Identification of the Bacteria Isolated from Oral Cavities in Korea
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The aim of this study was to identify bacteria isolated fromthe oral cavities and to determine their antimicrobialsusceptibility against eight antibiotics. The bacterial strainswere obtained from the Korean Collection for OralMicrobiology (KCOM). The bacteria were identified bycomparing 16S rDNA sequences at the species level. The datashowed that 77 bacterial strains were predominantly identifiedas streptococci (49.4%) and staphylococci (14.3%). Minimuminhibitory concentrations (MIC) were determined using abroth dilution assay to test the sensitivity of the bacterialstrains. The MIC values of the oral bacterial strains againstantibiotics were different. Streptococci were sensitive toclindamycin, cefuroxime axetil, and vancomycin, and theywere resistant to tetracycline. Staphylococci also weresensitive to clindamycin, cefuroxime axetil, and vancomycin,and they were resistant to penicillin antibiotics. Gramnegativebacterial strains were sensitive to tetracycline andwere resistant to clindamycin. These results suggest that theantimicrobial susceptibility test is necessary in deciding theprescription for antibiotics, to prevent the misuse or abuse ofantibiotics.
Abstract
The aim of this study was to identify bacteria isolated fromthe oral cavities and to determine their antimicrobialsusceptibility against eight antibiotics. The bacterial strainswere obtained from the Korean Collection for OralMicrobiology (KCOM). The bacteria were identified bycomparing 16S rDNA sequences at the species level. The datashowed that 77 bacterial strains were predominantly identifiedas streptococci (49.4%) and staphylococci (14.3%). Minimuminhibitory concentrations (MIC) were determined using abroth dilution assay to test the sensitivity of the bacterialstrains. The MIC values of the oral bacterial strains againstantibiotics were different. Streptococci were sensitive toclindamycin, cefuroxime axetil, and vancomycin, and theywere resistant to tetracycline. Staphylococci also weresensitive to clindamycin, cefuroxime axetil, and vancomycin,and they were resistant to penicillin antibiotics. Gramnegativebacterial strains were sensitive to tetracycline andwere resistant to clindamycin. These results suggest that theantimicrobial susceptibility test is necessary in deciding theprescription for antibiotics, to prevent the misuse or abuse ofantibiotics.
참고문헌 (42)
- J. A. Aas, "Defining the Normal Bacterial Flora of the Oral Cavity", Journal of Clinical Microbiology, 43(11), pp.5721~5732 (2005), American Society for Microbiology[DOI]
- B. J. Paster, "Bacterial Diversity in Human Subgingival Plaque", Journal of Bacteriology, 183(12), pp.3770~3783 (2001), American Society for Microbiology[DOI]
- F. E. Dewhirst, "The Human Oral Microbiome", Journal of Bacteriology, 192(19), pp.5002~5017 (2010), American Society for Microbiology[DOI]
- Anne D. Haffajee, "Microbial etiological agents of destructive periodontal diseases", Periodontology 2000, 5(1), pp.78~111 (1994), Wiley-Blackwell[DOI]
- Tatsuji Nishihara, "Microbial etiology of periodontitis", Periodontology 2000, 36(1), pp.14~26 (2004), Wiley-Blackwell[DOI]
- Jorgen Slots, "Subgingival microflora and periodontal disease", Journal of Clinical Periodontology, 6(5), pp.351~382 (1979), Wiley-Blackwell[DOI]
- Whiley RA, "Current classification of the oral streptococci", Oral Microbiol Immunol, 13, pp.195~216 (1998)
- Berbari EF, "Infective endocarditis due to unusual or fastidious microorganism", Mayo Clin Proe, 72, pp.532~542 (1997)
- Nurcan Buduneli, "Periodontal infections and pre-term low birth weight: a case-control study", Journal of Clinical Periodontology, 32(2), pp.174~181 (2005), Wiley-Blackwell[DOI]
- Gupta S, "Chronic infection in the aetiology of atherosclerosisfocus on Chlamydia pneumoniae", Atherosclerosis, 143, pp.1~6 (1999)
- V.I. Haraszthy, "Identification of Periodontal Pathogens in Atheromatous Plaques", Journal of Periodontology, 71(10), pp.1554~1560 (2000), American Academy of Periodontology (AAP)[DOI]
- Offenbacher S, "Potential pathogenic mechanisms of periodontitis associated pregnancy complications", Ann Periodontol, 3, pp.233~250 (1998)
- Aracil B, "High prevalence or erythromycin-resistant and clindamycin-susceptible (M pheno-type) viridans group streptococci from pharyngeal samples: a reservoir of mef genes in commensal bacteria", J Antimicrob Chemother, 48, pp.592~594 (2001)[DOI]
