Identification and Antibiotic Susceptibility of the Bacteria from Non-odontogenic Infectious Lesions
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The purpose of this study was to isolate and identifybacteria from the 4 patients with non-odontogenic infectiouslesions (mucormycosis, chronic inflammation from woundinfection, and two actinomycosis) and determine theirantimicrobial susceptibility against eight antibiotics. Bacterial culture was performed under three cultureconditions (anaerobic, CO2, and aerobic incubator). Thebacterial strains were identified by 16S rRNA gene (16SrDNA) sequence comparison analysis method. Forinvestigating the antimicrobial susceptibility of the bacteriaagainst eight antibiotics, penicillin G, amoxicillin,tetracycline, cefuroxime, erythromycin, clindamycin, vancomycin, and Augmentin® (amoxicillin + clavulanicacid), minimum inhibitory concentration (MIC)measurement was performed using broth microdilutionassay. Nosocomial pathogens such as Enterococcus faecalis,Klebsiella pneumoniae, Bacillus subtilis, and Neisseriaflavescens were isolated from mucormycosis. Veillonellaparvula, Enterobacter hormaechei, and Acinetobactercalcoaceticus were isolated from chronic inflammatorylesion. Actinomyces massiliensis was isolated fromactinomycosis in parotid gland. Capnocytophaga ochraceawas isolated from actinomycosis in buccal region inanaerobic condition. There was no susceptible antibiotic toall bacteria in mucormycosis. Tetracycline was susceptibleto all bacteria in chronic inflammation. C. ochracea wasresistant to vancomycin and penicillin G; and otherantibiotics showed susceptibility to all bacteria inactinomycosis. The results indicated that the combinedtreatment of two or more antibiotics is better than singleantibiotic treatment in mucormycosis, and penicillin is thefirst recommended antibiotic to treat actinomycosis.
Abstract
The purpose of this study was to isolate and identifybacteria from the 4 patients with non-odontogenic infectiouslesions (mucormycosis, chronic inflammation from woundinfection, and two actinomycosis) and determine theirantimicrobial susceptibility against eight antibiotics. Bacterial culture was performed under three cultureconditions (anaerobic, CO2, and aerobic incubator). Thebacterial strains were identified by 16S rRNA gene (16SrDNA) sequence comparison analysis method. Forinvestigating the antimicrobial susceptibility of the bacteriaagainst eight antibiotics, penicillin G, amoxicillin,tetracycline, cefuroxime, erythromycin, clindamycin, vancomycin, and Augmentin® (amoxicillin + clavulanicacid), minimum inhibitory concentration (MIC)measurement was performed using broth microdilutionassay. Nosocomial pathogens such as Enterococcus faecalis,Klebsiella pneumoniae, Bacillus subtilis, and Neisseriaflavescens were isolated from mucormycosis. Veillonellaparvula, Enterobacter hormaechei, and Acinetobactercalcoaceticus were isolated from chronic inflammatorylesion. Actinomyces massiliensis was isolated fromactinomycosis in parotid gland. Capnocytophaga ochraceawas isolated from actinomycosis in buccal region inanaerobic condition. There was no susceptible antibiotic toall bacteria in mucormycosis. Tetracycline was susceptibleto all bacteria in chronic inflammation. C. ochracea wasresistant to vancomycin and penicillin G; and otherantibiotics showed susceptibility to all bacteria inactinomycosis. The results indicated that the combinedtreatment of two or more antibiotics is better than singleantibiotic treatment in mucormycosis, and penicillin is thefirst recommended antibiotic to treat actinomycosis.
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