Bradykinin-induced Ca2+ signaling in human oral squamous cell carcinoma HSC-3 cells
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Cytosolic Ca 2+ is an important regulator of tumor cell proliferation and metastasis. Recently, the strategy of blocking receptors and channels specific to certain cancer cell types has emerged as a potentially viable future treatment. Oral squamous cell carcinoma is an aggressive form of cancer with a high metastasis rate but the receptormechanisms involved in Ca 2+ signaling in these tumors have not yet been elucidated. In our present study, we report that bradykinin induces Ca 2+ signaling and its modulation in the human oral squamous carcinoma cell line, HSC-3. Bradykinin was found to increase the cytosolic Ca 2+ levels in a concentration-dependent manner. This increase was inhibited by pretreatment with the phospholipase C-β inhibitor, U73122, and also by 2-aminoethoxydiphenyl borate, an inhibitor of the inositol 1,4,5-trisphosphate receptor. Pretreatment with extracellular ATP also inhibited the peak bradykinin-induced Ca 2+ rise. In contrast, the ATP-induced rise in cytosolic Ca 2+ was not affected by pretreatment with bradykinin. Pretreatment of the cells with either forskolin or phorbol 12-myristate 13-acetate (activators of adenylyl cyclase and protein kinase C, respectively) prior to bradykinin application accelerated the recovery of cytosolic Ca 2+ to baseline levels. These data suggest that bradykinin receptors are functional in Ca 2+ signaling in HSC-3 cells and may therefore represent a future target in treatment strategies for human oral squamous cell carcinoma.
Abstract
Cytosolic Ca 2+ is an important regulator of tumor cell proliferation and metastasis. Recently, the strategy of blocking receptors and channels specific to certain cancer cell types has emerged as a potentially viable future treatment. Oral squamous cell carcinoma is an aggressive form of cancer with a high metastasis rate but the receptormechanisms involved in Ca 2+ signaling in these tumors have not yet been elucidated. In our present study, we report that bradykinin induces Ca 2+ signaling and its modulation in the human oral squamous carcinoma cell line, HSC-3. Bradykinin was found to increase the cytosolic Ca 2+ levels in a concentration-dependent manner. This increase was inhibited by pretreatment with the phospholipase C-β inhibitor, U73122, and also by 2-aminoethoxydiphenyl borate, an inhibitor of the inositol 1,4,5-trisphosphate receptor. Pretreatment with extracellular ATP also inhibited the peak bradykinin-induced Ca 2+ rise. In contrast, the ATP-induced rise in cytosolic Ca 2+ was not affected by pretreatment with bradykinin. Pretreatment of the cells with either forskolin or phorbol 12-myristate 13-acetate (activators of adenylyl cyclase and protein kinase C, respectively) prior to bradykinin application accelerated the recovery of cytosolic Ca 2+ to baseline levels. These data suggest that bradykinin receptors are functional in Ca 2+ signaling in HSC-3 cells and may therefore represent a future target in treatment strategies for human oral squamous cell carcinoma.
참고문헌 (43)
- Barki-Harrington L, "Requirement for direct cross-talk between B1 and B2 kinin receptors for the proliferation of androgen-insensitive prostate cancer PC3 cells", Biochem J, 371, pp.581~587 (2003)
- Bhoola KD, "Bioregulation of kinins: kallikreins, kininogens, and kininases", Pharmacol Rev, 44, pp.1~80 (1992)
- Chan DC, "Bradykinin antagonist dimer, CU201, inhibits the growth of human lung cancer cell lines in vitro and in vivo and produces synergistic growth inhibition in combination with other antitumor agents", Clin Cancer Res, 8, pp.1280~1287 (2002)
- Choi SY, "Potentiation of PGE2-mediated cAMP production during neuronal differentiation of human neuroblastoma SK-N-BE(2)C cells", J Neurochem, 79, pp.303~310 (2001)
- Chuang HH, "Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5) P2-mediated inhibition", Nature, 411, pp.957~962 (2001)
- Endo Y, "Sarcoendoplasmic reticulum Ca 2+ ATPase type 2 downregulated in human oral squamous cell carcinoma", Int J Cancer, 110, pp.225~231 (2004)
- Ferguson PJ, "Enhancement of platinumdrug cytotoxicity in a human head and neck squamous cell carcinoma line and its platinum-resistant variant by liposomal amphotericin B and phospholipase A2-II", Drug Metab Dispos, 27, pp.1399~1405 (1999)
- Grynkiewicz G, "A new generation of Ca 2+ indicators with greatly improved fluorescence properties", J Biol Chem, 260, pp.3440~3450 (1985)
- Hosoi K, "Bradykinin-stimulated transient modulation of epidermal growth factor receptors in A-431 human epidermoid carcinoma cells", J Cell Physiol, 157, pp.1~12 (1993)
- Hsieh HL, "BK-induced cytosolic phospholipase A2 expression via sequential PKC-δ, p42", p44 MAPK, and NF-κB activation in rat brain astrocytes, 2006, pp.206, 246~254
- Kato H, "Downregulation of 1D-myo-inositol 1,4,5-trisphosphate 3-kinase A protein expression in oral squamous cell carcinoma", Int J Oncol, 28, pp.873~881 (2006)
