본문 바로가기
KERD
논문KCI 등재

철피석곡의 기내 Protocorm Like Bodys(PLBs) 재증식 및 신초형성에 미치는 생 장조절제 및 탄소원의 영향

Effect of plant growth regulators and carbon sources on proliferation and shoot formation of PLBs in Dendrobium candidum

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류농학

초록

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocormlike bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in H3P4 basal medium containing 0.1 mg · L-1 NAA and 0.1 mg · L-1 Kinetin. PLB growth was the best when 10 g · L-1 sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or H1P2 medium containing 10 g · L -1 sucrose, and the shoot length was longer than the others.

Abstract

Dendrobium candidum Wallich ex Lindley is a traditional Chinese medicine plant and has been widely used for medicinal and ornamental purposes. In this study, several different factors affecting micro propagation of protocormlike bodies (PLBs) such as basal media, plant growth regulators, and carbon sources. The proliferation PLB derived from seeds was the best in H3P4 basal medium containing 0.1 mg · L-1 NAA and 0.1 mg · L-1 Kinetin. PLB growth was the best when 10 g · L-1 sucrose was added to the carbon atoms in the medium. The rate of shoot formation from the propagated PLB was the highest in 1/4 MS or H1P2 medium containing 10 g · L -1 sucrose, and the shoot length was longer than the others.

참고문헌 (41)

