ATP에 의한 자궁경부암 세포내 NF-κB와칼슘이온의 변화
Adenosine 5’-triphosphate induced NF-κB and intracellular calcium mobilization in cervical cancer cells
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초록
본 연구는 ATP에 의한 자궁경부암 세포내 칼슘이온의 변화와 세포증식 효과를 조사하기 위하여 수행되었다. 4종류의 자궁경부암 세포주 (Caski, C33A, HeLaS3 and SiHa) 가 본 연구에서 사용되었으며, 세포질외 ATP에 의한 세포내 칼슘이온의 변화, 세포증식 및 증식과 칼슘이온에 연관된 전사요소가 조사되었다. 결론적으로, ATP는 세포내 칼슘농도의 증가와 세포증식 및 NF-κB의 활성을 유도시켰으며, 따라서, 자궁경부암에 있어서 칼슘농도의 증가와 NF-κB는 ATP에 의한 세포증식에 중요한 역할을 하고 있는 것으로 제시되었다.
Abstract
Objective: To know the effect of adenosine 5’-triphosphate (ATP) on intracellular calcium level and cell proliferation in cervical cancer cells. Methods: Study design: Four different human cervical cancer cell lines (Caski, C33A, HeLaS3 and SiHa) were used in this study. The change of intracellular calcium level, cell proliferation and the activity of proliferation- and calcium-related transcription factors by extracellular ATP were examined in these cell lines. Results: Extracellular ATP induced calcium mobilization, cell proliferation and the activation of NF-κB in all cell lines used. Conclusion: These results suggest that calcium mobilization and NF-κB dependent signaling pathway play an important role in the cell proliferation by ATP in cervical cancer.
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