- Groppo FC, "Antimicrobial resistance of Staphylococcus aureus and oral streptococci strains from high-risk ndocarditis patients", Gen Dent, 53, pp.410~413 (2005)
- A. D. Haffajee, "Subgingival microbiota of chronic periodontitis subjects from different geographic locations", Journal of Clinical Periodontology, 31(11), pp.996~1002 (2004), Wiley-Blackwell[DOI]
- A. D. Haffajee, "Differences in the subgingival microbiota of Swedish and USA subjects who were periodontally healthy or exhibited minimal periodontal disease", Journal of Clinical Periodontology, 32(1), pp.33~39 (2005), Wiley-Blackwell[DOI]
- Wilson K., "Current Protocols in Molecular Biology", pp.241~245 (1990), Greene Publ. Assoc. and Wiley Interscience
- Lane DJ, "Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis", Proc Natl Acad Sci. USA, 82, pp.6955~6959 (1985)
- 김용민, "Identification and Antibiotic Susceptibility of the Bacteria from Non-odontogenic Infectious Lesions", International Journal of Oral Biology, 39(2), pp.87~95 (2014), 대한구강생물학회[DOI]
- Jorgensen JH, "Manual of CLINICAL MICROBIOLOGY", pp.1526~1543 (1999), ASM press
- Clinical and Laboratory Standards Institute, "Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically" (2007), Wayne
- La Scola B, "Molecular identification of Gemella species from three patients with endocarditis", J Clin Microbiol, 36, pp.866~871 (1998)
- May T, "Meningitis due to Gemella haemolysans", Eur J Clin Microbiol Infect Dis, 12, pp.644~645 (1993)
- Yumi Nonaka, "Pyogenic vertebral osteomyelitis caused by Gemella haemolysans.", Nihon Naika Gakkai Zasshi, 89(5), pp.980~982 (2000), Japanese Society of Internal Medicine[DOI]
- van Dijk M, "Trochanter osteomyelitis and ipsilateral arthritis due to Gemella morbillorum", Eur J Clin Microbiol Infect Dis, 18, pp.600~602 (1999)
- Kong R, "Case of triple endocarditis caused by Rothia dentocariosa and results of a survey in France", J Clin Microbiol, 36, pp.309~310 (1998)
- Julie Larkin, "Rothia dentocariosa Endocarditis", Clinical Microbiology Newsletter, 23(2), pp.13~15 (2001), Elsevier BV[DOI]
- Soto-Hernandez JL, "Cranial epidural empyema with esteomyelitis caused by actinomyces, CT, and MRI appearance", Clin Imaging, 23, pp.209~214 (1999)
- Vandevelde AG, "Sclerosing osteomyelitis and Actinomyces naeslundii infection of surrounding tissues", Clin Infect Dis, 20, pp.1037~1039 (1995)
- Friedlander AH, "Proteus vulgaris osteomyelitis of the mandible Report of a case", Oral Surg Oral Med Oral Pathol, 40, pp.39~44 (1975)
- Kawamura Y, "Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus", Int J Syst Bacteriol, 45, pp.406~408 (1995)
- 박순낭, "Molecular Identification of Anginosus Group Streptococci Isolated from Korean Oral Cavities", International Journal of Oral Biology, 38(1), pp.21~27 (2013), 대한구강생물학회[DOI]
- Bǎncescu G, "Antimicrobial susceptibility of some streptococci strains of anginosus group isolated from oral and maxillofacial infections", Roum Arch Microbiol Immunol, 58, pp.57~63 (1999)
- Waxman DJ, "Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics", Annu Rev Biochem, 52, pp.825~869 (1983)
- Mark S Wilke, "β-Lactam antibiotic resistance: a current structural perspective", Current Opinion in Microbiology, 8(5), pp.525~533 (2005), Elsevier BV[DOI]
- Bantar C, "Species belonging to the"Streptococcus milleri"group : antimicrobial susceptibility and comparative prevalence in significant clinical specimens", J Clin Microbiol, 34, pp.2020~2022 (1996)
- 신경민, "Antimicrobial Susceptibility of Viridans Streptococci Plaque Isolates in Korea", International Journal of Oral Biology, 33(4), pp.197~203 (2008), 대한구강생물학회
- X. Wang, "The Structural Bases of Antibiotic Resistance in the Clinically Derived Mutant beta -Lactamases TEM-30, TEM-32, and TEM-34", Journal of Biological Chemistry, 277(35), pp.32149~32156 (2002), American Society for Biochemistry & Molecular Biology (ASBMB)[DOI]
- Azzaq Belaaouaj, "Nucleotide sequences of the genes coding for the TEM-like β-lactamases IRT-1 and IRT-2 (formerly called TRI-1 and TRI-2)", FEMS Microbiology Letters, 120(1-2), pp.75~80 (1994), Oxford University Press (OUP)[DOI]
- I. Chopra, "Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance", Microbiology and Molecular Biology Reviews, 65(2), pp.232~260 (2001), American Society for Microbiology[DOI]
- Marily C. Roberts, "Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution", FEMS Microbiology Reviews, 19(1), pp.1~24 (1996), Oxford University Press (OUP)[DOI]
- A. Villedieu, "Prevalence of Tetracycline Resistance Genes in Oral Bacteria", Antimicrobial Agents and Chemotherapy, 47(3), pp.878~882 (2003), American Society for Microbiology[DOI]
