regul, 43, pp.179~186 (2004)[DOI]
- Fernandez GE, "In vitro propagation of the erect thornless 'Navaho' blackberry", Hortscience, 26(9), pp.12~19 (1991)
- Gamborg. O., "Nutrient Requirements of Suspension Cultures of Soybean Root Cells", Experimental Cell Research, (50), pp.151~158 (1968)
- 한무석, "멸종위기식물 피뿌리풀의 기내증식", Journal of Plant Biotechnology, 31(1), pp.31~35 (2004), 한국식물생명공학회
- Harper PC, "Tissue culture propagation of blackberry and tayberry", Hortic Res, 18, pp.141~143 (1978)
- 강호덕, "Peace 포플러(Populus koreana X P.trichocarpa)의 줄기형성에 미치는 Thidiazuron 효과", Journal of Plant Biotechnology, 31(1), pp.49~53 (2004), 한국식물생명공학회
- Kim KH, "Antioxidative activity of tannins from Rubus coreanum", Yakhak Hoeji, 44, pp.354~357 (2000)
- Kim MS, "Flavunoids from the leaves of Rubus coreanum", Yakhak Hoeji, 40, pp.666~669 (1997)
- Kim MS, "Tannins from the leaves of Rubus coreanum", Yakhak Hoeji, 40, pp.666~669 (1996)
- Kresty LA, "Transitioning from preclinical to clinical chemopreventive assessments of lyophilized blackberries: interim results show berries modulate markers of oxidative stress in Barrett's esophagus patients", Nutrition and Cancer, 54, pp.148~156 (2006)[DOI]
- Linsmaier EM, "Organic growth factor requirements of tobacco", Physiol. Plant, 18, pp.100~127 (1975)
- Lloyd G., "Connercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot-tip culture", Combined Proceeding of the International plant Propagation Society, 30, pp.421~426 (1981)
- Meng R, "Improving in vitro plant regeneration from leaf and petiole explants of 'Marion' blackberry", Hortscience, 39(2), pp.316~320 (2004)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Plant., 15, pp.473~497 (1962)[DOI]
- Quoirin M, "Improved medium for in vitro culture of Prunus sp", Acta Hortic, 78, pp.437~442 (1977)
- Schenk T, "Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures", Can J Bot, 50, pp.199~204 (1972)[DOI]
- 신정순, "교잡종 블랙베리(Rubus fruticosus L. × R. parvifolius L.)의근맹아 절편체로부터 다경유도를 통한 식물체 재분화", 한국자원식물학회지, 21(2), pp.111~116 (2008), 한국자원식물학회
- 신정순, "블랙베리(Rubus fruticosus L.)의 맹아절편체로부터 다경유도를 통한 식물체 재분화", 한국자원식물학회지, 18(3), pp.456~13 (2005), 한국자원식물학회
- Skirvin RM, "In vitro propagation of thornless trailing blackberries", Hortscience, 16(3), pp.310~312 (1981)
- Thompson MM, "Survey of chromosom number in Rubus (Rosaceae: Rosoideae)", Ann Mol Bot Garden, 84, pp.129~165 (1997)
- Turk BA, "Adventitious shoot regeneration from in vitro-cultured leaves of Rubus genotypes", Plant Cell Tiss Org cult, 38, pp.11~17 (1994)[DOI]
- Wang SY, "Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage", J Agr Food Chem, 48(2), pp.140~146 (2000)[DOI]
- Wu J-H, "Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus", Plant Cell Tiss Org, 99, pp.17~25 (2009)[DOI]
- Zawadzka M, "Increase in the quality of raspberry cultures under the influence of FeEDDHA", Acta Hortic, 725, pp.161~164 (2006)