발아콩에서 대사유도물질 처리에 의한 isoflavone 대사 관련 유전자 IFS와 IFR의 발현 양상과 isoflavone 생합성 변화
Manipuation of Isoflavone Levels in Sobyean Sprouts and Expression Pattern of Two Genes Coded for Isoflavone Reductase and Isoflavone Synthase by Chemical Treatments
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Abstract
Isoflavone is synthesized in several different organs such as root, leaf and developing and germinating seed. Isoflavone content reflects overall history of physiological event given to a plant during certain period of time. Amount of isoflavone in a organ or tissues is controlled by two key enzymes of isoflavone metabolism, isoflavone synthase and isoflavone reductase, ultimately. Many genes engaged in isoflavone synthesis response to biotic and abiotic stresses. In our previous study, it appeared that isoflavone content in germinating mung bean seed is increased by stress related chemical compound, for example, salicylic acid. Here, we found that both genes encoding isoflavone synthase and isoflavone reductase are over-expressed in germinating soybean seed by salicylic and acetyl salicylic acid treatment. These over-expression of both genes led to enhenced level of isoflavone in germinating soybean seed.
참고문헌 (21)
- "Single-step method ofRNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", pp.156~159
- Dixon RA, "Stress induced phenylpro-panoid metabolism", 7, pp.1085~1097 (1995)
- He XZ, "Stress responsesin alfalfa (Medicago sativa L). XII. cDNA cloning andcharacterization of an elicitor-inducible isoflavone 7-O-methyltransferase", 36(1), pp.43~54 (1998)
- Jung W, "Identification and expressionof isoflavone synthase, the key enzyme for biosynthesisof isoflavones in legumes", 18(2), pp.208~212 (2000)
- Kim EM, "Comparison in iso-flavone contents between soybean and soybean sproutsof soybean cultivas", 37(1), pp.45~51 (2004)
- Kwon CS, "Effect of soybean supple-mentation on murine drug-metabolizing enzymes andbenzo(a)pyrene-induced lung cancer development", 31, pp.535~539 (1999)
- Kwon CS, "Effects of soyisoflavone-supplemented iets on benzo pyrene-inducedlung and forestomach tumorigenesis in mice", 9(4), pp.249~252 (2000)
- Ludwig A, "Defence gene exp-ression in soybean is linked to the status of the celldeath", 4, pp.209~218 (2000)
- Mackowiak PA, "Brief history of antipyretic therapy", 5, pp.154~156
- Messina MJ, "A review of invitro and indata", pp.113~131
- Moon BK, "Isoflavone Contentsin Some Varieties of Soybean and on Procesing Conditions", 12, pp.527~535 (1996)
- Morrissey JP, "Fungal Resistance toPlant Antibiotics as a Mechanism of Pathogenesis", 63(3), pp.708~724 (1999)
- Oh HS, "Isoflavone contents,Antoxidative and Fibrinolylic Activities of Red Beanand Mung Bean", 19(3), pp.263~270 (2003)
- Pieterse CM, "A novel signalingpathway controlling induced systemic resistance in Ara-bidopsis", 10(9), pp.1571~1580 (1998)
- Rohrdanz E, "Thephytoestrogen daidzein afects the antioxidant enzymesystem of rat hepatoma H4IIE cells", 132(3), pp.370~375 (2002)
- Schenk PM, "Coordinated plantdefense responses in Arabidopsis revealed by microarrayanalysis", pp.11655~11660
- Sohn HS, "Doessoybean isoflavone have adverse ffects on Human", 17(2), pp.9~19 (2000)
- Su SJ, "The potentialof soybean foods as a chemoprevention approach for humanurinary tract cancer", 6(1), pp.230~236 (2000)
- Suzuki H, "Methyl jasmonate and yeast elicitorinduce differential transcriptional and metabolic re-pro-gramming in cell suspension cultures of the modellegume Medicago truncatula", 220(5), pp.696~707 (2005)
- Tastuta M, "Atenuation by genistein of sodium-chroride-enhanced gastric carcinogenesis induced byN-methyl-N-nitro-N-nitrosoguanidine in Wistar rats", 80(3), pp.396~399 (1999)
- Thomma BPHJ, "Separate jasmonate-dependent and salicylate-dependentdefense-response pathways in Arabidopsis are ssentialfor resistance to distinct microbial pathogens", 95, pp.15107~15111