- Kato Y, "Gene expression pattern in oral cancer cervical lymph node metastasis", Oncol Rep, 16, pp.1009~1014 (2006)
- Kokoska ER, "Epidermal growth factor-induced cytoprotection in human intestinal cells involves intracellular calcium signaling", J Surg Res, 88, pp.97~103 (2000)
- Kurahara S, "Expression of MMPS, MT-MMP, and TIMPs in squamous cell carcinoma of the oral cavity: correlations with tumor invasion and metastasis", Head Neck, 21, pp.627~638 (1999)
- Lai J, "Dynorphin A activates bradykinin receptors to maintain neuropathic pain", Nat Neurosci, 9, pp.1534~1540 (2006)
- Lee WJ, "Lehrbach GM, Monteith GR. Expression of plasma membrane calcium pump isoform mRNAs in breast cancer cell lines", Cell Signal, 14, pp.1015~1022 (2002)
- Liu LH, "Squamous cell tumors in mice heterozygous for a null allele of Atp2a2, encoding the sarco(endo)plasmic reticulum Ca 2+-ATPase isoform 2 Ca 2+ pump", J Biol Chem, 276, pp.26737~26740 (2001)
- Lumenta DB, "Schmid-Elsaesser R, Zausinger S. Neuroprotective effects of a postischemic treatment with a bradykinin B2 receptor antagonist in a rat model of temporary focal cerebral ischemia", Brain Res, 1069, pp.227~234 (2006)
- Mahabeer R, "Kallikrein and kinin receptor genes", Pharmacol Ther, 88, pp.77~89 (2000)
- 민지학, "Mutantional analysis of tumor suppressor gene p53 in human oral squamous carcinoma cell line YD-9", International Journal of Oral Biology, 32(2), pp.79~84 (2007), 대한구강생물학회
- Momose F, "Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas", J Oral Pathol Med, 18, pp.391~395 (1989)
- Monteith GR, "Calcium and cancer: targeting Ca 2+ transport", Nat Rev Cancer, 7, pp.519~530 (2007)
- Myoung H, "Anti-cancer effect of genistein in oral squamous cell carcinoma with respect to angiogenesis and in vitro invasion", Cancer Sci, 94, pp.215~220 (2003)
- Ongali B, "Autoradiographic analysis of rat brain kinin B1 and B2 receptors: normal distribution and alterations induced by epilepsy", J Comp Neurol, 461, pp.506~519 (2003)
- Parkin DM, "Global cancer statistics", CA Cancer J Clin, 49, pp.33~64 (1999)
- Prasad V, "Haploinsufficiency of Atp2a2, encoding thesarco(endo)plasmic reticulum Ca 2+-ATPase isoform 2 Ca 2+ pump, predisposes mice to squamous cell tumors via a novel mode of cancer susceptibility", Cancer Res, 65, pp.8655~8661 (2005)
- Raidoo DM, "Kinin receptors are expressed in human astrocytic tumour cells", Immunopharmacology, 43, pp.255~263 (1999)
- Roderick HL, "Ca 2+ signalling checkpoints in cancer: remodelling Ca 2+ for cancer cell proliferation and survival", Nat Rev Cancer, 8, pp.361~375 (2008)
- Roy SS, "Bad targets the permeability transition pore independent of Bax or Bak to switch between Ca 2+-dependent cell survival and death", Mol Cell, 33, pp.377~388 (2009)
- Saito K, "Plasma membrane Ca 2+ ATPase isoform 1 down-regulated in human oral cancer", Oncol Rep, 15, pp.49~55 (2006)
- Shen X, "Adhesion-mediated squamous cell carcinoma survival through ligand-independent activation of epidermal growth factor receptor", Am J Pathol, 165, pp.1315~1329 (2004)
- 손현진, "Methylation Status and Expression of E-cadherin in Oral Squamous Cell Carcinomas Compared to Benign Oral Epithelial Lesions", International Journal of Oral Biology, 31(2), pp.27~32 (2006), 대한구강생물학회
- Stewart JM, "Bradykinin-related compounds as new drugs for cancer and inflammation", Can J Physiol Pharmacol, 80, pp.275~280 (2002)
- Stewart JM, "Taraseviciene-Stewart L. Combination cancer chemotherapy with one compound: pluripotent bradykinin antagonists", Peptides, 26, pp.1288~1291 (2005)
- Taub JS, "Leeb-Lundberg LM, Madden JF, Daaka Y. Bradykinin receptor subtype 1 expression and function in prostate cancer", Cancer Res, 63, pp.2037~2041 (2003)
- Taylor JT, "Selective blockade of T-type Ca 2+ channels suppresses human breast cancer cell proliferation", Cancer Lett, 267, pp.116~124 (2008)
- Thomas SM, "Epidermal growth factor receptor-stimulated activation of phospholipase Cgamma-1 promotes invasion of head and neck squamous cell carcinoma", Cancer Res, 63, pp.5629~5635 (2003)
- Tsujimoto Y, "Mitochondrial membrane permeability transition and cell death", Biochim Biophys Acta, 1757, pp.1297~1300 (2006)
- Usachev YM, "Differentiation induces up-regulation of plasma membrane Ca 2+-ATPase and concomitant increase in Ca 2+ efflux in human neuroblastoma cell line IMR-32", J Neurochem, 76, pp.1756~1765 (2001)
- Wang SJ, "Hyaluronan and the interaction between CD44 and epidermal growth factor receptor in oncogenic signaling and chemotherapy resistance in head and neck cancer", Arch Otolaryngol Head Neck Surg, 132, pp.771~778 (2006)
- Wang H, "Bradykinin produces pain hypersensitivity by potentiating spinal cord glutamatergic synaptic transmission", J Neurosci, 25, pp.7986~7992 (2005)
- Werry TD, "Mechanisms of crosstalk between G-protein-coupled receptors resulting in enhanced release of intracellular Ca 2+", Biochem J, 374, pp.281~296 (2003)
- Wu J, "Modulation of enhanced vascular permeability in tumors by a bradykinin antagonist, a cyclooxygenase inhibitor, and a nitric oxide scavenger", Cancer Res, 58, pp.159~165 (1998)
