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Characterization of Odontoblasts in Supernumerary Tooth-derived Dental Pulp Stem Cells between Passages by Real-Time PCR

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The aim of this study is to compare the properties of odontoblast gene of early passage cells and late passage cells derived from impacted maxillary supernumerary teeth. Impacted supernumerary teeth with maxilla were extracted from 12 patients (8 males, 4 females) between 6 - 9 years old without medical history. Real-time polymerase chain reaction (PCR) was conducted to compare characterization of odontoblast cell in the 3rd and 10th passage, and between with bone inducing additive group and without additive group. Genes for odontoblasts characteristics are osteonectin (ONT), alkaline phosphatase (ALP), osteocalcin (OCN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). The level of gene expression was in a decreasing order of ONT, ALP, OCN, DMP-1 and DSPP in the 3rd passage, and in decreasing order of ONT, DMP-1, OCN, ALP, and DSPP in the 10th passage in the undifferentiation and differentiation group. The order of ONT, DMP-1, and OCN did not changed. ALP and DMP-1 were switched in order. ALP and DMP-1 may be used as important markers for differentiating between the 3rd passage and 10th passage cells. Considering that supernumerary tooth was extracted young age and the time required to cultured 10th passage was short, supernumerary tooth can be considered a useful donor site of dental pulp stem cells.

Abstract

The aim of this study is to compare the properties of odontoblast gene of early passage cells and late passage cells derived from impacted maxillary supernumerary teeth. Impacted supernumerary teeth with maxilla were extracted from 12 patients (8 males, 4 females) between 6 - 9 years old without medical history. Real-time polymerase chain reaction (PCR) was conducted to compare characterization of odontoblast cell in the 3rd and 10th passage, and between with bone inducing additive group and without additive group. Genes for odontoblasts characteristics are osteonectin (ONT), alkaline phosphatase (ALP), osteocalcin (OCN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). The level of gene expression was in a decreasing order of ONT, ALP, OCN, DMP-1 and DSPP in the 3rd passage, and in decreasing order of ONT, DMP-1, OCN, ALP, and DSPP in the 10th passage in the undifferentiation and differentiation group. The order of ONT, DMP-1, and OCN did not changed. ALP and DMP-1 were switched in order. ALP and DMP-1 may be used as important markers for differentiating between the 3rd passage and 10th passage cells. Considering that supernumerary tooth was extracted young age and the time required to cultured 10th passage was short, supernumerary tooth can be considered a useful donor site of dental pulp stem cells.

참고문헌 (37)

