Genome-wide identification of oleosin family and expression analysis in response to abiotic stresses in balloon flower ( Platycodon grandiflorus )
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Oleosins (OLEs) are structural proteins commonly found within oil bodies (OBs), playing a significant role in regulating the number, size, and stability of OBs. Therefore, this study aims to comprehensively analyze the OLE family in balloon flower (Platycodon grandiflorus) - a significant medicinal plant. Through genome-wide exploration and bioinformatics analyses, we identified and characterized five putative OLE proteins from P. grandiflorus (PlgOLEs). This study provides a comprehensive overview of this gene family in balloon flowers, including phylogenic analysis, conserved motifs, isoelectric points, and hydrophobicity. The study revealed the presence of central hydrophobic regions with a proline knot motif, a characteristic feature shared by OLE proteins in various plant species. Additionally, tissue-specific expression analysis revealed that PlgOLEs were predominantly expressed in seeds, indicating their crucial role in seed OB stability. Furthermore, expression profiling under abiotic stresses demonstrated that PlgOLEs are stress-inducible genes, suggesting their diverse physiological functions in stress responses. These findings shed light on the functional diversity of OLEs in balloon flowers and establish a basis for further research, including genetic modification studies, to elucidate their physiological roles.
Abstract
Oleosins (OLEs) are structural proteins commonly found within oil bodies (OBs), playing a significant role in regulating the number, size, and stability of OBs. Therefore, this study aims to comprehensively analyze the OLE family in balloon flower (Platycodon grandiflorus) - a significant medicinal plant. Through genome-wide exploration and bioinformatics analyses, we identified and characterized five putative OLE proteins from P. grandiflorus (PlgOLEs). This study provides a comprehensive overview of this gene family in balloon flowers, including phylogenic analysis, conserved motifs, isoelectric points, and hydrophobicity. The study revealed the presence of central hydrophobic regions with a proline knot motif, a characteristic feature shared by OLE proteins in various plant species. Additionally, tissue-specific expression analysis revealed that PlgOLEs were predominantly expressed in seeds, indicating their crucial role in seed OB stability. Furthermore, expression profiling under abiotic stresses demonstrated that PlgOLEs are stress-inducible genes, suggesting their diverse physiological functions in stress responses. These findings shed light on the functional diversity of OLEs in balloon flowers and establish a basis for further research, including genetic modification studies, to elucidate their physiological roles.
참고문헌 (24)
- Bouchnak I, "Lipid droplets are versatile organelles involved in plant development and plant response to environmental changes", Front Plant Sci, 14, pp.1193905~ (2023)
- Buchanan CD, "Sorghum bicolor’s transcriptome response to dehydration, high salinity and ABA", Plant Mol Biol, 58, pp.699~720 (2005)
- Cao H, "Identification, classification and differential expression of oleosin genes in tung tree(Vernicia fordii)", PLoS One, 9, pp.e88409~ (2014)
- Chen K, "Genome-wide identification and functional analysis of oleosin genes in Brassica napus L", BMC Plant Biol, 19, pp.294~ (2019)
- Hyun TK, "Computational identification and phylogenetic analysis of the oil-body structural proteins, oleosin and caleosin, in castor bean and flax", Gene, 515, pp.454~460 (2013)
- Kim HU, "A novel group of oleosins is present inside the pollen of Arabidopsis", J Biol Chem, 277, pp.22677~22684 (2002)
- Kim J, "Wholegenome, transcriptome, and methylome analyses provide insights into the evolution of platycoside biosynthesis in Platycodon grandiflorus, a medicinal plant", Hortic Res, 7, pp.1~12 (2020)
- Kim JS, "An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating droughtinducible genes in Arabidopsis", Plant Cell Physiol, 52, pp.2136~2146 (2011)
- Kyte J, "A simple method for displaying the hydropathic character of a protein", J Mol Biol, 157, pp.105~132 (1982)
- Liu Q, "Species-specific size expansion and molecular evolution of the oleosins in angiosperms", Gene, 509, pp.247~257 (2012)
- Lu J, "The role of triacylglycerol in plant stress response", Plants, 9, pp.472~ (2020)
- Lu Y, "Genome-wide identification, expression profiling, and functional validation of oleosin gene family in Carthamus tinctorius L", Front Plant Sci, 9, pp.1393~ (2018)
- Milioni D, "Gene expression during heat-shock in embryogenic carrot cell lines", Plant Cell Tissue Org Cult, 65, pp.221~228 (2001)
- Parthibane V, "Serine", threonine, Plant Physiol, pp.2012, 159[DOI]
- Parthibane V, "Oleosin is bifunctional enzyme that has both monoacylglycerol acyltransferase and phospholipase activities", J Biol Chem, 287, pp.1946~1954 (2012)
- Shimada TL, "Oil-body-membrane proteins and their physiological functions in plants", Biol Pharm Bull, 33, pp.360~363 (2010)
- Siloto RM, "The accumulation of oleosins determines the size of seed oilbodies in Arabidopsis", Plant Cell, 18, pp.1961~1974 (2006)
- Tai SS, "Gene family of oleosin isoforms and their structural stabilization in sesame seed oil bodies", Biosci Biotechnol Biochem, 66, pp.2146~2153 (2002)
- Wu LSH, "Classification of the single oleosin isoform and characterization of seed oil bodies in gymnosperms", Plant Cell Physiol, 40, pp.326~334 (1999)
- Yin J, "Cloning and expression of BpMYC4 and BpbHLH9 genes and the role of BpbHLH9 in triterpenoid synthesis in birch", BMC Plant Biol, 17, pp.214~ (2017)
- Yuan Y, "Genome-wide identification and analysis of oleosin gene family in four cotton species and its involvement in oil accumulation and germination", BMC Plant Biol, 21, pp.569~ (2021)
- Zhang D, "Artificial selection on GmOLEO1 contributes to the increase in seed oil during soybean domestication", PLoS Genet, 15, pp.e1008267~ (2019)
- Zhi Y, "Comparative lipidomics and proteomics of lipid droplets in the mesocarp and seed tissues of Chinese tallow(Triadica sebifera)", Front Plant Sci, 8, pp.1339~ (2017)
- Zhu Y, "Analysis of oil synthesis pathway in Cyperus esculentus tube rs and identification of oleosin and caleosin genes", J Plant Physiol, 284, pp.153961~ (2023)