  1. 배기화, "멸종위기 수종 석곡(Dendrobium moniliforme)의 Protocorm-like Body 형성 및 기내증식", Journal of forest and environmental science, 30(1), pp.126~132 (2014), 산림과학연구소[DOI]
  2. Chen B, "Micropropagation of the endangered medicinal orchid, Dendrobium officinale", Life Sci J, 11, pp.526~530 (2014)
  3. Chen JT, "Plant regeneration via embryo and shoot formation from flower-stalk explants of Oncidium ‘Sweet Sugar’", Plant Cell Tissue Organ Cult, 62, pp.95~100 (2000)
  4. Chen W, "In vitro rapid propagation of stems of Dendrobium candicum", Plant Physiology Communications, 38, pp.145~145 (2002)
  5. Chen YC, "Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis", Plant Growth Regu, 16, pp.99~101 (1995)
  6. 崔海燕, "생물반응기 시스템을 이용한 Dendrobium candidum PLB(Protocorm-like body)의 대량증식과 생리활성물질 생산", 박사, 원예학과 원예학전공, pp.2012
  7. Cui HY, "Biomass and polysaccharide production in PLB and multiple shoot culture of Dendrobium candidum: Effect of different hyponex media", Korean J Hortic Sci Technol, 29, pp.175~176 (2011)
  8. Cui HY, "Protocorm culture of Dendrobium candidum in balloon type bubble bioreactors", Biochem Eng J, 88, pp.26~29 (2014)
  9. Da Silva JAT, "Priming abiotic factors for optimal hybrid Cymbidium (Orchidaceae)PLB and callus induction, plantlet formation, and their subsequent cytogenetic stability analysis", Sci Hortic, 109, pp.368~378 (2006)
  10. He JB, "Multiplication of protocorm of Dendrobium candidum", Acta Bot Yunnanica, 4, pp.211~212 (1982)
  11. Jones WE, "Nuclear DNA content of 26 orchid (Orchidaceae) genera with emphasis on Dendrobium", Ann Bot, 82, pp.189~194 (1998)
  12. Kim JH, "production system in vitro phalaenopsis plants and problems on domestic tissue culture facilities in Korea", 1th Orchid symposium, pp.11~19 (2010)
  13. Kim MS, "Effect of culture medium, temperature, and light intensity on PLB propagation of Phalaenopsis", Korean J Plant Tissue Culture, 28, pp.215~219 (2001)
  14. Kim TJ, "Effect of different shading on CO2 uptake and growth and development of Dendrobium phalaenopsis", Korean J Hortic Sci Technol, 42, pp.111~115 (2001)
  15. Kusumoto M, "Effects of coconut milk, agar, and sucrose concentrations, and media pH on the proliferation of Cymbidium protocorm-like bodies cultured in vitro", J Jpn Soc Hortic Sci, 48, pp.503~509 (1980)
  16. Li B, "Effect of sound wave stress on antioxidant enzyme activities and lipid peroxidation of Dendrobium candidum", Colloids Surf B:Biointerfaces, 63, pp.269~275 (2008)
  17. Li Y, "Chemical constituents of Dendrobium candidum", Zhongguo Zhong Yao Za Zhi, 35, pp.1715~1719 (2010)
  18. Li Y, "Three new bibenzyl derivatives from Dendrobium candidum", Chem Pharm Bull, 57, pp.218~219 (2009)
  19. Liu W, "A method for isolating functional RNA from callus of Dendrobium candidum contented rich polysaccharides", Colloids Surf B Biointerfaces, 42, pp.259~262 (2005)
  20. Martin KP, "In vitro propagation of Dendrobium hybrids using flower stalk node explants", Indian J Exp Biol, 43, pp.280~285 (2005)
  21. Murashige T, "A revised medium for rapid growth and bio assays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
  22. Nambiar N, "Effects of organic additives and different carbohydrate sources on proliferation of protocorm like bodies in Dendrobium Alya Pink", POJ, 5(1), pp.10~18 (2012)
  23. Ng CY, "In vitro propagation of Paphiopedilum orchid through formation of protocorm-like bodies", Plant Cell Tissue Organ Cult, 105, pp.193~202 (2011)
  24. 박창민, "철피석곡의 원괴체상구체 및 다신초 추출물의 생리활성에 관한 비교 연구", 대한화장품학회지, 40(1), pp.29~36 (2014), 사단법인 대한화장품학회[DOI]
  25. Park SY, "Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis", Plant Sci, 164, pp.919~923 (2003)
  26. Park YS, "Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium", Plant Cell Tissue Organ Cult, 45, pp.79~85 (1996)
  27. 노희선, "다양한 배지종류, sucrose 농도 및 갈변억제물질 처리에 의한 팔레놉시스 PLB 증식 및 재분화 체계확립", Journal of Plant Biotechnology, 41(4), pp.223~228 (2014), 한국식물생명공학회
  28. Shiau YJ, "In vitro propagation of the Chinese medicinal plant, Dendrobium candidum Wall. Ex Lindl., from axenic nodal segments", In Vitro Cell Dev Biol, Plant, 41, pp.666~670 (2005)
  29. Shimura H, "Micropropagation of Cypripedium macranthos var. rebunense through protocorm-like bodies derived from mature seeds", Plant Cell Tissue Organ Cult, 78, pp.273~276 (2004)
  30. Su H, "State quo of Dendrobium spp. resources of nabanhe nature reserve and countermeasures for protection", For Invent Plann, 31, pp.100~102 (2006)
  31. Sun YY, "Current situation and development countermeasures of Dendrobium spp. industry for medicinal purpose of Yunnan province", For Invent Plann, 31, pp.45~47 (2006)
  32. Udomdee W, "Effect of carbon source on protocorm-like body induction, proliferation and regeneration in Dendrobium snowfalke ‘RED STAR’", Acta Hortic, 1078, pp.113~120 (2015)
  33. Vacin E F, "Some pH changes in nutrient solutions", Botanical Gazette, 110, pp.605~613 (1949)
  34. Ye XL, "Morphology of immature seeds and development in vitro of Dendrobium candidum", Acta Bot Yunnanica, 10, pp.285~290 (1988)
  35. Zha XQ, "Enhancement of polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense by optimization of medium compositions and feeding of sucrose", Process Biochem, 42, pp.344~351 (2007)
  36. Zhang Q, "Tissue culture and in vitro seedling and protocorm-like body examination of Dendrobium candidum", Xibei Zhiwu Xuebao, 25, pp.1761~1765 (2004)
  37. Zhao P, "High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl", Plant Cell Tissue Organ Cult, 90, pp.131~139 (2007)
  38. Zhao P, "Protocorm-like body (PLB) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl", In Vitro Cell Dev Biol, Plant, 44, pp.178~185 (2008)
  39. Xin Zhao, "D. candidum has in vitro anticancer effects in HCT-116 cancer cells and exerts in vivo anti-metastatic effects in mice", Nutrition Research and Practice, 8(5), pp.487~493 (2014), 한국영양학회
  40. Zhou G, "Factors Affect the growth of Dendrobium canducum Wall. ex Lindl. in Vitro", Jiangxi Science, 17, pp.231~235 (1999)
  41. Zhu Y, "Quantitive variation of polysaccharides content in cultivated Dendrobium candidum", Zhongguo Zhong Yao Za Zhi, 35, pp.427~430 (2010)

관련 논문AI추천

이 간행물의 다른 논문 (7건)