경정배양을 통한 딸기 품종 ‘킹스베리’와 ‘하이베리’의 조직배양묘 대량생산
Mass production of tissue-cultured plantlets of strawberry cultivars ‘Kingsberry’ and ‘Highberry’ through shoot tip culture
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Research on the mass propagation of domestic strawberry cultivars through tissue culture remains insufficient, particularly concerning the in vitro proliferation rate, the occurrence of somaclonal variation, and their response to the types and concentrations of plant growth regulators. Therefore, this study aimed to determine the optimal combination of 6-benzylaminopurine (BAP) and coconut water (CW) for mass propagation with minimal somaclonal variation in tissue culture of the commercially available cultivars ‘Kingsberry’ and ‘Highberry’. Multishoots that served as experimental materials were initially produced from strawberry cultivars through meristem culture and shoot tip culture on medium supplemented with 2.2 μM BAP and 0.49 μM indole-3-butyric acid (IBA). These initial multishoots were then cultured for 10 weeks on ¾ Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (1, 2, 4, and 8 μM) and CW (0, 10, 25, 50, and 100 mL/L), resulting in 20 treatment combinations per cultivar with 10 replicates each. The two best combinations for ‘Kingsberry’ were BAP 1 μM with CW 0 mL/L (average 68.40 shoots) and BAP 1 μM with CW 100 mL/L (average 63.10 shoots). For ‘Highberry’, the two best combinations were BAP 1 μM with CW 50 mL/L (average 65.00 shoots) and BAP 1 μM with CW 100 mL/L (average 54.50 shoots). For each optimal combination, 50 multishoots were cultured on hormone-free medium, subsequently acclimatized, and subjected to progeny testing. To ensure genetic stability, mutation detection using molecular markers should be performed.
Abstract
Research on the mass propagation of domestic strawberry cultivars through tissue culture remains insufficient, particularly concerning the in vitro proliferation rate, the occurrence of somaclonal variation, and their response to the types and concentrations of plant growth regulators. Therefore, this study aimed to determine the optimal combination of 6-benzylaminopurine (BAP) and coconut water (CW) for mass propagation with minimal somaclonal variation in tissue culture of the commercially available cultivars ‘Kingsberry’ and ‘Highberry’. Multishoots that served as experimental materials were initially produced from strawberry cultivars through meristem culture and shoot tip culture on medium supplemented with 2.2 μM BAP and 0.49 μM indole-3-butyric acid (IBA). These initial multishoots were then cultured for 10 weeks on ¾ Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (1, 2, 4, and 8 μM) and CW (0, 10, 25, 50, and 100 mL/L), resulting in 20 treatment combinations per cultivar with 10 replicates each. The two best combinations for ‘Kingsberry’ were BAP 1 μM with CW 0 mL/L (average 68.40 shoots) and BAP 1 μM with CW 100 mL/L (average 63.10 shoots). For ‘Highberry’, the two best combinations were BAP 1 μM with CW 50 mL/L (average 65.00 shoots) and BAP 1 μM with CW 100 mL/L (average 54.50 shoots). For each optimal combination, 50 multishoots were cultured on hormone-free medium, subsequently acclimatized, and subjected to progeny testing. To ensure genetic stability, mutation detection using molecular markers should be performed.