Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets
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Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.
Abstract
Purpose: The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum. Methods: In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet. Results: The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets. Conclusions: This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.
참고문헌 (40)
- Armitage GC, "Development of a classification system for periodontal diseases and conditions", Ann Periodontol, 4, pp.1~6 (1999)
- Apicella A, "Scaffold requirements for periodontal regeneration with enamel matrix derivative proteins", Colloids Surf B Biointerfaces, 156, pp.221~226 (2017)
- Reynolds MA, "Periodontal regeneration-intrabony defects : a consensus report from the AAP Regeneration Workshop", J Periodontol, 86, pp.105~107 (2015)
- Greenwell HCommittee on Research, Science and Therapy, "American Academy of Periodontology. Position paper: guidelines for periodontal therapy", J Periodontol, 72, pp.1624~1628 (2001)
- Hasegawa M, "Human periodontal ligament cell sheets can regenerate periodontal ligament tissue in an athymic rat model", Tissue Eng, 11, pp.469~478 (2005)
- Bright R, "Periodontal ligament-derived cells for periodontal regeneration in animal models : a systematic review", J Periodontal Res, 50, pp.160~172 (2015)
- Gay IC, "Isolation and characterization of multipotent human periodontal ligament stem cells", Orthod Craniofac Res, 10, pp.149~160 (2007)
- Ishikawa I, "Cell sheet engineering and other novel cell-based approaches to periodontal regeneration", Periodontol 2000, 51, pp.220~238 (2009)
- Xu Q, "Combination of platelet-rich plasma within periodontal ligament stem cell sheets enhances cell differentiation and matrix production", J Tissue Eng Regen Med, 11, pp.627~636 (2017)
- Carter SD, "Additive biomanufacturing : an advanced approach for periodontal tissue regeneration", Ann Biomed Eng, 45, pp.12~22 (2017)
- Okano T, "Thermally on-off switching polymers for drug permeation and release", J Control Release, 11, pp.255~265 (1990)
- Li M, "Effect of cryopreservation on proliferation and differentiation of periodontal ligament stem cell sheets", Stem Cell Res Ther, 8, pp.77~ (2017)
- Guo S, "Periodontal-derived mesenchymal cell sheets promote periodontal regeneration in inflammatory microenvironment", Tissue Eng Part A, 23, pp.585~596 (2017)
- Wang Z, "In vitro studies on human periodontal ligament stem cell sheets enhanced by enamel matrix derivative", Colloids Surf B Biointerfaces, 141, pp.102~111 (2016)
- Dalby MJ, "The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder", Nat Mater, 6, pp.997~1003 (2007)
- Kim DH, "Matrix nanotopography as a regulator of cell function", J Cell Biol, 197, pp.351~360 (2012)
- McMurray RJ, "Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency", Nat Mater, 10, pp.637~644 (2011)
- Gao H, "Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration", Int J Nanomedicine, 10, pp.4009~4027 (2015)
- Jiao A, "Thermoresponsive nanofabricated substratum for the engineering of three-dimensional tissues with layer-by-layer architectural control", ACS Nano, 8, pp.4430~4439 (2014)
- Kim K, "Maintained stemness of human periodontal ligament stem cells isolated after prolonged storage of extracted teeth", J Periodontol, 87, pp.148~158 (2016)
- Farag A, "Decellularized periodontal ligament cell sheets with recellularization potential", J Dent Res, 93, pp.1313~1319 (2014)
- Hu J, "Periodontal regeneration in swine after cell injection and cell sheet transplantation of human dental pulp stem cells following good manufacturing practice", Stem Cell Res Ther, 7, pp.130~ (2016)
- Dan H, "The influence of cellular source on periodontal regeneration using calcium phosphate coated polycaprolactone scaffold supported cell sheets", Biomaterials, 35, pp.113~122 (2014)
- Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
- Lemaitre M, "Periodontal tissue regeneration using syngeneic adipose-derived stromal cells in a mouse model", Stem Cells Transl Med, 6, pp.656~665 (2017)
- Wang ZS, "The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering", Sci Rep, 6, pp.28126~ (2016)
- Feng R, "Application of stem cell technology in dental regenerative medicine", Adv Wound Care(New Rochelle), 2, pp.296~305 (2013)
- Du J, "Allogeneic bone marrow mesenchymal stem cell transplantation for periodontal regeneration", J Dent Res, 93, pp.183~188 (2014)
- Owaki T, "Cell sheet engineering for regenerative medicine : current challenges and strategies", Biotechnol J, 9, pp.904~914 (2014)
- Kim JH, "Dynamic mechanical and nanofibrous topological combinatory cues designed for periodontal ligament engineering", PLoS One, 11, pp.e0149967~ (2016)
- Yin Z, "The regulation of tendon stem cell differentiation by the alignment of nanofibers", Biomaterials, 31, pp.2163~2175 (2010)
- Jiang W, "Incorporation of aligned PCL-PEG nanofibers into porous chitosan scaffolds improved the orientation of collagen fibers in regenerated periodontium", Acta Biomater, 25, pp.240~252 (2015)
- Gauvin R, "Dynamic mechanical stimulations induce anisotropy and improve the tensile properties of engineered tissues produced without exogenous scaffolding", Acta Biomater, 7, pp.3294~3301 (2011)
- Yu N, "Regulation of periodontal ligament cell behavior by cyclic mechanical loading and substrate nanotexture", J Periodontol, 84, pp.1504~1513 (2013)
- San Miguel SM, "Defining a visual marker of osteoprogenitor cells within the periodontium", J Periodontal Res, 45, pp.60~70 (2010)
- Kalajzic Z, "Use of an alpha-smooth muscle actin GFP reporter to identify an osteoprogenitor population", Bone, 43, pp.501~510 (2008)
- Jin H, "Recombinant human plasminogen activator inhibitor-1 promotes cementogenic differentiation of human periodontal ligament stem cells", Tissue Eng Part A, 21, pp.2817~2828 (2015)
- Zhu B, "Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells", Sci Rep, 7, pp.40088~ (2017)
- Kadkhoda Z, "Histopathological comparison between bone marrow-and periodontium-derived stem cells for bone regeneration in rabbit calvaria", Int J Organ Transplant Med, 7, pp.9~18 (2016)
- Scott A, "Mechanical force modulates scleraxis expression in bioartificial tendons", J Musculoskelet Neuronal Interact, 11, pp.124~132 (2011)
