에탄올 농도에 따른 돌외 잎 차 추출물의 생리활성
Physiological activity of the extract from Dolwoe (Gynostemma pentaphyllum Makino) leaves tea by different ethanol concentrations
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초록
The objective of this study was to compare the effects of different concentration of ethanol extraction on the total phenol and flavonoid contents and physiological activities. The total phenol content of the extracts ranged from 35.54 to 71.52 mg GAE/g. An increase in the ethanol concentration of the solvent led to an increase in the phenol content, with the highest content being found in the 80 and 99.5% ethanol extract. The same trend was observed for flavonoid content. DPPH, nitric oxide, superoxide, hydroxyl radical scavenging activity and TEAC, FRAP and ORAC were measured by antioxidant assay. Radical scavenging activity of aqueous ethanol extracts was better than that of water and 99.5% ethanol extracts. TEAC and FRAP were highly dependent on ethanol concentration and ORAC showed high activity in 40 ~ 80% ethanol extract. Antioxidant activity of Dolwoe leaves tea showed different results among the assay systems. In most experiments, the activities of water and 99.5% ethanol extracts was relatively low. α- Glucosidase inhibitory activity and microorganism inhibitory activity were highest in the 80% and 99.5% ethanol extracts. Therefore, it was considered that extraction with 80% ethanol was appropriate when considering the antioxidative and physiological activities of Dolwoe leaves tea. Based on these results, it can be used as a basic data for the development of food of Dolwoe leaves tea.
Abstract
The objective of this study was to compare the effects of different concentration of ethanol extraction on the total phenol and flavonoid contents and physiological activities. The total phenol content of the extracts ranged from 35.54 to 71.52 mg GAE/g. An increase in the ethanol concentration of the solvent led to an increase in the phenol content, with the highest content being found in the 80 and 99.5% ethanol extract. The same trend was observed for flavonoid content. DPPH, nitric oxide, superoxide, hydroxyl radical scavenging activity and TEAC, FRAP and ORAC were measured by antioxidant assay. Radical scavenging activity of aqueous ethanol extracts was better than that of water and 99.5% ethanol extracts. TEAC and FRAP were highly dependent on ethanol concentration and ORAC showed high activity in 40 ~ 80% ethanol extract. Antioxidant activity of Dolwoe leaves tea showed different results among the assay systems. In most experiments, the activities of water and 99.5% ethanol extracts was relatively low. α- Glucosidase inhibitory activity and microorganism inhibitory activity were highest in the 80% and 99.5% ethanol extracts. Therefore, it was considered that extraction with 80% ethanol was appropriate when considering the antioxidative and physiological activities of Dolwoe leaves tea. Based on these results, it can be used as a basic data for the development of food of Dolwoe leaves tea.
참고문헌 (27)
- An BJ, "Effect of inhibition on glucosyltransferase and antimicrobial activity of polyphenol fraction of gallnut and red grape husk", Korean J Food Preserv, 8, pp.217~223 (2001)
- Althaus JS, "Structure activity relationships of peroxynitrite scavengers an approach to nitric oxide neurotoxicity", Res Commun Chem Patho Pharmacol, 83, pp.243~254 (1994)
- Beckman KB, "The free radical theory of aging matures", Physiol Rev, 78(2), pp.547~581 (1998)
- Benzie IFF, "The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay", Anal Bio Chem, 39, pp.70~76 (1996)
- Blois MS, "Antioxidant determinations by use of a stable free radical", Nature, 181, pp.1199~1200 (1958)
- Halliwell B, "DNA damage by oxygen-derived species. Its mechanism and measurement in mammalian systems", FEBS Lett, 281, pp.9~19 (1991)
- Huang WY, "Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention", Nutr cancer, 62(1), pp.1~20 (2009)
- 황초롱, "열처리 온도에 따른 더덕과 도라지의 화학성분과 항산화활성", 한국식품영양과학회지, 40(6), pp.798~803 (2011), 한국식품영양과학회
- 김주성, "팔리다자주닭개비 잎 추출물 및 분획물의 항산화 및 α-글루코시다아제 저해 활성", 한국약용작물학회지, 24(3), pp.222~227 (2016), 한국약용작물학회[DOI]
- 김경철, "돌외 잎 추출물의 항산화 활성에 미치는 에탄올 용매 농도의 영향", 공학기술논문지, 11(3), pp.197~203 (2018), 공학기술연구원[DOI]
- Kim YE, "in components and sensory attribute of the oil extracted from perilla seed roasted at different roasting conditions", Agri Chem Biotech, 39, pp.118~122 (1996)
- 김연숙, "산국대 추출물의 항산화 활성 및 간세포 보호 효과", 한국식품영양과학회지, 43(1), pp.60~66 (2014), 한국식품영양과학회
- 고현민, "서양금혼초 부위별 추출물의 티로신에이즈, α-글루코시다아제 저해활성 및 항산화효과", 한국약용작물학회지, 25(3), pp.139~145 (2017), 한국약용작물학회[DOI]
- Lee BW, "Screening of natural antimicrobial plant extract on food spoilage microorganisms", Korean J Food Sci Technol, 23(2), pp.200~204 (1991)
- 이정민, "마전자 추출물의 항산화 및 Alpha-Glucosidase 저해 활성", 한국식품영양과학회지, 39(9), pp.1243~1248 (2010), 한국식품영양과학회
- Liu J, "Anticancer and immunoregulatory activity of Gynostemma pentaphyllum polysaccharides in H22 tumor-bearing mice", Int J Biol Macromol, 69, pp.1~4 (2014)
- Lu Y, "Gypenosides altered hepatic bile acids homeostasis in mice treated with high fat diet", Evid Based Complement Alternat Med (2018)
- Megalli S, "Anti-hyperlipidemic and hypoglycemic effects of Gynostemma pentaphyllum in the Zucker fatty rat", J Pharm Pharm Sci, 9(3), pp.281~291 (2006)
- Mitscher LA, "Antimicrobial agents from higher plants. Antimicrobial isoflavanoids and related substances from Glycyrrhiza glabra L. var. typica", J Nat Prod, 43(2), pp.259~269 (1980)
- Moon GS, "Comparison of antioxidative activities of soybean components by different assays", Korea Soybean Digest, 20(1), pp.28~36 (2003)
- Ratnam DV, "Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective", J Control Release, 113(3), pp.189~207 (2006)
- Rice-Evans C, "Antioxidant properties of phenolic compounds", Trends Plant Sci, 2(4), pp.152~159 (1997)
- Ryu KD, "Optimization of roasting conditions for high-quality Polygonatum odoratum tea", Korean J Food Sci Technol, 29, pp.776~783 (1997)
- 원범영, "에탄올 농도에 따른 미나리 추출물의 영양성분 변화", 한국식품영양과학회지, 44(6), pp.882~887 (2015), 한국식품영양과학회
- 허명록, "토후박 추출물의 항산화 및 항당뇨 활성", 한국약용작물학회지, 18(1), pp.34~39 (2010), 한국약용작물학회
- Xu YQ, "Effect of extraction methods on the chemical components and taste quality of green tea extract", Food Chem, 248, pp.146~154 (2018)
- Zulueta A, "ORAC and TEAC assays comparison to measure the antioxidant capacity of food products", Food Chem, 114, pp.310~316 (2009)