블랙베리(Rubus fruticosus L.)의 맹아절편체로부터 다경유도를 통한 식물체 재분화

Abstract

This study was carried out to induce plant regeneration via shoot formation from suckerexplants of Rubus fruticosusL. To induce adventitious shoots, sucker explants weresterilized in 1.2% NaOCl solution, and cultured on the MS solid medium supplemented withkinetin (0.5, 1.0, 3.0 ㎎/L) and BA (0.5, 1.0, 3.0 ㎎/L), respectively. As above, to induceadventitious shoots, sucker explants were cultured on the MS solid medium supplementedwith IBA (0, 0.1, 1.0 ㎎/L) and BA (0, 0.1, 1.0, 2.0 ㎎/L). After 4 weeks of culture, the highestfrquency (100%) of shoot formation from sucker explants was obtained from the mediumwith 1.0 ㎎/L BA. The highest shoot number per explant from in vitro shoot explants was5.3. After 10 weeks of culture, the number of shoot per explant was increased. The highestfrequency (85%) of root formation was obtained at 0.5 ㎎/L glycine medium, when theexplant with shoot were cultured on the MS medium containing glycine at variousconcentrations from 0 to 2.0 ㎎/L. The survival rate of the plantlets after transfer to plasticpots containing sand, soil, and vermiculite (1:1:1, vol.) was 95%. The results indicate thatmicropropagation procedure can be applied for an efficient mass propagation of Rubusfruticosus.