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Molecular cloning and characterization of a soybean GmMBY184 induced by abiotic stresses

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초록

Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184(Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

Abstract

Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184(Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.

참고문헌 (37)

  1. Cheong YH, "Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis", Plant Physiol, 129, pp.661~677 (2002)
  2. Doil Choi, "A Method of High Frequency Virus-induced Gene Silencing in Chili Pepper (Capsicum annuum L. cv. Bukang)", Molecules and Cells, 17(2), pp.377~380 (2004), 한국분자세포생물학회
  3. 정은숙, "Molecular cloning and characterization of the soybean DEAD-box RNA helicase gene induced by low temperature and high salinity stress", GENE, 443(1-2), pp.91~99, ELSEVIER SCIENCE BV
  4. Cominelli E, "Overexpression of the Arabidopsis AtMYB41 gene alters cell expansion and leaf surface permeability", Plant J, 53, pp.53~64 (2008)
  5. Ding ZH, "Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana", J Genet Genomics, 36, pp.17~29 (2009)
  6. Dubos C, "MYB transcription factors in Arabidopsis", Trends Plant Sci, 15, pp.573~581 (2010)
  7. Fowler S, "Arabidopsis transcriptome profiling indicates that multiple regulatory pathways are activated during cold acclimation in addition to the CBF cold response pathway", Plant Cell, 14, pp.1675~1690 (2002)
  8. Gocal GFW, "GAMYB-like genes, flowering, and gibberellin signaling in Arabidopsis", Plant Physiol, 127, pp.1682~1693 (2001)
  9. He Y, "Ectopic expression of a wheat MYB transcription factor gene, TaMYB73, improves salinity stress tolerance in Arabidopsis thaliana", J Exp Bot, 63, pp.1511~1522 (2012)
  10. Higgins DG, "Using CLUSTAL for multiple sequence alignments", Methods Enzymol, 266, pp.383~402 (1996)
  11. Himmelbach A, "Relay and control of abscisic acid signaling", Curr Opin Plant Biol, 6, pp.470~479 (2003)
  12. Jin H, "Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol", Plant Mol Biol, 41, pp.577~585 (1999)
  13. Klempnauer KH, "Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: the architecture of a transduced oncogene", Cell, 31, pp.453~463 (1982)
  14. Kranz HD, "Towards functional characterization of the numbers of the R2R3-MYB gene family from Arabidopsis thaliana", Plant J, 16, pp.263~276 (1998)
  15. Liang YK, "AtMYB61, an R2R3-MYB transcription factor controlling stomatal aperture in Arabidopsis thaliana", Curr Biol, 15, pp.1201~1206 (2005)
  16. Liao Y, "Soybean GmMYB76, GmMYB92, and GmMYB177genes confer stress tolerance in transgenic Arabidopsis plants", Cell Res, 18, pp.1047~1060 (2008)
  17. Lipsick JS, "One billion years of Myb", Oncogene, 13, pp.223~235 (1996)
  18. Liu L, "The roles of MYB transcription factors on plant defense responses and its molecular mechanism", Hereditas, 30, pp.1265~1271 (2008)
  19. Martin C, "MYB transcription factors in plants", Trends Genet, 13, pp.67~73 (1997)
  20. Nagaoka S, "Salt tolerance-related protein STO binds to a Myb transcription factor homologue and confers salt tolerance in Arabidopsis", J Exp Bot, 54, pp.2231~2237 (2003)
  21. Nakashima K, "Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses", Plant Physiol, 149, pp.88~95 (2009)
  22. Payne T, "Heterologous myb genes distinct from GL1 enhance trichome production when overexpressed in Nicotiana tabacum", Development, 126, pp.671~682 (1999)
  23. Qin Y, "Overexpression of TaMYB33 encoding a novel wheat MYB transcription factor increases salt and drought tolerance in Arabidopsis", Mol Biol Rep, 39, pp.7183~7192 (2012)
  24. Schmitz G, "The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems", Proc Natl Acad Sci USA, 99, pp.1064~1069 (2002)
  25. Schwechheimer C, "Plant transcription factor studies", Ann Rev Plant Physiol Plant Mol Biol, 49, pp.127~150 (1998)
  26. Shan H, "Heterologous expression of the Chrysanthemum R2R3-MYB transcription factor CmMYB2 enhances drought and salinity tolerance, increases hypersensitivity to ABA and delays flowering in Arabidopsis thaliana", Mol Biotechnol, 51, pp.160~173 (2012)
  27. Shen H-B, "Nuc-PLoc: A new web-server for predicting protein subnuclear localization by fusing PseAA composition and PsePSSM", Protein Eng Des Sel, 20, pp.561~567 (2007)
  28. Shinozaki K, "Gene networks involved in drought stress response and tolerance", J Exp Bot, 58, pp.221~227 (2007)
  29. Singh K, "Transcription factors in plant defense and stress responses", Curr Opin Plant Biol, 5, pp.430~436 (2002)
  30. Stracke R, "The R2R3-MYB gene family in Arabidopsis thaliana", Curr Opin Plant Biol, 4, pp.447~456 (2001)
  31. Tamagnone L, "The AmMYB308 and AmMYB330 transcription factors from antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco", Plant Cell, 10, pp.135~154 (1998)
  32. Urao T, "An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence", Plant Cell, 5, pp.1529~1539 (1993)
  33. Vannini C, "Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants", Plant J, 37, pp.115~127 (2004)
  34. Villalobos MA, "Stress tolerance and glucose insensitive phenotyes in Arabidopsis overexpressing the CpMYB10 transcription factor gene", Plant Physiol, 135, pp.309~324 (2004)
  35. Weston K, "Myb proteins in life, death and differentiation", Curr Opin Genet Dev, 8, pp.76~81 (1998)
  36. Zhang L, "Molecular characterization of 60 isolated wheat MYB genes and analysis of their expression during abiotic stress", J Exp Bot, 63, pp.203~214 (2012)
  37. Zhu JH, "HOS10 encodes an R2R3-type MYB transcription factor essential for cold acclimation in plants", Proc Natl Acad Sci USA, 102, pp.9966~9971 (2005)

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