  1. Linde A, "Dentinogenesis", Crit Rev Oral Biol Med, 4, pp.679~728 (1993)
  2. Sasaki T, "Structure and organization of odontoblasts", Anat Rec, 245, pp.235~249 (1996)
  3. Bègue-Kirn C, "Comparative analysis of TGF beta s, BMPs, IGF1, msxs, fibronectin, osteonectin and bone sialoprotein gene expression during normal and in vitro-induced odontoblast differentiation", Int J Dev Biol, 38, pp.405~420 (1994)
  4. Papagerakis P, "Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth", Bone, 30, pp.377~385 (2002)
  5. Gronthos S, "Postnatal human dental pulp stem cells(DPSCs)in vitro and in vivo", Proc Natl Acad Sci USA, 97, pp.13625~13630 (2000)
  6. Lindroos B, "Characterisation of human dental stem cells and buccal mucosa fibroblasts", Biochem Biophys Res Commun, 368, pp.329~335 (2008)
  7. Miura M, "SHED : stem cells from human exfoliated deciduous teeth", Proc Natl Acad Sci USA, 100, pp.5807~5812 (2003)
  8. Huang AH, "Isolation and characterization of dental pulp stem cells from a supernumerary tooth", J Oral Pathol Med, 37, pp.571~574 (2008)
  9. Sun HJ, "A proteomic analysis during serial subculture and osteogenic differentiation of human mesenchymal stem cell", J Orthop Res, 24, pp.2059~2071 (2006)
  10. 김종수, "과잉치 치수유래 줄기세포의 분화제 처리 기간에 따른 상아모세포 발현 특성", 대한소아치과학회지, 42(4), pp.312~318 (2015), 대한소아치과학회[DOI]
  11. Primosch RE, "Anterior supernumerary teeth-assessment and surgical intervention in children", Pediatr Dent, 3, pp.204~215 (1981)
  12. Huang WH, "Mesiodens in the primary dentition stage : a radiographic study", ASDC J Dent Child, 59, pp.186~189 (1992)
  13. 김종빈, "Managing Complications Related to Multiple Supernumerary Teeth", 대한소아치과학회지, 41(2), pp.180~186 (2014), 대한소아치과학회[DOI]
  14. Gronthos S, "Stem cell properties of human dental pulp stem cells", J Dent Res, 81, pp.531~535 (2002)
  15. Bruder SP, "Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation", J Cell Biochem, 64, pp.278~294 (1997)
  16. Digirolamo CM, "Propagation and senescence of human marrow stromal cells in culture : a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate", Br J Haematol, 107, pp.275~281 (1999)
  17. Min JH, "Dentinogenic potential of human adult dental pulp cells during the extended primary culture", Hum Cell, 24, pp.43~50 (2011)
  18. Livak KJ, "Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T))Method", Methods, 25, pp.402~408 (2001)
  19. Seo BM, "Investigation of multipotent postnatal stem cells from human periodontal ligament", Lancet, 364, pp.149~155 (2004)
  20. Morsczeck C, "Isolation of precursor cells(PCs)from human dental follicle of wisdom teeth", Matrix Biol, 24, pp.155~165 (2005)
  21. Sonoyama W, "Mesenchymal stem cellmediated functional tooth regeneration in swine", PLoS One, 1, pp.79~ (2006)
  22. Huang W, "Mesiodens in the primary dentition stage : a radiographic study", ASDC J Dent Child, 59, pp.186~189 (1991)
  23. Kamata N, "Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase", J Oral Pathol Med, 33, pp.417~423 (2004)
  24. Conget PA, "Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells", J Cell Physiol, 181, pp.67~73 (1999)
  25. Aubin JE, "Osteoblast and chondroblast differentiation", Bone, 17, pp.S77~S83 (1995)
  26. Arana-Chavez VE, "Odontoblasts : the cells forming and maintaining dentine", Int J Biochem Cell Biol, 36, pp.1367~1373 (2004)
  27. Termine JD, "Osteonectin, a bone-specific protein linking mineral to collagen", Cell, 26, pp.99~105 (1981)
  28. Wasi S, "An osteonectinlike protein in porcine periodontal ligament and its synthesis by periodontal ligament fibroblasts", Can J Biochem Cell Biol, 62, pp.470~478 (1984)
  29. Garcia JM, "Immunolocalization of bone extracellular matrix proteins(type I collagen, osteonectin and bone sialoprotein)in human dental pulp and cultured pulp cells", Int Endod J, 36, pp.404~410 (2003)
  30. Lauc G, "Shedding and uptake of gangliosides and glycosylphosphatidylinositol-anchored proteins", Biochim Biophys Acta, 1760, pp.584~602 (2006)
  31. Hanawa M, "An autoradiographic study of calcium movement in the enamel organ of rat molar tooth germs", Arch Oral Biol, 35, pp.899~906 (1990)
  32. Bronckers AL, "Studies of osteocalcin function in dentin formation in rodent teeth", Eur J Oral Sci, 106, pp.795~807 (1998)
  33. Karsenty G, "Role of Cbfa1 in osteoblast differentiation and function", Semin Cell Dev Biol, 11, pp.343~346 (2000)
  34. George A, "Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization", J Biol Chem, 268, pp.12624~12630 (1993)
  35. D'souza RN, "Gene expression patterns of murine dentin matrix protein 1(Dmp1)and dentin sialophosphoprotein(DSPP)suggest distinct developmental functions in vivo", J Bone Miner Res, 12, pp.2040~2049 (1997)
  36. Rutherford RB, "Induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1", Arch Oral Biol, 38, pp.571~576 (1993)
  37. Feng JQ, "Genomic organization, chromosomal mapping, and promoter analysis of the mouse dentin sialophosphoprotein(Dspp)gene, which codes for both dentin sialoprotein and dentin phosphoprotein", J Biol Chem, 273, pp.9457~9464 (1998)

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